Isolation and characterization of phosphate-solubilizing bacteria from betel nut (Areca catechu) and their effects on plant growth and phosphorus mobilization in tropical soils

2014 ◽  
Vol 50 (6) ◽  
pp. 927-937 ◽  
Author(s):  
Fu-Ping Liu ◽  
Hai-Qing Liu ◽  
Hai-Long Zhou ◽  
Zhi-Guo Dong ◽  
Xu-Hao Bai ◽  
...  
Keyword(s):  
Plant Growth ◽  
Tropical Soils ◽  
Phosphate Solubilizing Bacteria ◽  
Betel Nut ◽  
Isolation And Characterization ◽  
Areca Catechu ◽  
Phosphate Solubilizing

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from agricultural soils for a potential use in cultivating Capsicum frutescens

2020 ◽  
Vol 10 (2) ◽  
pp. 5161-5173
Keyword(s):  
Plant Growth ◽  
Agricultural Soils ◽  
Pot Experiment ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Capsicum Frutescens ◽  
Isolation And Characterization ◽  
Insoluble Form ◽  
Phosphate Solubilizing

Phosphorus (P) is one of the essential macronutrients needed for the plant growth, other than nitrogen and potassium. Most phosphorus remains as insoluble form in soils and the plants only can uptake the phosphorus nutrient in soluble forms. Phosphate solubilizing bacteria (PSB) dissolves the phosphorus and make it available for plants. In this study, Soil samples were collected and screened for PSB on PK medium. PSB colonies with the highest phosphate solubilization ability were chosen and used for studying its rhizosphere effect on Capsicum frutescens by pot experiment.. It was evidenced that selected PSB strain could solubilize phosphate in PK medium and modified PK broth. Besides, it provided available phosphorus for plants and enhanced the plant growth in pot experiment.

Isolation and characterization of phosphate solubilizing bacteria from the rhizospheric soil of Ponthenpuzha forest, Pathanamthitta (District), Kerala

2021 ◽  
Vol 16 (8) ◽  
pp. 110-117
Author(s):  
Kannan Abhirami ◽  
K. Jayakumar
Keyword(s):  
Bacterial Isolate ◽  
Phosphate Solubilizing Bacteria ◽  
Cymbopogon Citratus ◽  
16S Rrna Sequence ◽  
Isolation And Characterization ◽  
16S Rrna Sequence Analysis ◽  
Phosphate Solubilizing ◽  
Level Identification

Phosphorous is considered as a major parameter for crop yield. Its availability to plant is independent of its abundance. For the plants to utilize phosphorous, it is to be converted to absorbable form. Here, the part rendered by phosphate solubilizing bacteria is significant for it plays a crucial role in the formation of plant usable phosphate from organic forms. In the present work, an effort had been made to isolate and identify phosphate solubilising bacterial isolate from the rhizhospheric soils of various plants in Ponthenpuzha forest. One of the isolate from Cymbopogon citrates responded positively to Pikovskaya’s medium by producing a halo zone during in vitro culture. Colony features and 16S rRNA sequence analysis identified the isolate as Burkholderia sps. We have reported the presence of genus Burkholderia in the rhizospheric zone of Cymbopogon citratus. Further studies are warranted for species level identification of the isolate.

scholarly journals Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

2017 ◽  
Vol 66 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jian Zhang ◽  
Peng Cheng Wang ◽  
Ling Fang ◽  
Qi-An Zhang ◽  
Cong Sheng Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Wood Flour ◽  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Sequence Comparisons ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

Isolation and Characterization of a Phosphate-Solubilizing Bacterium Pseudochrobactrum sp. PSB13 from Petroleum-Contaminated Drill Cuttings

2014 ◽  
Vol 955-959 ◽  
pp. 407-410
Author(s):  
Li Bin Zhao ◽  
Xin Xin Wang ◽  
Chen Li ◽  
Yu Chen ◽  
Wei An ◽  
...  
Keyword(s):  
Drilling Process ◽  
Phosphate Solubilizing Bacteria ◽  
Phenotypic Characteristics ◽  
Drill Cuttings ◽  
16S Rdna Sequence ◽  
Isolation And Characterization ◽  
Bacterium Strain ◽  
Phosphate Solubilizing ◽  
First Time

Phosphate-solubilizing bacteria were extensively studied in many environment. However, little is known about them in drill cuttings, as wastes from drilling process. A phosphate-solubilizing bacterium strain PSB13 was isolated from petroleum-contaminated drill cuttings. This strain was identified asPseudochrobactrumsp. based on its 16S rDNA sequence and phenotypic characteristics. This strain could solubilize 97.6 μg/ml phosphates in 6 days when grown in NBRIP liquid medium. The increase in solubilization of phosphate coincided with the drop in pH, which indicates organic acid was responsible for the phosphate-solubilization. Phosphate-solubilizing bacterium was reported in drill cuttings for the first time, which suggests its potential in the bioremediation of petroleum-contaminated drill cuttings.

Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization

2011 ◽  
Vol 47 (4) ◽  
pp. 437-446 ◽  
Author(s):  
Xuan Yu ◽  
Xu Liu ◽  
Tian Hui Zhu ◽  
Guang Hai Liu ◽  
Cui Mao
Keyword(s):  
Phosphate Solubilizing Bacteria ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing ◽  
Effect On Growth

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from corn rhizosfer

2021 ◽  
Vol 911 (1) ◽  
pp. 012063
Author(s):  
Haswania ◽  
H Karim ◽  
A.A. Azis ◽  
N Iriany ◽  
O Jumadi
Keyword(s):  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Methyl Red ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Sucrose Fermentation ◽  
Phosphate Solubilizing ◽  
Sample Dilution

Abstract The aim of this study was to isolate and characterize the Phosphate solubilizing bacteria from the rhizosphere of Zea mays L., Jeneponto Regency. This research was conducted in several stages; i.e, sampling, medium preparation, sample dilution, isolation, characterization in the form of gram staining, biochemical tests, and quantitative tests of phosphate solubility. Soil samples were diluted in 0.9% NaCl and soil containing microbes was isolated on the Picovskaya medium. Three isolates were obtained which could dissolve phosphate, namely J2KN1, J3KR2, and J3TG3 isolates. The isolates were generally round in shape with raised elevations, white, slimy, smooth, shiny surface, milky white, shape like coccus and bacillus, and gram-negative. Some of the isolates had positive motility, indole, voges, methyl red, glucose, and sucrose fermentation in the biochemical test. The quantitative tests of the ability to dissolve phosphate showed that J2KN1 isolate had the highest concentration of 51.1 μM, and the J3KR1 and J3TG3 isolates had a concentration of 45.2 μM and 37.6 μM, respectively.

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