scholarly journals Allium Sativum Leaf Agglutinin (ASAL) Endowed Enhanced Resistance Against Myzus Persicae under Both Constitutive and Phloem Specific Promoters

2021 ◽  
Author(s):  
Noroza Umer ◽  
Rubab Zahra Naqvi ◽  
Imran Rauf ◽  
Naveed Anjum ◽  
Hamid Anees Siddiqui ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Myzus Persicae ◽  
Plant Viruses ◽  
Transgenic Tobacco Plants ◽  
Specific Primers ◽  
Tobacco Plants ◽  
Aphid Infestation ◽  
Variable Expression ◽  
Enhanced Resistance ◽  
Plant Transformation Vector

Abstract Globally, aphid, Myzus persicae is an economically significant, polyphagous crop pest that feeds on more than 400 plant species and transmits more than 100 plant viruses. Aphid infestation is mostly managed by insecticides that cause heavy environmental contamination and insect resistance. Cloning of plant derived insecticidal genes to develop transgenic plants under suitable promoter is a promising technology. In the present study, ASAL (MN820725) was isolated from native garlic and cloned in plant transformation vector, pGA482 through Agrobacterium mediated tobacco transformation. PCR of genomic DNA of transgenic tobacco plants using gene specific primers confirmed the presence of asal gene of 546 bp. To detect the integration of gene Southern blot analysis was conducted that revealed stable integration of asal gene while, gene expression was analyzed through qRT-PCR that showed variable expression of asal gene in transgenic tobacco plants. Efficacy of asal gene was evaluated through aphid bioassay. Aphid bioassay revealed that transgenic tobacco lines LS-17, LS-20, LR-1, and LR-7 exhibited 100% aphid mortality and significantly reduced the aphid population. These findings suggested the potential of ASAL against aphids that can be further used against other notorious sap sucking pests.

Transgenic tobacco expressing an Arisaema heterophyllum agglutinin gene displays enhanced resistance to aphids

2004 ◽  
Vol 84 (3) ◽  
pp. 785-790 ◽  
Author(s):  
Jianhong Yao, Xiuyun Zhao ◽  
Huaxiong Qi, Bingliang Wan ◽  
Fei Chen, Xiaofen Sun ◽  
Shanqian Yu ◽  
Kexuan Tang
Keyword(s):  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Myzus Persicae ◽  
Transgenic Tobacco Plants ◽  
Insect Bioassay ◽  
Tobacco Plants ◽  
Potato Aphid ◽  
Enhanced Resistance ◽  
Multiple Copies ◽  
Peach Potato Aphid

Tobacco leaf discs were transformed with a plasmid, pBIAHA, containing the selectable marker neomycin phosphotransferase gene (nptII) and an Arisaema heterophyllum agglutinin gene (aha) via Agrobacterium tumefaciens-mediated transformation. Thirty-two independent transgenic tobacco plants were regenerated. PCR and Southern blot analyses confirmed that multiple copies of the aha gene had integrated into the plant genome. Northern blot analysis revealed that the aha gene was expressed at various levels in the transgenic plants. Insect bioassay test showed that transgenic plants expressing multiple copies of the aha gene reduced the rate of population increase of the peach potato aphid (Myzus persicae Sulzer). This is the first report that transgenic tobacco plants expressing the aha gene display enhanced resistance to aphids. Key words: Insect bioassay, Arisaema heterophyllum agglutinin, transformation, transgenic tobacco, peach potato aphid (Myzus persicae Sulzer)

scholarly journals Role for Salicylic Acid in the Activation of Defense Responses in Catalase-Deficient Transgenic Tobacco

1997 ◽  
Vol 10 (7) ◽  
pp. 922-925 ◽  
Author(s):  
He Du ◽  
Daniel F. Klessig
Keyword(s):  
Salicylic Acid ◽  
Transgenic Tobacco ◽  
Catalase Activity ◽  
Defense Responses ◽  
Transgenic Tobacco Plants ◽  
Strong Light ◽  
Tobacco Plants ◽  
Enhanced Resistance ◽  
The Cross

Transgenic tobacco plants with severely reduced catalase activity were crossed with NahG plants that do not accumulate salicylic acid. Some of the progeny from the cross spontaneously developed necrosis when grown under strong light, similar to that observed on the parental severely catalase-deficient plants. However, in contrast to the parental catalase-deficient plants, these progeny plants did not constitutively express PR-1 genes or develop enhanced resistance. Thus, salicylic acid appears to be required for the induction of these defense responses in catalase-deficient tobacco plants.

scholarly journals Production of santalenes and bergamotene in Nicotiana tabacum plants

2018 ◽  
Author(s):  
Jun-Lin Yin ◽  
Woon-Seng Wong
Keyword(s):  
Transgenic Plants ◽  
Pest Management ◽  
Transgenic Tobacco ◽  
Green Peach Aphid ◽  
Mevalonic Acid ◽  
Transgenic Tobacco Plants ◽  
Trap Crop ◽  
Chloroplast Targeting ◽  
Tobacco Plants ◽  
Aphid Infestation

AbstractTerpenes play an important role in plant–insect relationships, and these relationships can potentially be modified by altering the profile of terpenes emitted from plants using metabolic engineering methods. Transgenic plants generated by employing such methods offer the prospect of low-cost sustainable pest management; in this regard, we used chloroplast targeting and cytosolic mevalonic acid pathway enhancement in this study to investigate the interaction of santalenes and bergamotene with insects. The santalene- and bergamotene-emitting transgenic tobacco plants thus generated were utilized to study host preference in the green peach aphid Myzus persicae (Sulzer). The results showed that co-expression of either 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) or truncated HMGR with santalene synthase led to the production of higher amounts of santalenes and bergamotene in transgenic tobacco plants, and that these santalene- and bergamotene-emitting plants were attractive to green peach aphids. We accordingly propose that such transgenic plants may have potential application in pest management as a trap crop to prevent green peach aphid infestation of wild-type tobacco plants.

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