scholarly journals The Promoting Effects of Activated Olfactory Ensheathing Cells on Angiogenesis after Spinal Cord Injury Through the PI3K/Akt Pathway

2022 ◽  
Author(s):  
Xiaohui Wang ◽  
Chao Jiang ◽  
Yongyuan Zhang ◽  
Zhe Chen ◽  
Hong Fan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Endothelial Cells ◽  
Olfactory Ensheathing Cells ◽  
Akt Pathway ◽  
Angiogenic Growth Factors ◽  
Angiogenic Potential ◽  
Positive Effects ◽  
Cord Injury ◽  
Ensheathing Cells

Abstract ObjectiveThe aim of this study was to investigate the pro-angiogenic potential of olfactory ensheathing cells (OECs) activated by curcumin (CCM) and lipopolysaccharide (LPS) and the possible underlying mechanisms. MethodsVascular endothelial cells or tissues were cultured and treated with conditioned medium (CM) extracted from the activated through the addition of LPS and CCM or unactivated OECs. Concomitantly, the pro-angiogenic potential of OECs was assessed in vitro by aortic ring sprouting assay, endothelial wound healing assay, CCK-8 assay and tube formation assay. Subsequently, the OECs were co-cultured with endothelial cells to evaluate their promoting effect on the proliferation and migration of endothelial cells following undergoing a mechanical scratch. Moreover, the spinal cord injury (SCI) model in rats was established, and the number of endothelial cells and vascular structure in the injured area after SCI was observed with OECs transplantation. Finally, the underlying mechanism was investigated by western blot analysis of phosphorylated kinase expression with or without the MK-2206 (Akt-inhibitor). ResultThe present results showed that the activated OECs can effectively promote the proliferation, migration and vessel-like structure formation of vascular endothelial cells. Strikingly, several pro-angiogenic growth factors such as VEGF-A and PDGF-AA, which facilitate vessel formation, were found to be significantly elevated in CM. In addition, the PI3K/Akt signaling pathway involved in pro-angiogenic event caused by activated OEC CM, displaying higher phosphorylation levels in cells. On contrary, the delivery of MK2206 can effectively abrogate all the positive effects. ConclusionsOECs activated by LPS and CCM, have a strong pro-angiogenesis effect, and can effectively promote angiogenesis and improve the injury microenvironment when transplanted in injured spinal cord. This potentiated ability of OECs to pro-angiogenesis is likely mediated through the PI3K/Akt pathway.

scholarly journals Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury

2017 ◽  
Vol 40 (10) ◽  
pp. 1630-1637 ◽  
Author(s):  
Yin-Yao Tang ◽  
Wei-Xiao Guo ◽  
Zheng-Feng Lu ◽  
Mao-Hua Cheng ◽  
Yi-Xin Shen ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Olfactory Ensheathing Cells ◽  
Akt Pathway ◽  
Ginsenoside Rg1 ◽  
Rat Spinal Cord ◽  
Cord Injury ◽  
Ensheathing Cells

scholarly journals Comparison of intramedullary transplantation of olfactory ensheathing cell for patients with chronic complete spinal cord injury worldwide

2018 ◽  
Vol 1 (1) ◽  
pp. 146-151 ◽  
Author(s):  
Lin Chen ◽  
Yuqi Zhang ◽  
Xijing He ◽  
Saberi Hooshang
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Transplantation ◽  
Clinical Results ◽  
Postoperative Management ◽  
Olfactory Ensheathing Cells ◽  
Olfactory Ensheathing Cell ◽  
Advantages And Disadvantages ◽  
Cord Injury ◽  
Ensheathing Cells

Objectives:Traumatic spinal cord injury (tSCI) remains a major clinical challenge. Cell transplantation brings a glimmer of light, among them olfactory ensheathing cells (OECs) have shown some neurorestorative effect. Due to the results of each group lack basic consistency, many technical details are believed to affect the overall outcome. We compare the clinical outcome of intramedullary transplant of olfactory ensheathing cells for patients with spinal cord injury at multi-centers worldwide, and to explore the potential standardized transplantation that suits for the clinical requirements.Methods:Here, we used the Pubmed and CNKI databases to search online the literatures published in the last 20 years for the clinical studies/trials of OECs for chronic spinal cord injury in the representative clinical center. The results of these representative clinical treatment centers were searched and analyzed. The parameters which may affect the effect including the concentration of cells, the total number of cells, the choice of incision, the site of transplantation, the number of transplantation sites, the advantages and disadvantages of transplantation equipment, and postoperative management, were compared carefully to clarify its impact on the clinical results.Results:In these literatures, 2 Chinese centers, 1 Australian center and 1 European center were selected for intraspinal transplantation. The reason of different results may be due to the excessive injection times and/or the excessive total injection volume.Conclusions:Cell implant to the spinal cord parenchyma is effective for restoring neurological functions, but improper procedures may lead to ineffective results. Concise surgery appears to be more suitable for clinical application than ostensibly precise and complex injection procedures. Sufficient rehabilitation training is surely necessary for the integration of motor recovery after cell transplantation.

Inhibition of ADAMTS-4 Expression in Olfactory Ensheathing Cells Enhances Recovery after Transplantation within Spinal Cord Injury

2020 ◽  
Vol 37 (3) ◽  
pp. 507-516
Author(s):  
Quentin Delarue ◽  
Anne Mayeur ◽  
Chaima Chalfouh ◽  
Axel Honoré ◽  
Célia Duclos ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Olfactory Ensheathing Cells ◽  
Cord Injury ◽  
Ensheathing Cells
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