scholarly journals Adventitious Bud Formation from Callus Cultures of Japanese Persimmon

HortScience ◽  
1992 ◽  
Vol 27 (3) ◽  
pp. 259-261 ◽  
Author(s):  
Ryutaro Tao ◽  
Akira Sugiura
Keyword(s):  
High Frequency ◽  
Callus Cultures ◽  
Leaf Primordia ◽  
Adventitious Bud ◽  
Diospyros Kaki ◽  
Naphthaleneacetic Acid ◽  
Adventitious Buds ◽  
Japanese Persimmon ◽  
Adventitious Bud Formation ◽  
Young Leaves

Callus cultures were initiated in the dark from leaf primordia, stem internodes, and young leaves of adult Japanese persimmon (Diospyros kaki L.) to induce adventitious buds. A high frequency of regeneration occurred on Murashige and Skoog medium (MS) with half the normal NH4NO3 and KNO3 concentration (1/2N) and containing 10 μm zeatin or 1 μm 4PU-30 in combination with 0.1 μm IAA, or MS(1/2N) medium containing 0.03 to 0.1 μ m IAA or 0.01 to 0.03 μm NAA combined with 10 μm zeatin. No significant differences in the capacity of regeneration were observed among the calli from different explant sources. Only eight of 16 cultivars formed adventitious buds on MS(1/2N) medium containing 10 μm zeatin and 0.1 μm IAA, with the percentage of explants forming adventitious buds ranging from 2% to 72%. Chemical names used: indole3-acetic acid (IAA); 1-naphthaleneacetic acid (NAA); N-phenyl-N'-(2-chloro-4-pyridyl)urea (4PU-30).

scholarly journals Induction of Adventitious buds from stem explants in Aoectochilus formosanus

2019 ◽  
Vol 131 ◽  
pp. 01131
Author(s):  
Yingbin Xue ◽  
Fengyi Xiao ◽  
Xiaohao Li ◽  
Huamei Chen ◽  
Gangshun Rao ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Adventitious Bud ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Adventitious Buds ◽  
High Concentration ◽  
Diethyl Aminoethyl Hexanoate ◽  
Adventitious Bud Regeneration ◽  
Stem Segments

In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.

Abnormalities of Seed Development in a Parthenocarpic Japanese Persimmon, Diospyros kaki cv. Hiratanenashi

1990 ◽  
Vol 59 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Masashi ISHIDA ◽  
Masami KONISHI ◽  
Akira KITAJIMA ◽  
Yoshitsugu SOBAJIMA
Keyword(s):  
Seed Development ◽  
Diospyros Kaki ◽  
Japanese Persimmon

Utilization of Different Seedling Explants for in Vitro Propagation of Hibiscus syriacus

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 478e-479
Author(s):  
M.M. Jenderek ◽  
A.J. Olney
Keyword(s):  
Callus Formation ◽  
Adventitious Bud ◽  
Root Explants ◽  
Petiole Explants ◽  
Bud Formation ◽  
Hypocotyl Explants ◽  
Leaf Petiole ◽  
Adventitious Bud Formation ◽  
Hibiscus Syriacus

Hibiscus syriacus is a difficult species in micropropagation due to its endogenous contamination and recalcitrant shoot formation; therefore, studies on using explants other than shoot tip or axillary buds of growing shrubs were initiated. Three different seedling fragments (root, hypocotyl, and leaf petiole) from aseptically germinated seedlings of hibiscus (var. Aphrodite) were evaluated for adventitious bud formation, shoot and leaf development. The explants were cultured on McCown's woody plant basal salt medium supplemented with KNO3 (800 mg/L), adenine sulfate (80 mg/L) and MS vitamins containing BA or 2iP or TDZ at 0.5, 1.0, 2.2, 4.4 and 10 mM. Adventitious buds were present on all of the three different explants grown on medium containing TDZ; however, the most abundant bud formation, with many small leaves originating from callus was observed on hypocotyl explants cultured on medium with 1 mM of TDZ. Petiole explants were the most frequent to develop short shoots (≈15 mm) and one to nine leaves without callus formation, where 70% of hypocotyl and the root explants formed leaves originating from callus. Callus was induced on all explant types regardless of the level or type of cytokinin used. However, the number of shoots produced by any explant type was low, petioles cultured on 0.5 and 1mM of TDZ were the most suitable material for non-callus shoot development in H. syriacus. Hypocotyl explants proved to be an excellent source for adventitious bud formation but their ability to develop shoots needs to be investigated.

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