Accumulation of Anthocyanidins Determines Leaf Color of Liquidambar Formosana as Revealed by Transcriptome Sequencing and Metabolism Analysis

Liquidambar formosana is important for its ornamental value in China; it is increasingly used for landscaping and gardening trees due to its diverse leaf colors and seasonal changes. Varieties including either a fixed leaf color, the purplish ‘Fuluzifeng’ (ZF), or seasonal changes in leaf color, the reddish ‘Nanlinhong’ (NLH) have been bred and registered as new plant varieties under the International Union for the Protection of New Plant Varieties (UPOV) system. To gain practical insights into the anthocyanin biosynthetic process, transcriptome sequencing (Illumina) was performed to clarify the metabolic pathways present in the three seasonal changes in leaf colors in NLH and in the springtime purple-red color of ZF. qRT-PCR was used to verify the speculation. Based on the differentially expressed genes and flavonoids analyses, the spring, summer, and autumn leaves of NLH were compared to study the seasonal differences. NLH and ZF were compared to study the formation mechanism of the two leaf colors in spring. Transcriptome sequencing produced a total of 121,216 unigenes from all samples, where 48 unigenes were differentially expressed and associated with the anthocyanidin pathway. The expression levels of LfDFR and LfANS genes corresponded to the accumulation of concentrations of cyanidins in spring (NLHC) and autumn leaves (NLHQ), respectively, with different shades of red. Moreover, the LfF3′5′H gene corresponded to the accumulation of flavonols and delphinidins in purple-red leaves (ZFC). Cyanidin and peonidin were the key pigments in red and dark-red leaves, and purple-red leaves were co-pigmented by cyanidins, pelargonidins, and delphinidins.

SLOW GROWTH IN VITRO CULTURE FOR CONSERVATION OF HANCORNIA SPECIOSA GOMES

Hancornia speciosa Gomes is a fruit species endemic to the Cerrado and coastal plains of Northeast of Brazil, with great economic, nutritional, ecological, and medicinal potential. This study aimed to evaluate the effect of sorbitol and sucrose as osmotic regulators on the in vitro growth of mangabeira, aiming at conservation by slow growth. The explants were obtained from in vitro germinated seedlings and inoculated in MS medium supplemented with sucrose (15 and 30 g L-1) and sorbitol (0, 5, 10 and 15 g L-1). The experimental design was completely randomized with 20 repetitions in a 4 x 2 factorial arrangement (sorbitol x sucrose concentrations). The evaluations were performed at 30, 60 90 and 120 days of incubation. The analyzed variables were number of nodes/budding, number of leaves, leaf abscission, leaf color and survival of explants. The data were statistically analyzed by generalized linear model analysis. The results indicated a significant difference between the osmotic regulators and the culture time for all variables. Sorbitol showed a more pronounced growth-reducing effect than sucrose. The use of 30 g L-1 sucrose combined with 10 or 20 g L-1 sorbitol reduced the growth in a critical way, making it clear that the water stress caused was not tolerated by the plants, negatively interfering in its development. Treatment with 15 g L-1 sucrose combined with 5 g L-1 sorbitol promoted the best result, allowing the conservation of plants for 120 days.

Perbedaan pertumbuhan dan produktivitas varietas bayam hijau dan bayam merah

Bayam termasuk salah satu sayuran terpenting di Indonesia karena paling banyak dikonsumsi setelah kangkung. Bayam hijau dan bayam merah merupakan jenis bayam paling banyak ditanam dan dikonsumsi. Penelitian ini bertujuan untuk mengetahui perbedaan pertumbuhan dan produktivitas varietas bayam hijau dan bayam merah. Penelitian dilakukan pada Februari – April 2021 di Kebun Percobaan Leuwikopo, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, IPB University. Sebanyak 9 varietas bayam hijau (Maryland, Richie, Maestro, Benua, Doly, Khanafiah, Manila, Pacific, White Leaf) dan 4 varietas bayam merah (Mira, Baret Merah, Clara, Aurora) ditanam menggunakan rancangan kelompok lengkap teracak dengan tiga ulangan. Hasil penelitian memperlihatkan bahwa varietas bayam hijau memiliki kandungan persentase warna hijau daun lebih banyak namun memiliki persentase warna biru dan merah yang lebih rendah dibandingkan varietas bayam merah. Hasil penelitian juga memperlihatkan bahwa varietas bayam hijau menghasilkan tinggi tanaman dan produktivitas yang lebih baik dibandingkan varietas bayam merah, namun varietas bayam merah menghasilkan luas daun dan jumlah daun per tanaman saat panen yang lebih baik besar dibandingkan varietas bayam hijau. Varietas White Leaf merupakan varietas bayam yang sangat baik karena memiliki persentase warna hijau daun yang tinggi, luas daun yang besar dan produktivitas yang tinggi. Spinach is one of the most important vegetables in Indonesia because it was the second most consumed after kangkung. Green spinach and red spinach are the most widely grown and consumed types of spinach. This study aimed to determine differences in growth and productivity between varieties of green spinach and red spinach. The research was conducted in February – April 2021 at the Leuwikopo Experimental Field, Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University. A total of nine varieties of green spinach and four varieties of red spinach were planted using a completely randomized block design with three replications. The results showed that the green spinach variety contained a higher percentage of green leaf color but had a lower percentage of blue and red leaf color than the red spinach varieties. The results also showed that the green spinach varieties produced better plant height and productivity than the red spinach varieties, but the red spinach produced better leaf area and a number of leaves per plant at harvest than the green spinach. The White Leaf variety is the excellent spinach variety because it has a high percentage of green leaf color, large leaf area, and high productivity.

Kinetics, modeling and colorimetry of celery leaves dehydration by direct and mixed solar drying

In celery, leaves, roots, and fruit contain a high value in medicinal properties and are used to prepare syrups, tinctures, infusions, or oils; however, its leaves are commonly discarded, wasting their nutritional and medicinal content. The dehydration of these leaves is a conservation option, increasing their shelf life. This study analyzes direct and mixed solar drying (SD and SM) kinetics and their effect on celery leaves. The moisture contents, drying rate, water activity, and colorimetry were obtained. Moreover, the fitting of experimental data to the mathematical models proposed in the literature. The moisture content stabilized at 150 min in the SM at the shortest time with a maximum drying rate of 0.1179 g∙water/g∙ dm∙min. The initial and final water activity was 0.98 and 0.412 in the SM and 0.403 in the SD. The SD better conserved the leaf color, with a total color change (ΔE) of 2.56, while the value obtained with the SM was 5.42. The experimental results of both technologies were better adjusted to the model Two exponential terms with an R² of 0.999. The results show that the solar drying of the celery leaves is feasible, and a quality product is obtained sustainably.

Sistem Pendukung Keputusan Pemilihan Bibit Tanaman Karet Dengan Metode Profile Matching

PT. Asri Jaya had difficulty in selecting the best rubber plant seeds, both for direct planting in the plantations and recommending them to be marketed to rubber plantation companies, because the types of rubber plant seeds in the company had many varieties and varied as well as different ages of seeds. -different such as BPM seeds, IRR seeds, IRC, rubber frame seeds, GT (Gondang Tapen), PB seeds (Prang Besar), and so on. The alternatives used in this research are types of rubber plant seeds, namely BPM seeds (Balai/Pusat Perkebunan Medan, IRR (Indonesian Rubber Research) rubber seeds), IRC rubber seeds (Institute Research of Ceylon), frame rubber seeds, GT rubber seeds (Gondang Tapen) and PB (Prang Besar) rubber seedlings. The criteria used in this study were umbrella shape, petiole position, petiole shape, leaf color, leaf shape, leaf bone, leaf thickness, stem height, and seedling age. which is built using the programming language used in making the system is PHP with MySQL database. To calculate the total value, it must first determine the percentage of core factore and secondary factore. In this case the core factore is determined with a percentage value of 60% considering the main factor is an the most dominant aspect or criteria for assessing the selection of rubber plant seeds, the percentage value is greater than the pe value the percentage for the secondary factor is 40%.

Supplementary Far-Red and Blue Lights Influence the Biomass and Phytochemical Profiles of Two Lettuce Cultivars in Plant Factory

Three different LED spectra (W: White light; WFR: W + far-red light; WB: W + blue light) with similar photosynthetic photon flux density (PPFD) were designed to explore the effects of supplementary far-red and blue lights on leaf color, biomass and phytochemicals of two cultivars of red-leaf lettuce (“Yanzhi” and “Red Butter”) in an artificial lighting plant factory. Lettuce plants under WB had redder leaf color and significantly higher contents of pigments, such as chlorophyll a, chlorophyll b, chlorophyll (a + b) and anthocyanins. The accumulation of health-promoting compounds, such as vitamin C, vitamin A, total phenolic compounds, total flavonoids and anthocyanins in the two lettuce cultivars were obviously enhanced by WB. Lettuce under WFR showed remarkable increase in fresh weight and dry weight; meanwhile, significant decreases of pigments, total phenolic compounds, total flavonoids and vitamin C were found. Thus, in the plant factory system, the application of WB can improve the coloration and quality of red leaf lettuce while WFR was encouraged for the purpose of elevating the yield of lettuce.

Review: the effect of light on the key pigment compounds of photosensitive etiolated tea plant

Background Light is the ultimate energy source of plant photosynthesis, which has an important impact on the growth, development, physiology and biochemistry of tea plant. Photosensitive etiolated tea plant belongs to a kind of colored leaf plant, which is a physiological response to light intensity. Compared with conventional green bud and leaf of tea plant, the accumulation of pigment compounds (chlorophyll and carotenoids, etc.) closely related to a series of reactions of photosynthesis in photosensitive etiolated tea plant is reduced, resulting in the difference of leaf color of tea. This specific tea resource has high application value, among which high amino acid is one of its advantages. It can be used to process high-quality green tea with delicious taste and attractive aroma, which has been widely attention. The mechanism of the color presentation of the etiolated mutant tea leaves has been given a high topic and attention, especially, what changes have taken place in the pigment compounds of tea leaves caused by light, which makes the leaves so yellow. At present, there have been a lot of research and reports. Purpose of the review We describe the metabolism and differential accumulation of key pigment compounds affecting the leaf color of photosensitive etiolated tea that are triggered by light, and discuss the different metabolism and key regulatory sites of these pigments in different light environments in order to understand the “discoloration” matrix and mechanism of etiolated tea resources, answer the scientific question between leaf color and light. It provides an important strategy for artificial intervention of discoloration of colored tea plant. Conclusion The differential accumulation of pigment compounds in tea plant can be induced phytochrome in response to the change of light signal. The synthesis of chlorophyll in photoetiolated tea plants is hindered by strong light, among which, the sites regulated by coproporphyrinogen III oxidase and chlorophyllide a oxidase is sensitive to light and can be inhibited by strong light, resulting in the aggravation of leaf etiolation. The phenomenon can be disappeared or weakened by shading or reducing light intensity, and the leaf color is greenish, but the increase of chlorophyll-b accumulation is more than that of chlorophyll-a. The synthesis of carotenoids is inhibited strong light, and high the accumulation of carotenoids is reduced by shading. Most of the genes regulating carotenoids are up-regulated by moderate shading and down-regulated by excessive shading. Therefore, the accumulation of these two types of pigments in photosensitive etiolated tea plants is closely related to the light environment, and the leaf color phenotype shape of photosensitive etiolated tea plants can be changed by different light conditions, which provides an important strategy for the production and management of tea plant.