scholarly journals Genetic Diversity among Witchhazel Cultivars Based on Randomly Amplified Polymorphic DNA Markers

1997 ◽  
Vol 122 (4) ◽  
pp. 529-535 ◽  
Author(s):  
Robert D. Marquard ◽  
Eric P. Davis ◽  
Emily L. Stowe

Forty selections, including 37 cultivars of Hamamelis spp., were evaluated for genetic similarities using randomly amplified polymorphic DNA (RAPD) markers. Cluster analysis identified seven groups, which included three groups of H. ×intermedia cultivars, two groups of H. vernalis, and one group each of H. mollis and H. japonica. Three H. ×intermedia cultivars, `Arnold Promise', `Westerstede', and `Carmine Red', did not group closely with the other 20 cultivars of H. ×intermedia. Selections of the North American species H. vernalis were quite distinct from the Asiatic selections. However, data are presented that suggest hybridization exist between Asiatic Hamamelis spp. and H. vernalis. Genetic similarities between known half-sib families provides evidence that the cultivar pairs `Arnold Promise'—`Winter Beauty' and `Carmine Red'—`Hiltingbury' are, themselves, not likely half-sibs.

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 571-580
Author(s):  
Vladan Popovic ◽  
Aleksandar Lucic ◽  
Danijela Ristic ◽  
Ljubinko Rakonjac ◽  
Sabahudin Hadrovic ◽  
...  

The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of detected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.


HortScience ◽  
1997 ◽  
Vol 32 (1) ◽  
pp. 122-124 ◽  
Author(s):  
Jing-Tian Ling ◽  
Roger Sauve ◽  
Nick Gawel

Randomly amplified polymorphic DNA (RAPD) techniques were used to compare the DNA from leaf tissues of nine commercial poinsettia (Euphorbia pulcherrima Wild ex Klotzsch) cultivars. Amplification occurred in 57 out of 60 (95%) tested primers. Nine primers that revealed polymorphisms among cultivars were selected for further evaluation. Forty-eight RAPD bands were scored from these primers, and 33 (69%) were polymorphic. All tested cultivars could be discriminated with seven bands generated from primers OPB7 and OPC13. Results of a UPGMA cluster analysis and principal components analysis placed the nine cultivars into two groups: one group consisted of `Jingle Bells', `Supjibi', and `V-17 Angelika', the other of `V-14 Glory', `Red Sails', `Jolly Red', and `Freedom'. `Lilo Red' and `Pink Peppermint' belonged to the latter group, but were relatively distant from other cultivars in that group. These results indicate that RAPDs are efficient for identification of poinsettia cultivars and for determination of the genetic relationships among cultivars.


1990 ◽  
Vol 122 (1) ◽  
pp. 131-171 ◽  
Author(s):  
Yves Bousquet

AbstractThe Nearctic species of the subgenus Anomophagus Reitter of the genus Rhizophagus Herbst are revised. Four taxa are recognized: R. brunneus brunneus Horn 1879, R. brunneus fenyesi Méquignon 1913a, R. pseudobrunneus sp.nov., and R. galbus sp.nov.For the other Nearctic species of the genus, a diagnosis, a type material section, and distributional notes are provided. Two new taxa are described, R. minutus rotundicollis and R. pusillus. Seven specific names are treated as new junior synonyms, namely (with the valid name in parentheses): R. longiceps Casey 1916 and R. rectus Casey 1916 (= R. cylindricus LeConte 1866); R. minutus quadriguttatus Méquignon 1913b (= R. minutus minutus Mmnerheim 1853); R. remotus luteus Méquignon 1913b (= R. remotus LeConte 1866); R. sculpturatus horni Méquignon 1913b (= R. sculpturatus Mannerheim 1852); R. dimidiatus testaceus Méquignon 1913b and R. dimidiatus assimilis Méquignon 1913b (= R. dimidiatus Mannerheim 1843). The subgenera name Syringobidia Casey 1916 (type species: R. cylindricus LeConte 1866) is treated as a new junior subjective synonym of Rhizophagus s.str. A key to all Nearctic species of Rhizophagus is provided with distribution maps of the species.


1965 ◽  
Vol 97 (5) ◽  
pp. 533-541 ◽  
Author(s):  
G. R. Hopping

AbstractGroup VI of North American Ips contains I. perturbatus (Eichhoff), I. hunteri Swaine, I. utahensis Wood and I. woodi Thatcher. A key and descriptions of species are given. Members of this group have the third declivital spine capitate with the tip conical and acute. The front of the head is evenly convex except in females of I. utahensis which have the lower part of the frons faintly elevated. I. woodi breeds in pine while the other three species breed in spruce.


Biologia ◽  
2008 ◽  
Vol 63 (1) ◽  
Author(s):  
Andrej Kormuťák ◽  
Seok-Woo Lee ◽  
Kyung-Nak Hong ◽  
Byeung-Hook Yang ◽  
Yong-Pyo Hong

AbstractArtificial crossing experiments involving 3 Abies species native to Korean peninsula and 5 other representatives of Abies revealed a high hybridological affinity between Abies koreana and A. nephrolepis. Both these species are reproductively isolated from A. holophylla. All the three Korean species were found to exhibit incompatible relationships with the North American species A. concolor. The species A. holophylla and A. koreana differ also in their abilities to intercross with the Mediterranean firs. The former has been successfully crossed with A. nordmanniana, A. alba and A. cilicica exhibiting 19.1–55.3% crossability, whereas the latter produced filled seeds only with A. nordmanniana reaching 46.4% crossability. A considerable differentiation is postulated to exist between the pair of species A. koreana and A. nephrolepis on the one side and A. holophylla on the other side.


1968 ◽  
Vol 100 (11) ◽  
pp. 1121-1137 ◽  
Author(s):  
Leonard A. Kelton

AbstractNine new species of Slaterocoris Wagner are described from North America: pilosus from British Columbia; alpinus from Colorado; apache from Arizona, Colorado, and Utah; flavipes, solidaginis, and sparsus from California; argenteus, grandis, and simplex from Durango, Mexico. The other species in the genus are: ambrosiae (Kngt.), atratus (Uhl.), atritibialis (Kngt.), breviatus (Kngt.), croceipes (Uhl.), hirtus (Kngt.), longipennis Kngt., mohri (Kngt.), pallidicornis (Kngt.), pallipes (Kngt.). robustus (Uhl.), rubrofemoratus Kngt., sheridani Kngt., stygicus (Say), and utahensis Kngt. Strongylocoris uniformis Van D. is placed in synonymy with Stiphrosoma robusta Uhl. Strongylocoris albibasis Knight does not belong to Slaterocoris and will be dealt with in a subsequent paper. All species are keyed and the male genitalia illustrated.


Author(s):  
Beibei Li ◽  
Xiucai Fan ◽  
Ying Zhang ◽  
Chonghuai Liu ◽  
Jianfu Jiang

Chinese wild Vitis is a useful gene source for resistance to biotic and abiotic stresses, although there is little research on its genetic diversity and structure. In this study, nine simple sequence repeat (SSR) markers were used to assess the genetic diversity and genetic structure among 100 Vitis materials. These materials included 77 indigenous accessions representing 23 of 38 wild Vitis species/cultivars in China, 18 V. vinifera cultivars, and the five North American species V. aestivalis, V. girdiana, V. monticola, V. acerifolia, and V. riparia. The SSR loci used in this study for establishing an international database (Vitis International Variety Catalogue) revealed a total of 186 alleles in 100 Vitis accessions. The mean values for the gene diversity (GD) and polymorphism information content (PIC) per locus were 0.91 and 0.90, respectively, which indicates that the discriminatory power of the markers is high. Based on the genetic distance data, the 100 Vitis accessions were divided into five primary clusters by cluster analysis, and five populations by structure analysis; these results indicate these Chinese wild grapes were more genetically close to European grapes than to North American species. In addition, the clustering patterns of most accessions correlated with the geographic distribution. An analysis of molecular variance (AMOVA) revealed that 3.28%, 3.27%, and 93.46% of the variance occurred between populations, between individuals within populations, and between individuals within the entire population, respectively. In addition, we identified three previously undescribed accessions (Wuzhi-1, MZL-5, and MZL-6) by cluster analysis. Our results reveal a high level of genetic diversity and variability in Vitis from China, which will be helpful in the use of genetic resources in future breeding programs. In addition, our study demonstrates that SSR markers are highly suitable for further genetic diversity analyses of Chinese wild grapes.


1965 ◽  
Vol 97 (2) ◽  
pp. 193-198 ◽  
Author(s):  
G. R. Hopping

AbstractGroup VII of North American Ips contains I. thomasi, new species, I. borealis Swaine and I. swainei R. Hopping. They are less than 4.0 mm. long and females have the front of the head or at least the vertex smooth and shining, impunctate, or with very fine sparse punctures; males are more coarsely granulate-punctate on the frons. The species are described and a key is given. All breed in Picea in Canada and northern United States.


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