The influence of five crop rotations and the rotation phases (i.e., rotation-yr) on some soil organic matter characteristics was investigated in a long-term (23 yr) study carried out on an Orthic Dark Brown Chernozemic soil at Scott, Saskatchewan. The cropping systems included different cropping frequencies and crop types (cereals, oilseeds, and legume-hay). Soil samples were taken from the 0- to 7.5- and 7.5- to 15-cm depths in mid-September 1988, 2 wk after harvest of the grain crops (i.e., 2 mo after hay harvest and plowdown). Most effects of rotations, and rotation phases, on soil biological characteristics assessed, were significant primarily in the top 7.5-cm soil depth. Increasing the cropping frequency did not increase soil organic matter. Excessive preseeding tillage of stubble plots may have masked any potential advantage provided by frequent cropping. Including alfalfa (Medicago sativa L.) hay crops in rotation with grain crops decreased soil organic matter in the fallow and grain crop rotation phases of rotations. This was likely due to increased moisture stress depressing associated cereal production in this semiarid environment. As expected, rotation phase did not influence soil organic C, but alfalfa under-seeded into barley (Hordeum vulgare L.) increased soil organic nitrogen. We believe this was due to crop residue inputs from the seedling alfalfa. Microbial biomass C and N, C mineralization, the specific respiratory activity (ratio of CO2-C respired/microbial biomass C) and hydrolyzable amino acids were also greater in the rotation phases in which barley was underseeded with alfalfa. Carbon mineralization and specific respiratory activity were directly related to estimated crop residue-C returned to soil, but not residue-N. However, both were increased by including alfalfa in the rotation. Carbon mineralization and specific respiratory activity were more sensitive indexes of soil organic matter quality than biomass C and N per se. Hydrolyzable amino acids and amino sugars responded to the treatments in a manner similar to total soil organic N. Relative molar distribution of amino acids was unaffected by crop rotation or rotation phase. Potentially mineralizable N in this soil was low compared to other Canadian prairie soils, even though the total soil organic N of the Scott soil was relatively high. We concluded that (i) all soil biochemical characteristics studied are useful for assessing soil quality changes; (ii) when studying soil changes, thin (0- to 7.5-cm) soil slices are more likely to reveal treatment effects than thicker slices; (iii) all rotation phases should be analyzed whenever forage legumes are constituents of crop rotations. Key words: C mineralization; microbial biomass, amino acids, N mineralization, specific respiratory activity
Quantifying Charcoal Degradation and Negative Priming of Soil Organic Matter with a 14C-Dead Tracer