scholarly journals PCR Markers for Triticum speltoides Leaf Rust Resistance Gene Lr51 and Their Use to Develop Isogenic Hard Red Spring Wheat Lines

Crop Science ◽  
2005 ◽  
Vol 45 (2) ◽  
pp. 728-734 ◽  
Author(s):  
M. Helguera ◽  
L. Vanzetti ◽  
M. Soria ◽  
I. A. Khan ◽  
J. Kolmer ◽  
...  
Euphytica ◽  
2017 ◽  
Vol 213 (12) ◽  
Author(s):  
Neeraj Kumar Vasistha ◽  
Arun Balasubramaniam ◽  
Vinod Kumar Mishra ◽  
Jayasudha Srinivasa ◽  
Ramesh Chand ◽  
...  

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.


2017 ◽  
Vol 67 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Ali Aliakbari Sadeghabad ◽  
Ali Dadkhodaie ◽  
Bahram Heidari ◽  
Hooman Razi ◽  
Reza Mostowfizadeh-Ghalamfarsa

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