scholarly journals Detection and difference analysis of the enzyme activity of colloidal gold nanoparticles with negatively charged surfaces prepared by different reducing agents

2021 ◽  
Author(s):  
Junjun Cao ◽  
Jie Zhang ◽  
Ning Yuan ◽  
Yixiao Sun ◽  
Zhihua Xu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Enzyme Activity ◽  
Hydroxyl Radical ◽  
Colloidal Gold ◽  
Negative Charge ◽  
Linear Chain ◽  
Reducing Agents ◽  
Difference Analysis ◽  
Charged Surfaces ◽  
Colloidal Gold Nanoparticles

Abstract Research on the activity of nanoenzymes has always been a focus of nanomaterials. In this study, several reducing agents with different structures were used to prepare colloidal gold with a negative charge and similar size by controlling the temperature and pH. The affinity analysis of the substrate H 2 O 2 and TMB showed that the activity of colloidal gold nanoenzymes prepared by different reducing agents was in the order of Cc, Hq, Rs, Vc, Ga, Sc, Sm, St. The rule is that the enzyme activity of colloidal gold reduced by benzene ring is higher than that of colloidal gold enzyme reduced by linear chain. Finally, we discussed the activity of colloidal gold peroxidase from the number and position of isomers and functional groups, and demonstrated that the nanoenzyme activity affected the surface activity of colloidal gold, the elimination of hydroxyl radical and TMB binding efficiency.

scholarly journals Detection and Difference Analysis of the Enzyme Activity of Colloidal Gold Nanoparticles With Negatively Charged Surfaces Prepared by Different Reducing Agents

2022 ◽  
Vol 9 ◽  
Author(s):  
Mingze Ma ◽  
Junjun Cao ◽  
Ashe Fang ◽  
Zhihua Xu ◽  
Tieying Zhang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Colloidal Gold ◽  
Sodium Citrate ◽  
Negative Charge ◽  
Reducing Agents ◽  
Sodium Tartrate ◽  
Charged Surfaces ◽  
Linear Chains ◽  
Pyrogallic Acid ◽  
Sodium Malate

Nanozymes are particles with diameters in the range of 1–100 nm, which has been widely studied due to their biological enzyme-like properties and stability that natural enzymes do not have. In this study, several reducing agents with different structures (catechol (Cc), hydroquinone (Hq), resorcinol (Rs), vitamin C (Vc), pyrogallic acid (Ga), sodium citrate (Sc), sodium malate (Sm), and sodium tartrate (St)) were used to prepare colloidal gold with a negative charge and similar particle size by controlling the temperature and pH. The affinity analysis of the substrate H2O2 and TMB showed that the order of activities of colloidal gold Nanozymes prepared by different reducing agents was Cc, Hq, Rs, Vc, Ga, Sc, Sm, St. It was also found that the enzyme activity of colloidal gold reduced by benzene rings is higher than that of the colloidal gold enzyme reduced by linear chains. Finally, we discussed the activity of the colloidal gold peroxidase based on the number and position of isomers and functional groups; and demonstrated that the nanozymes activity is affected by the surface activity of colloidal gold, the elimination of hydroxyl radicals and the TMB binding efficiency.

Dense aqueous colloidal gold nanoparticles prepared from highly concentrated precursor solution

2011 ◽  
Vol 362 (2) ◽  
pp. 325-329 ◽  
Author(s):  
Tetsuro Soejima ◽  
Seisaku Oshiro ◽  
Yasuji Nakatsuji ◽  
Seishiro Ito
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Precursor Solution ◽  
Colloidal Gold Nanoparticles

An enhanced ELISA based on modified colloidal gold nanoparticles for the detection of Pb(II)

2011 ◽  
Vol 26 (8) ◽  
pp. 3700-3704 ◽  
Author(s):  
Yu Zhou ◽  
Xiang-Li Tian ◽  
Yan-Song Li ◽  
Feng-Guang Pan ◽  
Yuan-Yuan Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Colloidal Gold Nanoparticles

Effect of Voltage on Colloidal Gold (Au) Nanoparticles Produced Using Electro-Dissolution-Reduction Method

2015 ◽  
Vol 1115 ◽  
pp. 386-389
Author(s):  
Haroon Haiza ◽  
I.I. Yaacob ◽  
Ahmad Zahirani Ahmad Azhar
Keyword(s):  
Gold Nanoparticles ◽  
Electron Microscope ◽  
Colloidal Gold ◽  
Reduction Process ◽  
Chloride Ions ◽  
Complex Ions ◽  
Dc Power ◽  
Colloidal Gold Nanoparticles ◽  
Transmission Electron ◽  
The Mean

Colloidal gold nanoparticles have been successfully prepared using a simple two-electrode cells connected to a DC power supply. During the electro-dissolution-reduction process, the bulk gold at the anode oxidized into gold cations which then reacted with the chloride ions to form aurochloride complex. The complex ions were then reduced by the citrate ion to form colloidal gold nanoparticles. The size and shape of the nanoparticles were modulated by varying the terminal voltages. The colloidal gold nanoparticles obtained were characterized by field-emission scanning electron microscope (FESEM), transmission electron microscope (TEM) and ultraviolet-visible spectrophotometer (UV-Vis). From FESEM analysis, it was found that by increasing the voltage, the size of colloidal gold nanoparticles produced marginally decreased. The mean sizes of gold nanoparticles were roughly about 23.5 nm, 23.2 nm and 19.3 nm for 32 V, 36 V and 40 V, respectively. TEM micrograph showed that the shape of gold nanoparticles obtained is almost spherical. The characteristic peaks of UV-Vis spectra revealed that the suspension was indeed colloidal gold nanoparticles. Keywords: Gold, Nanoparticles, Electro-dissolution-reduction

scholarly journals Back Cover: Unconventional Chain-Growth Mode in the Assembly of Colloidal Gold Nanoparticles (Angew. Chem. Int. Ed. 32/2012)

2012 ◽  
Vol 51 (32) ◽  
pp. 8122-8122 ◽  
Author(s):  
Hong Wang ◽  
Liyong Chen ◽  
Xiaoshuang Shen ◽  
Liangfang Zhu ◽  
Jiating He ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Growth Mode ◽  
Chain Growth ◽  
Back Cover ◽  
Colloidal Gold Nanoparticles

Effect of the size of colloidal gold nanoparticles on the physical parameters of nanocomposite microcapsule shells

2009 ◽  
Vol 71 (3) ◽  
pp. 422-429 ◽  
Author(s):  
A. M. Yashchenok ◽  
O. A. Inozemtseva ◽  
D. A. Gorin ◽  
B. N. Khlebtsov
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Gold ◽  
Physical Parameters ◽  
Colloidal Gold Nanoparticles
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