scholarly journals Simultaneous determination of homovanillic acid, vanillylmandelic acid and 5-hydroxyindole-3-acetic acid in urine by high performance liquid chromatography.

1984 ◽  
Vol 33 (7) ◽  
pp. E257-E262 ◽  
Author(s):  
Akira YOSHIDA ◽  
Yuko YAMAGUCHI ◽  
Masanori YOSHIOKA ◽  
Zenzo TAMURA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Homovanillic Acid ◽  
Vanillylmandelic Acid

Determination of vanillylmandelic acid, vanillactic acid, and homovanillic acid in dried urine on filter-paper discs by high-performance liquid chromatography with coulometric electrochemical detection for neuroblastoma screening.

1988 ◽  
Vol 34 (11) ◽  
pp. 2228-2230 ◽  
Author(s):  
Y Kinoshita ◽  
S Yamada ◽  
K Haraguchi ◽  
T Takayanagi ◽  
Y Mori ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
Filter Paper ◽  
High Performance ◽  
Homovanillic Acid ◽  
Vanillylmandelic Acid ◽  
Chromatographic Procedure ◽  
One Year

Abstract We report a method for determination of vanillylmandelic acid, vanillactic acid, and homovanillic acid by high-performance liquid chromatography (HPLC), with coulometric electrochemical detection, for mass screening of neuroblastoma. Urine samples were collected on filter paper, dried, and then pretreated. The chromatographic procedure is reliable and fast, allowing for a large sample throughput for routine screening. Intricate extraction procedures and centrifugal separation are unnecessary. Screening for neuroblastoma by HPLC is rapidly gaining acceptance in Japan, and our method is being used at many screening centers. Of 26,571 infants screened in one year in Yokohama City, our method detected five with neuroblastoma.

Simultaneous determination of acidic 3,4-dihydroxyphenylalanine metabolites and 5-hydroxyindole-3-acetic acid in urine by high-performance liquid chromatography

1990 ◽  
Vol 36 (10) ◽  
pp. 1834-1837 ◽  
Author(s):  
A E Stroomer ◽  
H Overmars ◽  
N G Abeling ◽  
A H van Gennip
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Reversed Phase ◽  
Fluorescence Detector ◽  
Gas Chromatographic Method ◽  
High Degree

Abstract We describe a simple and rapid quantitative method for the simultaneous determination of 3,4-dihydroxyphenylalanine acid metabolites and 5-hydroxyindole-3-acetic acid. After solvent extraction from acidified urine, the acids are analyzed by reversed-phase high-performance liquid chromatography. For detection and quantification, we used a fluorescence detector in combination with an amperometric detector to obtain a high degree of specificity. Sample preparation and chromatographic analysis can be completed within an hour. Results by the method correlate well with those by a previously used gas-chromatographic method, but the new method is faster and avoids the need for derivatization.

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