Isolation and Characterization of Phosphate Solubilizing Streptomyces Endemic From Sugar Beet Fields Of the Beni-Mellal Region In Morocco

In this research, we isolated and characterized Streptomyces strains, endemic from sugar beet fields of the Beni-Mellal region, able to use natural rock phosphate (RP) and tricalcium phosphate (TCP), as sole phosphate source. Ten Streptomyces isolates yielded a comparable biomass in the presence of these two insoluble phosphate sources, indicating that they were able to extract similar amount of phosphorus (P) from the latter for their own growth. Interestingly, five strains released soluble P in large excess from TCP in their culture broth whereas only two strains, BP, related to Streptomyces bellus and BYC, related to Streptomyces enissocaesilis, released a higher or similar amount of soluble P from RP than from TCP, respectively. This indicated that the rate of P released from these insoluble phosphate sources exceeded its consumption rate for bacterial growth and that most strains solubilized TCP more efficiently than RP. Preliminary results suggested that the solubilization process of BYC, the most efficient RP and TCP solubilizing strain, involves both acidification of the medium and excretion of siderophores. Actinomycete strains possessing such interesting RP solubilizing abilities may constitute a novel kind of intrans beneficial for plant nutrition and more environmentally friendly than chemical fertilizers in current use.

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Isolation and Characterization of Phosphate Solubilizing Streptomyces sp. Endemic from Sugar Beet Fields of the Beni-Mellal Region in Morocco

Microorganisms ◽

10.3390/microorganisms9050914 ◽

2021 ◽

Vol 9 (5) ◽

pp. 914

Author(s):

Yassine Aallam ◽

Driss Dhiba ◽

Sanaâ Lemriss ◽

Amal Souiri ◽

Fatma Karray ◽

...

Keyword(s):

Sugar Beet ◽

Culture Broth ◽

Similar Amount ◽

Streptomyces Sp ◽

Isolation And Characterization ◽

Natural Rock ◽

Insoluble Phosphate ◽

Beni Mellal ◽

Soluble P ◽

Phosphate Solubilizing

In the course of our research, aimed at improving sugar beets phosphorus nutrition, we isolated and characterized Streptomyces sp. strains, endemic from sugar beet fields of the Beni-Mellal region, which are able to use natural rock phosphate (RP) and tricalcium phosphate (TCP) as sole phosphate sources. Ten Streptomyces sp. isolates yielded a comparable biomass in the presence of these two insoluble phosphate sources, indicating that they were able to extract similar amount of phosphorus (P) from the latter for their own growth. Interestingly, five strains released soluble P in large excess from TCP in their culture broth whereas only two strains, BP, related to Streptomyces bellus and BYC, related to Streptomyces enissocaesilis, released a higher or similar amount of soluble P from RP than from TCP, respectively. This indicated that the rate of P released from these insoluble phosphate sources exceeded its consumption rate for bacterial growth and that most strains solubilized TCP more efficiently than RP. Preliminary results suggested that the solubilization process of BYC, the most efficient RP and TCP solubilizing strain, involves both acidification of the medium and excretion of siderophores. Actinomycete strains possessing such interesting RP solubilizing abilities may constitute a novel kind of fertilizers beneficial for plant nutrition and more environmentally friendly than chemical fertilizers in current use.

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Isolation, identification and characterization of phosphate solubilizing bacteria in soils of coal mines landfills of Chhattisgarh

ANNALS OF PLANT AND SOIL RESEARCH ◽

10.47815/apsr.2020.10018 ◽

2020 ◽

pp. 437-441

Author(s):

NELSON XESS

Keyword(s):

Pseudomonas Syringae ◽

Tricalcium Phosphate ◽

Rock Phosphate ◽

Coal Mines ◽

Soil Health ◽

Phosphate Solubilizing Bacteria ◽

Phosphate Solubilizers ◽

Soluble P ◽

Phosphate Solubilizing ◽

Phosphate Source

Phosphate solubilizing bacteria have the ability to solubilize the insoluble phosphates and to improve the quality of soil health and fertility. Efficacy of phosphate solubilizing microorganisms has been identified on the basis of kinetics and phosphorus accumulation. In this study, twenty eight soil samples were collected from different coal mines landfills of Chhattisgarh and occurrence of phosphate solubilizing bacteria (PSB) was isolated, purified and identified. In addition, the phosphate solubilizing capacity of bacteria based on the formation of visible or halo zone on Pikovskaya agar plates (PVK) and broth having tricalcium phosphate (TCP) and rock phosphate (RP) as a phosphate source was estimated at Dr. C.V. Raman University, Kota, Bilaspur (Chhattisgarh). The result revealed dominance of Pseudomonas syringae as major phosphate solubilizers, along with Bacillus subtillis followed by Pantoea dispersa, Bacillus circulans. Use of these PSB as bioinoculants increased the available P in soil to the extent of 194 and 246 µg/ml of P. the soluble P in case of rock phosphate was less than tricalcium phosphate. The maximum solubilization was observed after 15 days follow by a decrease in amount of soluble P.

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Isolation and characterization of phosphate solubilizing bacteria from the rhizospheric soil of Ponthenpuzha forest, Pathanamthitta (District), Kerala

Research Journal of Biotechnology ◽

10.25303/168rjbt11021 ◽

2021 ◽

Vol 16 (8) ◽

pp. 110-117

Author(s):

Kannan Abhirami ◽

K. Jayakumar

Keyword(s):

Bacterial Isolate ◽

Phosphate Solubilizing Bacteria ◽

Cymbopogon Citratus ◽

16S Rrna Sequence ◽

Isolation And Characterization ◽

16S Rrna Sequence Analysis ◽

Phosphate Solubilizing ◽

Level Identification

Phosphorous is considered as a major parameter for crop yield. Its availability to plant is independent of its abundance. For the plants to utilize phosphorous, it is to be converted to absorbable form. Here, the part rendered by phosphate solubilizing bacteria is significant for it plays a crucial role in the formation of plant usable phosphate from organic forms. In the present work, an effort had been made to isolate and identify phosphate solubilising bacterial isolate from the rhizhospheric soils of various plants in Ponthenpuzha forest. One of the isolate from Cymbopogon citrates responded positively to Pikovskaya’s medium by producing a halo zone during in vitro culture. Colony features and 16S rRNA sequence analysis identified the isolate as Burkholderia sps. We have reported the presence of genus Burkholderia in the rhizospheric zone of Cymbopogon citratus. Further studies are warranted for species level identification of the isolate.

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Isolation and characterization of phosphate solubilizing bacteria from agricultural soils for a potential use in cultivating Capsicum frutescens

Biointerface Research in Applied Chemistry ◽

10.33263/briac102.161173 ◽

2020 ◽

Vol 10 (2) ◽

pp. 5161-5173

Keyword(s):

Plant Growth ◽

Agricultural Soils ◽

Pot Experiment ◽

Available Phosphorus ◽

Phosphate Solubilizing Bacteria ◽

Capsicum Frutescens ◽

Isolation And Characterization ◽

Insoluble Form ◽

Phosphate Solubilizing

Phosphorus (P) is one of the essential macronutrients needed for the plant growth, other than nitrogen and potassium. Most phosphorus remains as insoluble form in soils and the plants only can uptake the phosphorus nutrient in soluble forms. Phosphate solubilizing bacteria (PSB) dissolves the phosphorus and make it available for plants. In this study, Soil samples were collected and screened for PSB on PK medium. PSB colonies with the highest phosphate solubilization ability were chosen and used for studying its rhizosphere effect on Capsicum frutescens by pot experiment.. It was evidenced that selected PSB strain could solubilize phosphate in PK medium and modified PK broth. Besides, it provided available phosphorus for plants and enhanced the plant growth in pot experiment.

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Phosphate-Solubilizing Potentiality of the Microorganisms Capable of Utilizing Aluminium Phosphate as a Sole Phosphate Source

Zentralblatt für Mikrobiologie ◽

10.1016/s0232-4393(83)80060-2 ◽

1983 ◽

Vol 138 (1) ◽

pp. 17-23 ◽

Cited By ~ 38

Author(s):

S. Banik ◽

B.K. Dey

Keyword(s):

Aluminium Phosphate ◽

Phosphate Solubilizing ◽

Phosphate Source

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Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

Polish Journal of Microbiology ◽

10.5604/17331331.1234993 ◽

2017 ◽

Vol 66 (1) ◽

pp. 57-65 ◽

Cited By ~ 19

Author(s):

Jian Zhang ◽

Peng Cheng Wang ◽

Ling Fang ◽

Qi-An Zhang ◽

Cong Sheng Yan ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Wood Flour ◽

Dry Weight ◽

Rrna Gene ◽

Plant Growth Promoting Bacteria ◽

Phosphate Solubilizing Bacteria ◽

Sequence Comparisons ◽

Isolation And Characterization ◽

Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

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