scholarly journals Optimized Vector for Conditional Gene Targeting in Mouse Embryonic Stem Cells

BioTechniques ◽  
2003 ◽  
Vol 34 (6) ◽  
pp. 1136-1140 ◽  
Author(s):  
Marcus Conrad ◽  
Markus Brielmeier ◽  
Wolfgang Wurst ◽  
Georg W. Bornkamm
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Conditional Gene Targeting

scholarly journals Targeting frequency for deletion vectors in embryonic stem cells.

1994 ◽  
Vol 14 (4) ◽  
pp. 2404-2410 ◽  
Author(s):  
H Zhang ◽  
P Hasty ◽  
A Bradley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Hypoxanthine Phosphoribosyltransferase ◽  
Genomic Deletions ◽  
Wide Range

We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7-kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.

scholarly journals Bi-allelic gene targeting in mouse embryonic stem cells

Methods ◽  
2011 ◽  
Vol 53 (4) ◽  
pp. 331-338 ◽  
Author(s):  
Peri H. Tate ◽  
William C. Skarnes
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Allelic Gene

scholarly journals Modulation of gene activity by consecutive gene targeting of one creatine kinase M allele in mouse embryonic stem cells

1991 ◽  
Vol 19 (10) ◽  
pp. 2637-2643 ◽  
Author(s):  
J. van Deursen ◽  
R. Lovell-Badge ◽  
F. Oerlemans ◽  
J. Schepens ◽  
B. Wieringa
Keyword(s):  
Stem Cells ◽  
Creatine Kinase ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Gene Activity

scholarly journals Pooling and PCR as a method to combat low frequency gene targeting in mouse embryonic stem cells

2006 ◽  
Vol 51 (2) ◽  
pp. 81-88 ◽  
Author(s):  
A. C. Brown ◽  
C. P. Lerner ◽  
J. H. Graber ◽  
D. J. Shaffer ◽  
D. C. Roopenian
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Low Frequency ◽  
Mouse Embryonic Stem Cells
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