scholarly journals A Highly Sensitive and Rapid Assay for Protein Determination in Human Urine and Penicillin Using a Rayleigh Light-Scattering Technique with Benzeneazo-8-acetylamino-1-naphthol-3,6-disulfonic Acid Sodium Salt

2006 ◽  
Vol 22 (2) ◽  
pp. 275-279 ◽  
Author(s):  
Qin WEI ◽  
Dan WU ◽  
Yan LI ◽  
Bin DU ◽  
Jun Hong WANG
Keyword(s):  
Light Scattering ◽  
Sodium Salt ◽  
Disulfonic Acid ◽  
Protein Determination ◽  
Rayleigh Light Scattering ◽  
Acid Sodium Salt ◽  
Highly Sensitive ◽  
Scattering Technique ◽  
Light Scattering Technique ◽  
Rayleigh Light

scholarly journals Determination of Protein in Milk Powder Using 2-Sulfophenylazo-Rhodanine as a Probe by the Enhanced Resonance Rayleigh Light-Scattering Technique

2006 ◽  
Vol 89 (5) ◽  
pp. 1353-1359
Author(s):  
Qin Wei ◽  
Yan Li ◽  
Wenying Dong ◽  
Bin Du
Keyword(s):  
Light Scattering ◽  
Serum Albumin ◽  
Milk Powder ◽  
Buffer Solution ◽  
Protein Determination ◽  
Rayleigh Light Scattering ◽  
Scattering Technique ◽  
Light Scattering Technique ◽  
Rayleigh Light

Abstract In this paper, the interaction between 2-sulfophenylazo-rhodanine and protein was investigated by Rayleigh light-scattering technique. Based on this, a novel method for the determination of protein was developed. The effects of different conditions, such as acidity and media, were investigated thoroughly, and the optimum conditions were confirmed. Bis(2-ethylhexyl)sulfosuccinate (AOT) microemulsion, which is introduced into the protein determination, markedly increased the sensitivity of the system by changing the microenvironment. In pH 2.80 Britton-Robinson buffer solution in the presence of AOT microemulsion, the detection limits of bovine serum albumin, human serum albumin, ovalbumin, and -globulin are 5.4, 4.5, 9.8, and 10.1 ng/mL, respectively. The method developed in this paper has been applied to the determination of protein in milk powder with satisfactory results.

Study of the interaction of protein and dibromomethyl–Arsenazo–Al(III) by Rayleigh light-scattering technique

2001 ◽  
Vol 442 (2) ◽  
pp. 249-256 ◽  
Author(s):  
Runping Jia ◽  
Lijun Dong ◽  
Qianfeng Li ◽  
Xingguo Chen ◽  
Zhide Hu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Rayleigh Light Scattering ◽  
Scattering Technique ◽  
Light Scattering Technique ◽  
Rayleigh Light
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