Expression of Luciferase Gene Under Control of the puf Promoter from Rhodobacter sphaeroides

1999 ◽  
pp. 337-345
Author(s):  
Lyudmila Vasilyeva ◽  
Masato Miyake ◽  
Chikashi Nakamura ◽  
Eiji Nakada ◽  
Anatoly Tsygankov ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Luciferase Gene

Expression of Luciferase Gene Under Control of the puf Promoter from Rhodobacter sphaeroides

1999 ◽  
Vol 77 (1-3) ◽  
pp. 337-346 ◽  
Author(s):  
Lyudmila Vasilyeva ◽  
Masato Miyake ◽  
Chikashi Nakamura ◽  
Eiji Nakada ◽  
Anatoly Tsygankov ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Luciferase Gene

Nomenclature Abstract for Rhodobacter sphaeroides (van Niel 1944) Imhoff et al. 1984.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
Keyword(s):  
Rhodobacter Sphaeroides

Development of a Suite of Luciferase Gene Probes for the Screening and Detection of Marine Bioluminescent Systems and Organisms

2007 ◽  
Author(s):  
M. D. Iglesia-Rodriguez ◽  
Oscar M. Schofield ◽  
Scott M. Glenn ◽  
Mark Moline
Keyword(s):  
Luciferase Gene ◽  
Gene Probes

ВЛИЯНИЕ ЗАМЕНЫ ЛЕЙЦИНА M196 НА ГИСТИДИН НА ЭЛЕКТРОННУЮ СТРУКТУРУ ПЕРВИЧНОГО ДОНОРА ЭЛЕКТРОНА И ПЕРЕНОС ЭЛЕКТРОНА В РЕАКЦИОННЫХ ЦЕНТРАХ Rhodobacter sphaeroides

2019 ◽  
Vol 84 (5) ◽  
pp. 682-691
Author(s):  
А.А. Забелин ◽  
Т.Ю. Фуфина ◽  
А.М. Христин ◽  
Р.А. Хатыпов ◽  
В.А. Шкуропатова ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides

Metabolically versatile Rhodobacter sphaeroides as a robust biocatalyst for H2 production from lignocellulose-derived mix substrates

Fuel ◽  
2021 ◽  
Vol 302 ◽  
pp. 121108
Author(s):  
Jun Hu ◽  
Wenwen Wei ◽  
Qing Li ◽  
Wen Cao ◽  
Anlong Zhang ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
H2 Production

scholarly journals Multiple Chromosomes in Bacteria: The Yin and Yang of trp Gene Localization in Rhodobacter sphaeroides 2.4.1

Genetics ◽  
1999 ◽  
Vol 153 (2) ◽  
pp. 525-538
Author(s):  
Chris Mackenzie ◽  
Adrian E Simmons ◽  
Samuel Kaplan
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Biosynthetic Pathway ◽  
Southern Blotting ◽  
Physical Map ◽  
Functional Integration ◽  
Bacterial Genome ◽  
Single Copy ◽  
Gene Localization ◽  
Yin And Yang ◽  
Trp Genes

Abstract The existence of multiple chromosomes in bacteria has been known for some time. Yet the extent of functional solidarity between different chromosomes remains unknown. To examine this question, we have surveyed the well-described genes of the tryptophan biosynthetic pathway in the multichromosomal photosynthetic eubacterium Rhodobacter sphaeroides 2.4.1. The genome of this organism was mutagenized using Tn5, and strains that were auxotrophic for tryptophan (Trp-) were isolated. Pulsed-field gel mapping indicated that Tn5 insertions in both the large (3 Mb CI) and the small (0.9 Mb CII) chromosomes created a Trp- phenotype. Sequencing the DNA flanking the sites of the Tn5 insertions indicated that the genes trpE-yibQ-trpGDC were at a locus on CI, while genes trpF-aroR-trpB were at locus on CII. Unexpectedly, trpA was not found downstream of trpB. Instead, it was placed on the CI physical map at a locus 1.23 Mb away from trpE-yibQ-trpGDC. To relate the context of the R. sphaeroides trp genes to those of other bacteria, the DNA regions surrounding the trp genes on both chromosomes were sequenced. Of particular significance was the finding that rpsA1, which encodes ribosomal protein S1, and cmkA, which encodes cytidylate monophosphate kinase, were on CII. These genes are considered essential for translation and chromosome replication, respectively. Southern blotting suggested that the trp genes and rpsA1 exist in single copy within the genome. To date, this topological organization of the trp “operon” is unique within a bacterial genome. When taken with the finding that CII encodes essential housekeeping functions, the overall impression is one of close regulatory and functional integration between these chromosomes.

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