<p>In vitro culture<br />techniques have become alternative to help overcome the<br />problems those are often encountered in the provision of<br />seeds through conventional means. Micropropagation<br />through apex culture in sugarcane has several advantages,<br />such as the produced plants have higher genetic stability,<br />high multiplication rate, and more healthy seeds (especially<br />virus-free)., The aims of the the research were to produce<br />seeds of two varieties of sugarcane, namely PS864 and<br />PS881, through apex culture. Laboratory-scale research was<br />conducted at the Indonesian Center for Agricultural<br />Biotechnology and Genetic Resources Research and<br />Development (ICABIOGRAD), Bogor, while sowing seeds<br />nursery was done in the Experimental Station of Gowa,<br />South Sulawesi Assessment Institute for Agricultural<br />Technology. The experiments consisted of initiation and<br />regeneration of apexes, shoots multiplication, rooting<br />induction, and acclimatization of plantlets. Research results<br />showed the initiation and regeneration of PS864 and PS881<br />through apex culture could be done on MS basic medium<br />containing 0.5 mg/l BAP. Shoot proliferation of both varieties<br />increased in the second subculture. Addition of 1 mg/l BAP<br />into medium in the second subculture resulted in higher<br />average number of shoots than that of 5 mg/l BAP, both for<br />PS864 and PS881. Addition of 1 mg/l and 5 mg/l kinetin<br />showed no significant differences for shoot numbers<br />compared to that of PS864 in medium containing 1 mg/l<br />BAP. The average number of PS881 shoots in multiplication<br />media containing 5 mg/l kinetin was higher than that of 1<br />mg/l kinetin. Increased concentrations of NAA and IBA from<br />0.1 mg/l to 0.5 mg/l in the MS medium were correlated to the<br />increased number of roots in PS864 shoots. Meanwhile, only<br />increased concentration of NAA that affected rooting percentage<br />of PS881. Acclimatization showed the percentage of<br />the plantlets grown in polybags was higher than that directly<br />grown in planting bed. The primary seeds (G0) produced in<br />these experiments were ready to be reproduced again to<br />obtain further stages.</p>
APPROBATION OF ISSR DNA-MARKERS FOR GENOTYPING OF GALÁNTHUS WORONOWII LOSINSK.. AND ANALYSIS OF GENETIC STABILITY OF PLANTS, OBTAINED BY IN VITRO CULTURE
