scholarly journals Rubber Degrading Strains of Microtetraspora and Dactylosporangium

2021 ◽  
Author(s):  
Ann Anni Basik ◽  
Nanthini Jayaram ◽  
Chia Tiong Yeo ◽  
Kumar Sudesh
Keyword(s):  
16S Rdna ◽  
Gene Sequence ◽  
Oxidative Cleavage ◽  
Operon Structure ◽  
Spectrophotometric Detection ◽  
16S Rdna Gene ◽  
Rdna Gene

Abstract Latex clearing protein (lcp) found in Actinobacterial strains is reportedly critical for the initial oxidative cleavage of poly(cis-1,4-isoprene), the major polymeric unit of rubber. In this study, we screened 940 Actinobacterial strains isolated from various locations in Sarawak on NR latex agar and identified 18 strains from 5 genera that produced clearing zones and contained (latex clearing protein) lcp genes. We report here the first lcp genes from Microtetraspora sp. AC03309 (lcp 1 and lcp2) and Dactylosporangium sp. AC04546 (lcp1, lcp2, lcp3), together with their operon structure. Complete 16S rDNA gene sequence revealed that Dactylosporangium sp. AC04546 is 99% identical to Dactylosporangium sucinum RY35-23 whereas Microtetraspora sp. AC03309 is 98% identical to Microtetraspora glauca IFO14761. Morphological images and the spectrophotometric detection of aldehyde and keto groups in rubber samples incubated with the strains confirm the strains’ ability to degrade rubber-based products.

Removal Characteristics of Microcystis aeruginosa with One Brevibacillus parabrevis Strain

2010 ◽  
Vol 113-116 ◽  
pp. 928-931
Author(s):  
Rui Min Mu ◽  
Xue Liang Yuan ◽  
Gui Xia Ma
Keyword(s):  
16S Rdna ◽  
Microcystis Aeruginosa ◽  
Removal Efficiency ◽  
Water Body ◽  
Gene Sequence ◽  
Physiological Characteristics ◽  
16S Rdna Gene ◽  
Rdna Gene ◽  
Algicidal Bacterium ◽  
Brevibacillus Parabrevis

In this study, one strain of algicidal bacterium H01 was selected from the water body polluted by Microcystis aeruginosa. It had good removal effects on Microcystis aeruginosa and the removal characteristics on Microcystis aeruginosa were studied. The results indicated that the removal efficiency was connected with such factors as temperature and pH. And the experiment on algicidal mechanism indicated that it lysed the algae cells by secreting metabolites. According to the analysis of its physiological characteristics and 16S rDNA gene sequence, the strain H01 was identified as Brevibacillus parabrevis.

scholarly journals Isolation and Identification of Actinobacteria from Surface-Sterilized Wheat Roots

2003 ◽  
Vol 69 (9) ◽  
pp. 5603-5608 ◽  
Author(s):  
Justin T. Coombs ◽  
Christopher M. M. Franco
Keyword(s):  
16S Rdna ◽  
Gene Sequence ◽  
South Australia ◽  
Isolation And Identification ◽  
16S Rdna Gene ◽  
Wheat Roots ◽  
Rdna Gene ◽  
Plant Microbe Interaction ◽  
Rdna Sequencing ◽  
Root Tissues

ABSTRACT This is the first report of filamentous actinobacteria isolated from surface-sterilized root tissues of healthy wheat plants (Triticum aestivum L.). Wheat roots from a range of sites across South Australia were used as the source material for the isolation of the endophytic actinobacteria. Roots were surface-sterilized by using ethanol and sodium hypochlorite prior to the isolation of the actinobacteria. Forty-nine of these isolates were identified by using 16S ribosomal DNA (rDNA) sequencing and found to belong to a small group of actinobacterial genera including Streptomyces, Microbispora, Micromonospora, and Nocardiodes spp. Many of the Streptomyces spp. were found to be similar, on the basis of their 16S rDNA gene sequence, to Streptomyces spp. that had been isolated from potato scabs. In particular, several isolates exhibited high 16S rDNA gene sequence homology to Streptomyces caviscabies and S. setonii. None of these isolates, nor the S. caviscabies and S. setonii type strains, were found to carry the nec1 pathogenicity-associated gene or to produce the toxin thaxtomin, indicating that they were nonpathogenic. These isolates were recovered from healthy plants over a range of geographically and temporally isolated sampling events and constitute an important plant-microbe interaction.

scholarly journals Cultivable bacterial diversity associated with bromeliad roots from ironstone outcrops in central Brazil

2020 ◽  
Vol 80 (4) ◽  
pp. 872-880
Author(s):  
T. F. C. Viana ◽  
A. P. S. Campelo ◽  
J. I. Baldani ◽  
P. I. Fernandes-Júnior ◽  
V. L. D. Baldani ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
16S Rdna ◽  
Endophytic Bacteria ◽  
Gene Sequence ◽  
Community Diversity ◽  
Intraspecific Diversity ◽  
Wild Plants ◽  
16S Rdna Gene ◽  
Mato Grosso ◽  
Rdna Gene

Abstract Studies on the bacterial diversity associated with wild plants are rare, especially on those that grow in association with bromeliads. In the present study, we isolated and identified epiphytic and endophytic bacteria from the roots of the bromeliads Dyckia excelsa, Dyckia leptostachya and Deuterocohnia meziana occurring in the “cangas” in the Pantanal from Mato Grosso do Sul State, Brazil. The epiphytic bacteria were isolated from washed roots, while the endophytic bacteria were isolated from surface disinfested roots. Bacterial representatives corresponding to each BOX-PCR fingerprint, as well as those that did not result in amplicons, were selected for 16S rDNA gene sequence analysis. The BOX-PCR data showed intrageneric and intraspecific diversity and could discriminate strains and identify their phenotypic characteristics. The 16S rDNA gene sequence and phylogeny analysis showed a higher occurrence of strains belonging to the genus Bacillus than Mycobacterium and Brevibacterium, which were found in lower numbers. Species from the Bacillus genus are well known for their sporulation capacity and longer survival in arid locations, such as the “cangas”. This study clearly showed that the bromeliad species represent a vast reservoir of bacterial community diversity, and the cultivable strains represent a new source for biotechnological prospecting.

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