Development of a Suite of Luciferase Gene Probes for the Screening and Detection of Marine Bioluminescent Systems and Organisms

Gene probes developed locally for both enteric Adenoviruses 40 and 41 were used to determine whether these viruses were present in both raw and treated waters. Approximately sixty water samples were concentrated by ultrafiltration and analysed directly for the presence of enteric adenoviruses. Three pretreatment techniques, namely sephadex columns, cellulose fibre and GenecleanTM were tested for the removal of inhibitory substances from concentrated water samples. The effect of chlorine treatment on viral detection using gene probe hybridization was also examined by exposing adenoviruses to chlorine concentrations of up to 20mg/l for 1 hour. Enteric adenoviruses were detected in up to 59% of both raw and treated waters analysed. Cellulose fibre and GenecleanTM were found to successfully remove inhibitory substances from concentrated raw waters. Viral DNA was detected after exposure to a range of chlorine concentrations indicating that the viruses detected in the treated waters may have been inactivated virus particles.

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Gene probes 2: a practical approach

General Pharmacology The Vascular System ◽

10.1016/s0306-3623(97)84209-7 ◽

1997 ◽

Vol 28 (3) ◽

pp. 481-482

Keyword(s):

Practical Approach ◽

Gene Probes

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Genomic Structure of the Luciferase Gene from the Bioluminescent Beetle,Nyctophilacf.Caucasica

Journal of Insect Science ◽

10.1673/031.006.3701 ◽

2006 ◽

Vol 6 (37) ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

John C. Day ◽

Mohammad J. Chaichi ◽

Iraj Najafil ◽

Andrew S. Whiteley

Keyword(s):

Genomic Structure ◽

Luciferase Gene

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Expression of Luciferase Gene Under Control of the puf Promoter from Rhodobacter sphaeroides

Twentieth Symposium on Biotechnology for Fuels and Chemicals ◽

10.1007/978-1-4612-1604-9_31 ◽

1999 ◽

pp. 337-345

Author(s):

Lyudmila Vasilyeva ◽

Masato Miyake ◽

Chikashi Nakamura ◽

Eiji Nakada ◽

Anatoly Tsygankov ◽

...

Keyword(s):

Rhodobacter Sphaeroides ◽

Luciferase Gene

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Applications of Gene Probes for the Detection of Foodborne Pathogens

Food Microbiology and Analytical Methods ◽

10.1201/9781482269833-10 ◽

1997 ◽

pp. 131-198

Keyword(s):

Foodborne Pathogens ◽

Gene Probes

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Efficient mobilization of E1-deleted adenovirus type 5 vectors by wild-type adenoviruses of other serotypes

Journal of General Virology ◽

10.1099/0022-1317-83-6-1311 ◽

2002 ◽

Vol 83 (6) ◽

pp. 1311-1314 ◽

Cited By ~ 11

Author(s):

Hendrik J. Rademaker ◽

Mohamed A. Abou El Hassan ◽

Gijs A. Versteeg ◽

Martijn J. W. E. Rabelink ◽

Rob C. Hoeben

Keyword(s):

Hepg2 Cells ◽

Adenovirus Type ◽

Firefly Luciferase ◽

Luciferase Gene ◽

Wild Type ◽

Genome Packaging ◽

Firefly Luciferase Gene ◽

Adenovirus Vectors ◽

Initiation Of Dna Replication ◽

Adenovirus Type 5

Mobilization of replication-deficient adenovirus vectors can lead to spread and shedding of the vector. Here we show that in cultured HepG2 cells wild-type (wt) adenoviruses of subgroup A (Ad12), B (Ad7, 11 and 16), C (Ad1, 2 and 5) and E (Ad4) can efficiently mobilize Ad5CMVluc, a ΔE1ΔE3-Ad5 vector carrying the firefly luciferase gene as reporter. In addition, we show that Ad5CMVluc can be propagated on Ad12E1-transformed human embryonic retinoblasts. This provides evidence that expression of the E1 region of Ad12 is sufficient for mobilizing ΔE1-Ad5-derived vectors. Thus, in therapeutic applications of replication-defective Ad vectors any active Ad infection is of potential concern, independent of the serotype involved. To prevent vector mobilization by wt Ads, new vectors should be developed in which essential functions such as the initiation of DNA replication and genome packaging are restricted.

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