Leaf Color of Plants Regenerated through In Vitro Culture from Variegated Leaf Segments of Caladium.

1993 ◽  
Vol 62 (3) ◽  
pp. 619-624 ◽  
Author(s):  
Yu Zhu ◽  
Tetsuyuki Takemoto ◽  
Susumu Yazawa
Keyword(s):  
In Vitro Culture ◽  
Leaf Color ◽  
Leaf Segments ◽  
Variegated Leaf

scholarly journals Improvement of sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma irradiation and large scale plantlet production from M1 generation through in vitro culture

2016 ◽  
Vol 5 (1) ◽  
pp. 1-9
Author(s):  
Shamima Akter ◽  
N Alam ◽  
PK Roy
Keyword(s):  
Gamma Radiation ◽  
In Vitro Culture ◽  
Large Scale ◽  
Saccharum Officinarum ◽  
Plantlet Production ◽  
In Vitro Technique ◽  
Half Strength ◽  
Leaf Segments ◽  
Agronomical Traits

The present study was aimed to develop mutant sugarcane (Saccharum officinarum L. var. Isd. 39) using gamma radiation followed by regeneration of those mutants through in vitro technique. Five different doses of gamma radiation (10, 20, 30, 40 and 50 Gy) were applied to the sugarcane setts and among these doses 10 Gy irradiated mutant sugarcane showed the best agronomical traits. In addition, 10 Gy irradiated mutant sugarcane also contained higher sugar concentrations (14.25%) compared to control (14.23%). On the other hand, shoot tip and young folded leaf segments from the 10 Gy irradiated plants (M1 generation) were used as explants in in vitro culture. It was evident from the experiment that direct shoot regeneration was better than indirect regeneration. Young leaf segments produced the highest percentage (84%) of shoots when cultured on MS+1.0 mg/l Kin + 0.2 mg/l NAA. The mean number of shoots was recorded as 35 ± 1.12 in the same medium. Moreover, addition of 4% sucrose and 10% coconut water (CW) in the same medium increased the number of shoot up to 45 per culture. Best rooting was obtained from shoots cultured on half-strength of MS fortified with 1.0mg/l NAA and 0.5mg/l IBA. Well-rooted plantlets were transferred to poly bag containing soil and compost (2:1) for hardening. During hardening 70% plantlets survived, which were subsequently transferred to the experimental field.Jahangirnagar University J. Biol. Sci. 5(1): 1-9, 2016 (June)

scholarly journals Evaluation of protocorm formation in meristematic regions of roots and leaves of Miltonia spectabilis moreliana (Orchidaceae).

2015 ◽  
Vol 5 (1) ◽  
pp. 508-513
Author(s):  
Cristiano Pedroso-de-Moraes ◽  
Thiago de Souza-Leal ◽  
Rodrigo Pires Corrêa ◽  
Renaze Raiane Leme Paioli ◽  
Marco Aurélio Marteline
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Fresh Weight ◽  
Leaf Segments ◽  
Protocorm Formation

The aim of this study was to evaluate the effect of 2.4-D and BAP on protocorm formation in meristematic regions of root and leaf of Miltonia spectabilis moreliana, and to test the effect of sucrose concentrations and pH levels on the growth of protocorms. Root and leaf segments of in vitro-germinated plants were cultured in MS/2 culture medium supplemented with 2.4-D and BAP, and kept in the dark for 210 days. The combination of 3 mg.L-1 2.4-D and 1 or 3 mg.L-1 BAP provided the best results. The concentrations of 15 and 30 g.L-1 sucrose, independently of the pH, were more efficient for the in vitro culture of this species, as they promoted an increase in the number and length of roots, height, and fresh weight of the plantlets. The pH was significant only in combination with 15 g.L-1 sucrose for the length of the largest root and fresh weight.

scholarly journals Clonal Propagation of Phalaenopsis amabilis (L.) BL. cv. 'Golden Horizon' Through In vitro Culture of Leaf Segments

1970 ◽  
Vol 46 (2) ◽  
pp. 163-168 ◽  
Author(s):  
P Sinha ◽  
MAA Jahan
Keyword(s):  
In Vitro Culture ◽  
Natural Environment ◽  
Activated Charcoal ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Huge Amount ◽  
Leafy Shoots ◽  
Half Strength ◽  
Leaf Segments

A protocol was established for mass clonal propagation of Phalaenopsis amabilis cv. 'Golden horizon' through in vitro culture of young leaf segments from mature plant. Explants were cultured on half strength Murashige and Skoog (1/2 MS) medium supplemented with N6-benzyladenine (2.0 mg l-1), a-naphthaleneaceetic acid (0.5 mg l-1), 2% (w/v) sucrose, 10% (v/v) coconut water, 2 g l-1 peptone and 1 g l-1 activated charcoal. Each section of explant produced 15 protocorm-like bodies (PLBs) after 12 weeks of culture. When phytohormone was omitted from the medium and 150 mg l-1 L-glutamone was added PLBs were found to be enlarged with leafy shoots and new PLBs were induced from the base of the old ones. Leafy shoots rooted on half strength MS medium supplemented with 2 g l-1 peptone, 2% (w/v) sucrose, 10% (v/v) CW and 1 g l-1 activated charcoal, where 100% explants were developed into plantlets with roots within 8 weeks. The addition of 2.5 g l-1 banana pulp powder enhanced the number and length of roots. Within the first 32 weeks after initiation of culture about 1500 plantlets as well as a huge amount of PLBs were achieved from a single explant section. The plantlets were acclimatized in natural environment. Key words: Phalaenopsis orchid; Leaf segments; Protocorm-like bodies; Micropropagation DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8182 Bangladesh J. Sci. Ind. Res. 46(2), 163-168, 2011

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens

Effect of antioxidants on in vitro culture of pomegranate cv. Sindhuri

2018 ◽  
Vol 34 (2) ◽  
pp. 311-318
Author(s):  
Ravi Kumar ◽  
◽  
M.L. Jakhar ◽  
Komal Sekhawat ◽  
Swarnlata Kumawat ◽  
...  
Keyword(s):  
In Vitro Culture

In vitro Culture of Human PBMCs

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (3) ◽  
Author(s):  
Santosh Panda ◽  
Balachandran Ravindran
Keyword(s):  
In Vitro Culture ◽  
Human Pbmcs
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