scholarly journals Obtaining of Zinc Oxide Nanoparticles Modified With Galactose and Assessment of Their Cytotoxic Properties

2021 ◽  
Author(s):  
Jolanta Pulit-Prociak ◽  
Anita Staroń ◽  
Olga Długosz ◽  
Dominik Domagała ◽  
Katarzyna Janczyk ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Living Organism ◽  
Crystallographic Structure ◽  
Oxide Nanoparticles ◽  
Zinc Ions ◽  
Ovary Cells ◽  
Stable Forms

Abstract The work presents the method for preparation of zinc oxide nanoparticles with galactose coating. Bare zinc oxide nanoparticles are well-known and popular drug delivers. However, the fact that zinc ions may be released from their structure easily, they may pose a threat to the living organism. Thus the modification of such a product has been performed. The physicochemical properties of the products have been analysed. XRD technique revealed crystallographic structure of the products. Based on ATR-FTIR analysis it was confirmed that galactose has been successfully attached to the zinc oxide nanoparticles. TEM-EDS microscopy was applied in order to assess the shape of nanoparticles and also for the confirmation of galactose on the particles surface. The releasing of zinc ions from the modified products was compared to their releasing from basic, non-modified sample. Also, cytotoxicity and proliferation of obtained products have been analysed with using Chinese hamster ovary cells. It was found out that the proposed technology may lead to obtain stable forms of modified zinc oxide nanoparticles with limited toxicity.

Autophagy-dependent release of zinc ions is critical for acute lung injury triggered by zinc oxide nanoparticles

2018 ◽  
Vol 12 (9) ◽  
pp. 1068-1091 ◽  
Author(s):  
Xuejun Jiang ◽  
Qianghu Tang ◽  
Jun Zhang ◽  
Hong Wang ◽  
Lulu Bai ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Zinc Ions

Toxicokinetics of zinc-oxide nanoparticles and zinc ions in the earthworm Eisenia andrei

2017 ◽  
Vol 143 ◽  
pp. 151-158 ◽  
Author(s):  
Zuzanna M. Świątek ◽  
Cornelis A.M. van Gestel ◽  
Agnieszka J. Bednarska
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Zinc Ions ◽  
Eisenia Andrei

scholarly journals Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach

Nanoscale ◽  
2014 ◽  
Vol 6 (11) ◽  
pp. 6102-6114 ◽  
Author(s):  
Sarah Triboulet ◽  
Catherine Aude-Garcia ◽  
Lucie Armand ◽  
Adèle Gerdil ◽  
Hélène Diemer ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Cellular Responses ◽  
Oxide Nanoparticles ◽  
Zinc Ions ◽  
Proteomic Approach ◽  
Targeted Approach

A combined proteomics and targeted approach has been used to better understand the cellular responses to zinc oxide nanoparticles.

Absorption of zinc ions dissolved from zinc oxide nanoparticles in the tobacco callus improves plant productivity

2019 ◽  
Vol 138 (2) ◽  
pp. 377-385 ◽  
Author(s):  
Shizue Yoshihara ◽  
Kasumi Yamamoto ◽  
Yoshino Nakajima ◽  
Satomi Takeda ◽  
Kensuke Kurahashi ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Plant Productivity ◽  
Oxide Nanoparticles ◽  
Tobacco Callus ◽  
Zinc Ions

scholarly journals Cytotoxic and Membrane Cholesterol Effects of Ultraviolet Irradiation and Zinc Oxide Nanoparticles on Chinese Hamster Ovary Cells

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2979 ◽  
Author(s):  
Regina Kuebodeaux ◽  
Paul Bernazzani ◽  
Thi Nguyen
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Chinese Hamster Ovary ◽  
Mammalian Cells ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Carcinogenic Effects ◽  
Uv Visible ◽  
Uv C

Zinc Oxide (ZnO) nanoparticles are suspected to produce toxic effects toward mammalian cells; however, discrepancies in the extent of this effect have been reported between different cell lines. Simultaneously, high levels of ultraviolet (UV-C) radiation can have carcinogenic effects. The mechanism of this effect is also not well understood. Due to similarities in phenotype morphology after cell exposure to ZnO nanoparticles and UV-C irradiation, we emit the hypothesis that the toxicity of both these factors is related to damage of cellular membranes and affect their sterol content. Wild-type Chinese Hamster Ovary (CHO-K1) cells were exposed to ZnO nanoparticles or UV-C radiation. The amount of absorbed ZnO was determined by UV-visible spectroscopy and the changes in sterol profiles were evaluated by gas chromatography. Cell viability after both treatments was determined by microscopy. Comparing morphology results suggested similarities in toxicology events induced by ZnO nanoparticles and UV exposure. UV-C exposure for 360 min disrupts the sterol metabolic pathway by increasing the concentration of cholesterol by 21.6-fold. This increase in cholesterol production supports the hypothesis that UV irradiation has direct consequences in initiating sterol modifications in the cell membrane.

scholarly journals Zinc oxide nanoparticles effectively regulate autophagic cell death by activating autophagosome formation and interfering with their maturation

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Zixuan Liu ◽  
Xuying Lv ◽  
Lei Xu ◽  
Xuting Liu ◽  
Xiangyu Zhu ◽  
...  
Keyword(s):  
Cell Death ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Autophagic Cell Death ◽  
Oxide Nanoparticles ◽  
Zinc Ions ◽  
Zno Nps ◽  
Toxicological Effects ◽  
Major Mode ◽  
Free Zinc

Abstract Background With the development of zinc oxide nanoparticles (ZnO NPs) in the field of nanotechnology, their toxicological effects are attracting increasing attention, and the mechanisms for ZnO NPs neurotoxicity remain obscure. In an attempt to address concerns regarding neurotoxicity of ZnO NPs, we explored the relationship between free zinc ions, reactive oxygen species (ROS) and neurotoxic mechanisms in ZnO NPs-exposed PC12 cells. Result This study demonstrated the requirement of free zinc ions shed by ZnO NPs to over generation of intracellular ROS. Next, we identified autophagic cell death was the major mode of cell death induced by ZnO NPs, and autophagosome accumulation resulted from not only induction of autophagy, but also blockade of autophagy flux. We concluded that autophagic cell death, resulting from zinc ions-ROS-c-Jun N-terminal kinase (JNK)-autophagy positive feedback loop and blockade of autophagosomal-lysosomal fusion, played a major role in the neurotoxicity of ZnO NPs. Conclusion Our study contributes to a better understanding of the neurotoxicity of ZnO NPs and might be useful for designing and developing new biosafety nanoparticles in the future.

Sign in / Sign up

Export Citation Format

Share Document