scholarly journals Evaluation and Diagnosis of Nonalcoholic Fatty Liver Disease/Steatohepatitis by K-Fold Validation Based on Pathology, Including Real-Time Shear Wave Elastography and Noninvasive Biomarkers

2020 ◽  
Author(s):  
Jie Zhou ◽  
Feng Yan ◽  
Jinshun Xu ◽  
Qiang Lu ◽  
Xianglan Zhu ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Shear Wave ◽  
Fatty Liver Disease ◽  
Liver Stiffness ◽  
Shear Wave Elastography ◽  
Nonalcoholic Fatty Liver ◽  
Noninvasive Biomarkers ◽  
Diagnostic Indicator

Abstract ObjectivesThis study aim to investigate the diagnostic accuracy of shear wave elastography (SWE) for diagnosing nonalcoholic steatohepatitis (NASH) and staging fibrosis in a cohort patients confirmed nonalcoholic fatty liver disease (NAFLD) by liver biopsy.MethodsA total of 86 NAFLD patients and 17 normal-control were enrolled. The performance of SWE to diagnose NASH and stage fibrosis was evaluated on the basis of histopathological inflammation grades and fibrosis stages according to Kleiner/Brunt et al.’s criteria classification, and compared to previous reported four noninvasive serum fibrotic scores, coupled with the k-fold-cross-validation and Delong test. Meanwhile, influence of steatosis on liver stiffness measurements (LSMs) of SWE was also studied.ResultsLSMs of SWE proved to be an excellent diagnostic indicator for detecting NASH (AUROC＝0.85), and fibrotic NASH: ≥F2 stage (AUROC＝0.92), ≥F3 stage (AUROC＝0.94) and =F4 stage (AUROC ＝0.94) with the cutoff values were 7.55, 7.65, 8.25 and 11.80 kPa, respectively. Compared with serum fibrotic scores, SWE had the highest AUROC for predicting ≥F2, ≥F3, =F4 by Delong test (all P＜0.05). No statistic differences of LSMs were found among different steatosis levels (P=0.29).ConclusionThe stiffness reconstructions based on SWE could be used to noninvasively identify NASH and stage fibrosis in NAFLD patients. Moreover, the diagnosis efficiency of LSMs on SWE could not be influenced by steatosis.

scholarly journals Proteomic screening of plasma identifies potential noninvasive biomarkers associated with significant/advanced fibrosis in patients with nonalcoholic fatty liver disease

2020 ◽  
Vol 40 (1) ◽  
Author(s):  
Wei Hou ◽  
Michael G. Janech ◽  
Philip M. Sobolesky ◽  
Alison M. Bland ◽  
Salma Samsuddin ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Fatty Liver Disease ◽  
Liver Stiffness ◽  
Complement Component ◽  
Advanced Fibrosis ◽  
Nonalcoholic Fatty Liver ◽  
Noninvasive Biomarkers ◽  
Complement Component C8

Abstract Noninvasive biomarkers are clinically useful for evaluating liver fibrosis stage in patients with nonalcoholic fatty liver disease (NAFLD). The aim of the present study was to compare plasma proteins in patients with early nonalcoholic steatohepatitis (NASH) (F0-F1) versus NASH with significant/advanced fibrosis (F2–F4) to determine whether candidate proteins could be used as potential noninvasive biomarkers. Nineteen biopsy-proven NAFLD patients including ten early NASH patients and nine NASH patients with significant/advanced fibrosis were enrolled in the present study. High-resolution proteomics screening of plasma was performed with the SCIEX TripleTOF 5600 System. Proteins were quantified using two different software platforms, Progenesis Qi and Scaffold Q+, respectively. Progenesis Qi analysis resulted in the discovery of 277 proteins compared with 235 proteins in Scaffold Q+. Five consensus proteins (i.e. Complement component C7; α-2-macroglobulin; Complement component C8 γ chain; Fibulin-1; α-1-antichymotrypsin) were identified. Complement component C7 was three-fold higher in the NASH group with significant/advanced fibrosis (F2–F4) compared with the early NASH (F0-F1) group (q-value = 3.6E-6). Complement component C7 and Fibulin-1 are positively correlated with liver stiffness (P=0.000, P=0.002, respectively); whereas, Complement component C8 γ chain is negatively correlated (P=0.009). High levels of Complement C7 are associated with NASH with significant/advanced fibrosis and Complement C7 is a perfect classifier of patients included in this pilot study. Further studies will be needed in a larger validation cohort to confirm the utility of complement proteins as biomarkers or mechanistic determinants of NASH with significant/advanced fibrosis.

scholarly journals Elevated Th22 cells correlated with Th17 cells in patients with high liver stiffness in nonalcoholic fatty liver disease

2018 ◽  
Vol 16 ◽  
pp. 205873921880267 ◽  
Author(s):  
Si-Biao Su ◽  
Wen Chen ◽  
Fei-Fei Huang ◽  
Jian-Feng Zhang
Keyword(s):  
Flow Cytometry ◽  
Liver Disease ◽  
Plasma Level ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Fatty Liver Disease ◽  
Th17 Cells ◽  
Liver Stiffness ◽  
Nonalcoholic Fatty Liver ◽  
Th22 Cells

The aims of this article are to investigate the effects of Th22 and Th17 cells and plasma cytokines in patients with nonalcoholic fatty liver disease (NAFLD) and to examine the correlation between Th22 and Th17 cells levels and disease progression. Blood samples from 70 patients with NAFLD and 26 healthy controls (HCs) were collected. Flow cytometry and enzyme-linked immunosorbent assay (ELISA) analysis were performed. ELISA revealed interleukin (IL)-22 plasma level in FibroScan subgroup patients was 2.2-fold higher than that in the HCs ( P < 0.01), body mass index (BMI) subgroup patients were 2.3-fold higher than HCs ( P < 0.01), IL-17A plasma level was 5.6-fold higher in FibroScan subgroup patients than that in the HCs ( P < 0.01), and 5.8-fold higher in BMI subgroup than HCs ( P < 0.01). Flow cytometry revealed median proportions of Th22 cells in FibroScan group was greater than that in the HCs (3.85% vs 0.86%; P < 0.001). Similar results were obtained for Th17 cells (6.36% vs 0.9%; P < 0.001). The liver stiffness measurement value was highly correlated with the proportion of Th17 and Th22 cells and moderately correlated with the plasma level of IL-17A, IL-22, and the BMI value. In conclusion, the elevated proportions of Th17 and Th22 cells were significantly correlated with the degree of liver cirrhosis in NAFLD patients. They may play an inflammatory and immune response role in progression from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH) or fibrosis.

Influence of liver stiffness heterogeneity on staging fibrosis in patients with nonalcoholic fatty liver disease

Hepatology ◽  
2021 ◽  
Author(s):  
Nobuyoshi Kawamura ◽  
Kento Imajo ◽  
Kyle J. Kalutkiewicz ◽  
Koki Nagai ◽  
Michihiro Iwaki ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Fatty Liver Disease ◽  
Liver Stiffness ◽  
Nonalcoholic Fatty Liver

scholarly journals Liver Stiffness in Pediatric Patients with Fatty Liver Disease: Diagnostic Accuracy and Reproducibility of Shear-Wave Elastography

Radiology ◽  
2017 ◽  
Vol 283 (3) ◽  
pp. 820-827 ◽  
Author(s):  
Matteo Garcovich ◽  
Silvio Veraldi ◽  
Enrico Di Stasio ◽  
Maria Assunta Zocco ◽  
Lidia Monti ◽  
...  
Keyword(s):  
Liver Disease ◽  
Diagnostic Accuracy ◽  
Fatty Liver ◽  
Shear Wave ◽  
Fatty Liver Disease ◽  
Pediatric Patients ◽  
Liver Stiffness ◽  
Shear Wave Elastography

scholarly journals S1024 Evaluation of the FAST Score in Patients With Nonalcoholic Fatty Liver Disease and High Liver Stiffness Measurements

2020 ◽  
Vol 115 (1) ◽  
pp. S522-S522
Author(s):  
Pankaj Aggarwal ◽  
Allison Harrington ◽  
Tamneet Singh ◽  
Oladuni Cummings-John ◽  
Anita Kohli ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Fatty Liver Disease ◽  
Liver Stiffness ◽  
Nonalcoholic Fatty Liver ◽  
High Liver
Sign in / Sign up

Export Citation Format

Share Document