A simple high-performance liquid chromatography assay for simultaneous determination of plasma norepinephrine, epinephrine, dopamine and 3,4-dihydroxyphenyl acetic acid

1999 ◽  
Vol 21 (3) ◽  
pp. 519-525 ◽  
Author(s):  
Yushan Wang ◽  
Debra S Fice ◽  
Pollen K.F Yeung
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Plasma Norepinephrine

Simultaneous determination of acidic 3,4-dihydroxyphenylalanine metabolites and 5-hydroxyindole-3-acetic acid in urine by high-performance liquid chromatography

1990 ◽  
Vol 36 (10) ◽  
pp. 1834-1837 ◽  
Author(s):  
A E Stroomer ◽  
H Overmars ◽  
N G Abeling ◽  
A H van Gennip
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Reversed Phase ◽  
Fluorescence Detector ◽  
Gas Chromatographic Method ◽  
High Degree

Abstract We describe a simple and rapid quantitative method for the simultaneous determination of 3,4-dihydroxyphenylalanine acid metabolites and 5-hydroxyindole-3-acetic acid. After solvent extraction from acidified urine, the acids are analyzed by reversed-phase high-performance liquid chromatography. For detection and quantification, we used a fluorescence detector in combination with an amperometric detector to obtain a high degree of specificity. Sample preparation and chromatographic analysis can be completed within an hour. Results by the method correlate well with those by a previously used gas-chromatographic method, but the new method is faster and avoids the need for derivatization.

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