Micropropagation of Buttonwood Tree (Conocarpus erectus) through Axillary Shoot Proliferation

A method for micropropagation of Conocarpus erectus through axillary shoot proliferation is presented. Shoot tips were excised from adult donor tree and cultured for 4 weeks on Murashige and Skoog’s (MS) medium supplemented with 3 mg·L−1 gibberellic acid (GA3) to induce sprouting of shoots and formation of axillary shoots. Conocarpus erectus shoots were cultured for 6 weeks on MS medium supplemented with different concentrations and combinations of plant growth regulators (PGRs) and proliferation of the shoots was monitored. The type and concentration of cytokinins applied had a significant influence on shoot proliferation responses. Supplementation with 6-benzylaminopurine (BAP) increased the rate of shoot proliferation compared with other cytokinins. The use of BAP in combination with auxins such as indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) resulted in an increased number of shoots per explant compared with treatment with BAP alone. A combination of 2 mg·L−1 BAP and 0.5 mg·L−1 IBA produced the highest number of axillary shoots (7.8 shoots/explant). The best rooting medium was full-strength MS medium supplemented with 1 mg·L−1 IBA; this treatment yielded 80% rooting with an average of 3.5 roots per plantlet. All regenerated plantlets were successfully acclimatized to greenhouse conditions.

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Micropropagation and Genetic Fidelity of the Regenerants of Aglaonema ‘Valentine’ Using Randomly Amplified Polymorphic DNA

HortScience ◽

10.21273/hortsci.51.4.398 ◽

2016 ◽

Vol 51 (4) ◽

pp. 398-402 ◽

Cited By ~ 1

Author(s):

Mohammed Elsayed El-Mahrouk ◽

Yaser Hassan Dewir ◽

Yougasphree Naidoo

Keyword(s):

In Vitro Regeneration ◽

Shoot Proliferation ◽

Genetic Fidelity ◽

Naphthalene Acetic Acid ◽

Axillary Shoot ◽

Ms Medium ◽

Randomly Amplified Polymorphic Dna ◽

Simple Protocol ◽

Regenerated Plantlets

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

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In vitro propagation of lnula royleana DC

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2004.001 ◽

2011 ◽

Vol 73 (1) ◽

pp. 5-8 ◽

Cited By ~ 3

Author(s):

Anna Stojakowska ◽

Janusz Malarz

Keyword(s):

In Vitro Propagation ◽

Shoot Proliferation ◽

Cotyledonary Node ◽

Axillary Shoot ◽

Ms Medium ◽

Axillary Shoots ◽

Axillary Shoot Proliferation ◽

Primary Explants ◽

Cotyledonary Node Explants

A micropropagation method, through axillary shoot proliferation, was elaborated for <em>Inula royleana </em>DC. (Asteraceae), a medicinal plant native of Himalaya. Primary explants (cotyledonary node explants) and secondary explants (node explants of in vitro regenerated shoots) of the plant, inoculated on MS medium supplemented with 0.1 μM NAA and 5.0 μM kinetin, regenerated 3.4 ± 1.2 and 5.1 ± 1.9 axillary shoots per explant, respectively. The regenerated shoots were easily rooting and adapting to growth in soil.

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Micropropagation of Members of the Cactaceae Subtribe Cactinae

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.115.2.337 ◽

1990 ◽

Vol 115 (2) ◽

pp. 337-343 ◽

Cited By ~ 31

Author(s):

Philip W. Clayton ◽

John F. Hubstenberger ◽

Gregory C. Phillips ◽

S. Ann Butler-Nance

Keyword(s):

Shoot Proliferation ◽

Shoot Tip ◽

Axillary Shoot ◽

Axillary Shoots ◽

Axillary Shoot Proliferation ◽

Shoot Production ◽

Series Of Experiments ◽

Basal Media ◽

Free Media ◽

Shoot Tip Explants

Micropropagation of 11 rare or endangered cacti species belonging to the subtribe Cactinae was achieved by rooting of proliferated axillary shoots. Shoot tip explants were obtained from seedlings of Escobaria missouriensis D.R. Hunt, E. robbinsorum (Earle) D.R. Hunt, Sclerocactus spinosior (Engelm.) Woodruff & L. Benson, and Toumeya papyracantha (Engelm.) Br. & Rose, and from mature plants of Mammillaria wrightii Engelm., Pediocactus bradyi L. Benson, P. despainii Welsh & Goodrich, P. knowltonii L. Benson, P. paradinei B.W. Benson, P. winkleri Heil, and S. mesae-verdae (Boissevain) L. Benson. Three or four species were used in each of a series of experiments investigating the effects of basal media and auxin and cytokinin types and concentrations on axillary shoot proliferation. Low or no auxin but moderate to high cytokinin concentrations were required for axillary shoot production. All species rooted spontaneously on hormone-free media; however, several species rooted better on media containing auxin. All species were re-established in the greenhouse.

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Micropropagation of Clerodendrum phlomidis L.F.

Journal of Horticultural Research ◽

10.1515/johr-2016-0003 ◽

2016 ◽

Vol 24 (1) ◽

pp. 21-28 ◽

Cited By ~ 1

Author(s):

Mafatlal M. Kher ◽

Deepak Soner ◽

Neha Srivastava ◽

Murugan Nataraj ◽

Jaime A. Teixeira da Silva

Keyword(s):

Callus Formation ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

Nodal Explants ◽

Axillary Shoot ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Axillary Shoot Proliferation ◽

Clerodendrum Phlomidis

Abstract Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl) adenine (2iP), trans-zeatin (Zea) and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) in vitro rooted plantlets were successfully acclimatized under natural conditions.

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In Vitro Propagation of Eriostemon myoporoides and Eriostemon `Stardust'

HortScience ◽

10.21273/hortsci.29.6.686 ◽

1994 ◽

Vol 29 (6) ◽

pp. 686-688 ◽

Cited By ~ 5

Author(s):

James R. Ault

Keyword(s):

Root Length ◽

Basal Medium ◽

Shoot Proliferation ◽

Shoot Length ◽

Axillary Shoot ◽

Naphthaleneacetic Acid ◽

Stem Explants ◽

Ms Medium ◽

Axillary Shoot Proliferation

Optimal axillary shoot proliferation was obtained from stem explants of a clone of Eriostemon myoporoides DC. on Murashige and Skoog (MS) basal medium containing 0.1 mg BA/liter, and of Eriostemon `Stardust' on MS medium containing 0.5 mg BA/liter. Overall average number of shoots and shoot lengths for all treatments was greater for E. `Stardust' (22.4 shoots and 12.1-mm shoot length) than for E. myoporoides (4.5 shoots and 8.3-mm shoot length). Maximum percent rooting of E. myoporoides (42%) and E. `Stardust' (95%) was obtained on MS medium supplemented with 1.0 mg K-IBA/liter for E. myoporoides and 0.1 mg NAA/liter for E. `Stardust'. Overall average percent rooting and root lengths were greater for E. `Stardust' (42% rooting and 11.0-mm root length) than for E. myoporoides (27% rooting and 2.3-mm root length). For E. `Stardust', reducing sucrose in the rooting medium from 50 to 25 g·liter-1 significantly decreased overall average percent rooting to 1670 and root length to 6.8 mm. Plantlets of both clones were acclimatized in the greenhouse and transferred successfully to soil, although survival was <7070. Chemical names used: N -(phenylmethyl) -l H -purine-6-amine (BA); potassium-l H -indole-3-butyric acid (K-IBA); l-naphthaleneacetic acid (NAA).

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COMPARISON OF TWO METHODS FOR IN VITRO PROPAGATION OF RAUWOLFIA SERPENTINA FROM NODAL EXPLANTS

10.53879/id.44.07.p0514 ◽

2007 ◽

Vol 44 (07) ◽

pp. 514-519 ◽

Cited By ~ 1

Author(s):

Ved Prakash Pandey ◽

Jose Kudakasseril ◽

Elizabeth Cherian ◽

George Patani ◽

Keyword(s):

Acetic Acid ◽

In Vitro Propagation ◽

Shoot Proliferation ◽

Nodal Explants ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

In Vitro Multiplication ◽

Rauwolfia Serpentina ◽

In Vitro Micropropagation

Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.

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Micropropagation of Adult Red Maple and Sugar Maple

HortScience ◽

10.21273/hortsci.33.3.477e ◽

1998 ◽

Vol 33 (3) ◽

pp. 477e-478 ◽

Cited By ~ 1

Author(s):

David A. Connolly ◽

John E. Preece ◽

J.W. Van Sambeek

Keyword(s):

Sugar Maple ◽

Woody Plant ◽

Acer Rubrum ◽

Shoot Proliferation ◽

Axillary Shoot ◽

Red Maple ◽

Axillary Shoots ◽

Axillary Shoot Proliferation ◽

Woody Plant Medium ◽

The Media

Micropropagation studies were conducted to determine the best methods to achieve axillary shoot proliferation for adult Acer rubrum (red maple) and A. saccharium (sugar maple). The first experiment was conducted to compare the effects of 0.001, 0.01, 0.1 μM, 1 μM, and 10 μM thidiazuron (TDZ) using Driver-Kuniyuki-Walnut medium (DKW). The second experiment was conducted to examine the effects of DKW, Woody Plant Medium (WPM) and Long and Preece (LP) media in factorial combination with 0.01 and 0.1 μM TDZ. The third experiment was conducted to study the transfer timing (14 or 28 days) and the media solidification (agar-solidified or stationary liquid) on sugar maple. Both red maple and sugar maple explants on DKW with 0.1 μM TDZ produced the most and longest axillary shoots; however, sugar maple produced fewer axillary shoots than red maple. Red maple explants produced the most callus on DKW with 10 μM TDZ and the least on DKW with 0.001 μM TDZ. Sugar maple explants produced more shoots when explants were placed horizontally and transferred every 14 days than when placed vertically or transferred less frequently.

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Shoot Proliferation, Callus Induction And Plant Regeneration In Tripsacum Laxum Nash

10.21203/rs.3.rs-880060/v1 ◽

2021 ◽

Author(s):

Yuping Xiong ◽

Jinhui Pang ◽

Kunlin Wu ◽

Jaime A. Teixeira Silva ◽

Xinhua Zhang ◽

...

Keyword(s):

Peat Soil ◽

Shoot Proliferation ◽

Multiple Shoots ◽

Dichlorophenoxyacetic Acid ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Axillary Shoots ◽

Rooting Medium ◽

2,4 Dichlorophenoxyacetic Acid

Abstract The peduncles of Tripsacum laxum Nash were used as explants to induce axillary shoots. Multiple shoots were proliferated on Murashige and Skoog (MS) medium to establish, for the first time, efficient shoot proliferation and plant in vitro regeneration systems. Optimal shoot proliferation medium was MS with 3.0 mg/L 6-benzyladenine (BA) and 0.2 mg/L α-naphthaleneacetic acid (NAA), resulting in a shoot proliferation coefficient of 11.0 within 45 d. Optimal rooting medium was MS with 0.1 mg/L NAA and/or 0.1 mg/L indole-3-butyric acid (IBA), inducing 100% root formation from shoots within 30 d. When young roots, leaf sheaths and shoot bases were used as explants, MS medium with 1.0 mg/L thidiazuron (TDZ) and 0.2 mg/L BA induced most shoots, with the least callus. Shoot bases induced beige-white callus and shoots directly on MS medium with 1.0 mg/L TDZ and 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), while leaf sheaths induced beige-white callus and shoots directly on MS medium with 1.0 mg/L TDZ and 0.2 mg/L BA. Rooted plantlets showed 99.3% survival when transplanted into a substrate of vermiculite: peat soil (1:3, v/v).

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High-efficiency Propagation of Mature ‘Washington Navel’ Orange and Juvenile ‘Carrizo’ Citrange Using Axillary Shoot Proliferation

HortTechnology ◽

10.21273/horttech.26.3.278 ◽

2016 ◽

Vol 26 (3) ◽

pp. 278-286 ◽

Cited By ~ 1

Author(s):

Maria Luiza De Oliveira ◽

James G. Thomson ◽

Ed Stover

Keyword(s):

Root Length ◽

Shoot Proliferation ◽

Axillary Shoot ◽

Navel Orange ◽

Axillary Shoots ◽

Axillary Shoot Proliferation ◽

Full Strength ◽

Carrizo Citrange ◽

Washington Navel

In vitro axillary shoot proliferation can be used to increase availability of citrus (Citrus) types in high demand, while limiting somaclonal variation. However, established protocols could be improved to increase efficiency. Therefore, this study investigated some factors [plant growth regulators (PGRs), basal media, and successive subculturing] which affect the in vitro axillary shoot proliferation of mature ‘Washington Navel’ orange (Citrus sinensis) and juvenile ‘Carrizo’ citrange (C. sinensis × Poncirus trifoliata). In ‘Washington Navel’ orange, maximum axillary shoot induction (66.9% explants producing axillary shoots with a mean of 2.45 shoots per explant) was obtained in Driver and Kuniyuki walnut (DKW) medium supplemented with 0.1 mg·L−1 6-benzylaminopurine (BA), 0.05 mg·L−1 naphthalene acetic acid (NAA) along with 1 mg·L−1 6-furfurylaminopurine [kinetin (kin)], whereas in ‘Carrizo’ citrange, axillary shoot production was greatest (82.6% and 87.5% of explants producing axillary shoots with a mean of 4.3 and 4.1 shoots per explant) at 1.0 or 2.0 mg·L−1 BA in DKW medium. The initial nodal propagules (with basal tissue remaining from removed shoots) were repeatedly subcultured for six times every 4 weeks onto DKW medium with the same levels of PGRs used for initial culturing. Woody plant medium (WPM), Murashige and Skoog medium (MS), and DKW were also compared for rooting at quarter to full strength for salt components, all amended with 2.0 mg·L−1 indolebutyric acid (IBA) and 0.5 mg·L−1 NAA. MS at full strength provided the highest rooting in ‘Carrizo’ citrange (93%) and longest root length (58 mm), whereas half-strength MS provided the highest rooting in ‘Washington Navel’ orange (60% to 61%) and the longest roots (26 mm). Addition of 1 μm spermidine to the rooting medium enhanced root length only for ‘Washington Navel’ orange on full-strength MS, but accelerated rooting for both cultivars on all media. The plantlets were successfully transferred to greenhouse conditions, exhibiting normal development, with high uniformity, and no evidence of somaclonal variation.

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Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽

10.21273/hortsci.40.6.1832 ◽

2005 ◽

Vol 40 (6) ◽

pp. 1832-1837 ◽

Cited By ~ 1

Author(s):

Donita L. Bryan ◽

Michael A. Arnold ◽

R. Daniel Lineberger ◽

W. Todd Watson

Keyword(s):

Tissue Culture ◽

Acetic Acid ◽

Butyric Acid ◽

Shoot Proliferation ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Root Number ◽

Indole Butyric Acid ◽

Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

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