Molecular Cloning of a Partial cDNA Clone Encoding the C Terminal Region of Chicken Breast Muscle Connectin

1996 ◽  
Vol 13 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Hirohiko Yajima ◽  
Hiroshi Ohtsuka ◽  
Hideaki Kume ◽  
Takeshi Endo ◽  
Kei Maruyama ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Cdna Clone ◽  
Terminal Region ◽  
Breast Muscle ◽  
Chicken Breast ◽  
Partial Cdna ◽  
Partial Cdna Clone

Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate

1984 ◽  
Vol 4 (9) ◽  
pp. 1935-1938
Author(s):  
H G Tomasiewicz ◽  
R Cook-Deegan ◽  
D M Chikaraishi
Keyword(s):  
Cdna Clone ◽  
Chicken Embryo ◽  
Kinase Activity ◽  
Chicken Embryo Fibroblast ◽  
Src Kinase ◽  
Cell Protein ◽  
Fibroblast Cells ◽  
Chicken Cell ◽  
Partial Cdna ◽  
Partial Cdna Clone

We have isolated a partial cDNA clone containing sequences complementary to a mRNA encoding a 34- to 36-kilodalton normal chicken cell protein which is a substrate for pp60v-src kinase activity. Using this 34-kilodalton cDNA clone as a probe, we determined that the size of the 34-kilodalton mRNA was 1,100 nucleotides and the level of the 34-kilodalton RNA was the same in various tissues of mature chickens but was significantly higher in chicken embryo fibroblast cells.

scholarly journals Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate.

1984 ◽  
Vol 4 (9) ◽  
pp. 1935-1938 ◽  
Author(s):  
H G Tomasiewicz ◽  
R Cook-Deegan ◽  
D M Chikaraishi
Keyword(s):  
Cdna Clone ◽  
Chicken Embryo ◽  
Kinase Activity ◽  
Chicken Embryo Fibroblast ◽  
Src Kinase ◽  
Cell Protein ◽  
Fibroblast Cells ◽  
Chicken Cell ◽  
Partial Cdna ◽  
Partial Cdna Clone

We have isolated a partial cDNA clone containing sequences complementary to a mRNA encoding a 34- to 36-kilodalton normal chicken cell protein which is a substrate for pp60v-src kinase activity. Using this 34-kilodalton cDNA clone as a probe, we determined that the size of the 34-kilodalton mRNA was 1,100 nucleotides and the level of the 34-kilodalton RNA was the same in various tissues of mature chickens but was significantly higher in chicken embryo fibroblast cells.

scholarly journals A partial cDNA clone for human apolipoprotein B.

1985 ◽  
Vol 82 (15) ◽  
pp. 4983-4986 ◽  
Author(s):  
S. S. Deeb ◽  
A. G. Motulsky ◽  
J. J. Albers
Keyword(s):  
Cdna Clone ◽  
Apolipoprotein B ◽  
Human Apolipoprotein ◽  
Partial Cdna ◽  
Partial Cdna Clone

A Tobacco Ornithine Decarboxylase Partial cDNA Clone

1996 ◽  
Vol 5 (2) ◽  
pp. 109-112 ◽  
Author(s):  
Ved Malik ◽  
Mark B. Watson ◽  
Russell L. Malmberg
Keyword(s):  
Ornithine Decarboxylase ◽  
Cdna Clone ◽  
Partial Cdna ◽  
Partial Cdna Clone

Isolation of juvenile hormone esterase and its partial cDNA clone from the beetle, Tenebrio molitor

2000 ◽  
Vol 30 (7) ◽  
pp. 529-540 ◽  
Author(s):  
Beth Ann Thomas ◽  
Andrew C Hinton ◽  
Haim Moskowitz ◽  
Tonya F Severson ◽  
Bruce D Hammock
Keyword(s):  
Juvenile Hormone ◽  
Cdna Clone ◽  
Tenebrio Molitor ◽  
Juvenile Hormone Esterase ◽  
Partial Cdna ◽  
Partial Cdna Clone

scholarly journals Stage-specific keratins in Xenopus laevis embryos and tadpoles: the XK81 gene family.

1986 ◽  
Vol 103 (5) ◽  
pp. 1957-1965 ◽  
Author(s):  
S Miyatani ◽  
J A Winkles ◽  
T D Sargent ◽  
I B Dawid
Keyword(s):  
Xenopus Laevis ◽  
Cdna Clone ◽  
Cdna Clones ◽  
Type I ◽  
Larval Stages ◽  
Hybridization Data ◽  
Isolation And Characterization ◽  
Partial Cdna ◽  
Flanking Sequences ◽  
Partial Cdna Clone

This report describes the isolation and characterization of genomic and cDNA clones which define a subfamily of type I keratins in Xenopus laevis whose expression is restricted to embryonic and larval stages. The XK81 subfamily, named after the prototype cDNA clone DG81, contains four members arranged in two pairs of closely homologous loci; they were named 81A1, A2, B1, and B2. Genomic clones were obtained representing all of these regions. The A1 gene has been completely sequenced together with approximately 1 kb of flanking sequences at each end; this gene corresponds to the previously reported cDNA clone 8128 (Jonas, E., T. D. Sargent, and I. B. Dawid, 1985, Proc. Natl. Acad. Sci. USA, 82:5413-5417). The B2 gene is represented by a partial cDNA clone, DG118. Upstream sequences and about half of the coding regions have been sequenced for the B1 and B2 genes, whereas the A2 locus has been identified on the basis of hybridization data and could be a gene or pseudogene. Genomic Southern blotting indicates that all members of the subfamily have been isolated. The keratin proteins encoded by the B1 and B2 genes are 96% homologous in the central rod domain, whereas A/B gene homology in this region is 81%. During development mRNAs derived from A and B genes accumulate coordinately during gastrula and neurula stages; in the tadpole, 81A mRNA decays rapidly, whereas 81B mRNA shows a second abundance peak, persists for most of tadpole life, and decays by metamorphosis. RNAs derived from the XK81 keratin subfamily are undetectable in the adult, where different type I keratin genes are expressed.

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