scholarly journals Genetic Relationships among 10 Prunus Rootstock Species from China, Based on Simple Sequence Repeat Markers

2016 ◽  
Vol 141 (5) ◽  
pp. 520-526 ◽  
Author(s):  
Qijing Zhang ◽  
Dajun Gu
Keyword(s):  
Phylogenetic Analysis ◽  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Sequence Repeat ◽  
Breeding Programs ◽  
Banding Patterns ◽  
Simple Sequence Repeat Markers ◽  
Ssr Primers ◽  
Relationship Of ◽  
Simple Sequence

To improve the efficiency of breeding programs for Prunus rootstock hybrids in China, we analyzed the subgenus status and relationship of 10 Chinese rootstock species, by using 24 sets of simple sequence repeat (SSR) primers. The SSR banding patterns and phylogenetic analysis indicated that subgenus Cerasus is more closely related to subgenus Prunophora than to subgenus Amygdalus, and that subgenus Lithocerasus is more closely related to subgenus Prunophora and subgenus Amygdalus than to subgenus Cerasus. In addition, Prunus triloba was more closely related to Prunus tomentosa than to the members of subgenus Amygdalus. Therefore, we suggest that P. tomentosa and P. triloba should be assigned to the same group, either to subgenus Lithocerasus or Prunophora, and we also propose potential parent combinations for future Prunus rootstock breeding.

scholarly journals Genetic variation in Hydrangea macrophylla (Thunb.) Ser. in Russia based on simple sequence repeat markers

2018 ◽  
Vol 47 (4) ◽  
pp. 937-943
Author(s):  
Natalia Sukhikh ◽  
Valentina Malyarovskaya ◽  
Anastasiya Kamionskaya ◽  
Lidia Samarina ◽  
Svetlana Vinogradova
Keyword(s):  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Effective Number ◽  
Sequence Repeat ◽  
Base Pairs ◽  
Breeding Programs ◽  
Simple Sequence Repeat Markers ◽  
Hydrangea Macrophylla ◽  
High Level ◽  
Simple Sequence

Genetic diversity and genetic relationships among 39 accessions of Hydrangea macrophylla (Thunb.) Ser. were analyzed using 38 previously developed simple sequence repeat markers (SSRs). A total of 38 polymorphic primers representing 166 bands with an average of 4.53 polymorphic bands per primer were selected. The number of alleles detected per locus ranged from two to eight with a total of 163 alleles amplified. The size of the amplified fragments ranged from 70 to180 base pairs. The effective multiallelic markers with high level of heterozygosity (more than 0.7) and effective number of alleles (more than 3.5) were identified. In this study nine SSR markers showed clear polymorphisms. The dendrogram grouped all hybrids in three major clusters, and two of these clusters included only mophead cultivars. The lacecap cultivars clustered more closely to each other. The results of this research could be used in breeding programs of H. macrophylla.

Genetic relationships of boxwood (Buxus L.) accessions based on genic simple sequence repeat markers

2016 ◽  
Vol 64 (6) ◽  
pp. 1281-1293 ◽  
Author(s):  
Chandra S. Thammina ◽  
Richard T. Olsen ◽  
Matthew Kramer ◽  
Margaret R. Pooler
Keyword(s):  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Simple Sequence

scholarly journals Development of Simple Sequence Repeat Markers for Chionanthus retusus (Oleaceae) and Effective Discrimination of Closely Related Taxa

HortScience ◽  
2011 ◽  
Vol 46 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Renée S. Arias ◽  
Natascha Techen ◽  
Timothy A. Rinehart ◽  
Richard T. Olsen ◽  
Joseph H. Kirkbride ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Information ◽  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Breeding Programs ◽  
Simple Sequence Repeat Markers ◽  
Tropical Areas ◽  
Garden Plants ◽  
Trees And Shrubs ◽  
Simple Sequence

The genus Chionanthus (Oleaceae Hoffmans. & Link) includes deciduous or evergreen trees and shrubs distributed widely in tropical and sub-tropical areas, including a few temperate species. Although Chionanthus species are planted as ornamental garden plants and commercialized for natural products, genetic information for Chionanthus spp. is lacking. We created microsatellite-enriched libraries of Chionanthus retusus Lindl. & Paxton, assembled 1072 contigs, and detected 1010 repeats. The frequency of the repeats decreased with the increase in repeat length, and the most abundant motifs were: AG, AC, AAG, ACC, AT, and ACTC. We screened 384 markers on 12 accessions of four related taxa that included C. retusus, Chionanthus virginicus L., Chionanthus pygmaeus Small, and Osmanthus americanus (L.) Benth. & Hook. A total of 195 simple sequence repeat (SSR) markers amplified and discriminated six accessions of C. retusus and 57 SSR markers amplified and discriminated across the four Oleaceae species screened. To identify the best markers to use in future experiments, the “Unique Pattern Informative Combination” (UPIC) values were calculated for all the markers and the 100 markers that were most effective are reported here. The percentage of heterozygous loci across the 384 markers was lowest for C. retusus (29.3%) and highest for O. americanus (68.9%). The SSR markers developed here could assist in taxonomy and hybridization investigations for breeding programs and authentication of varieties used as medicinal plants.

scholarly journals Assessing the genetic relationships of Curcuma alismatifolia varieties using simple sequence repeat markers

2014 ◽  
Vol 13 (3) ◽  
pp. 7339-7346
Author(s):  
S. Taheri ◽  
T.L. Abdullah ◽  
N.A.P. Abdullah ◽  
Z. Ahmad ◽  
E. Karimi ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Genetic Relationships ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Simple Sequence
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