scholarly journals Laboratory Evaluation of an Entomopathogenic Fungus, Isaria fumosorosea wize pa208 against Two-spotted Spider Mite, Tetranychus cucurbitacearum (SAYED)

2017 ◽  
Vol 9 (3) ◽  
pp. 1-6
Author(s):  
El-Kawas G. ◽  
Nabil A. ◽  
Kalmosh Sh. ◽  
Hussein M.
Keyword(s):  
Entomopathogenic Fungus ◽  
Spider Mite ◽  
Laboratory Evaluation ◽  
Isaria Fumosorosea ◽  
Two Spotted Spider Mite

The pathogenicity of entomopathogenic fungus Acremonium hansfordii to two-spotted spider mite, Tetranychus urticae and predatory mite Neoseiulus barkeri

2018 ◽  
Vol 23 (11) ◽  
pp. 2173
Author(s):  
Su-Qin Shang ◽  
Yao-Nian Chen ◽  
Ying-Lu Bai
Keyword(s):  
Tetranychus Urticae ◽  
Entomopathogenic Fungus ◽  
Spider Mite ◽  
Crop Yields ◽  
Predatory Mite ◽  
Experimental Conditions ◽  
Neoseiulus Barkeri ◽  
Preoviposition Period ◽  
Two Spotted Spider Mite ◽  
F1 Generation

The two-spotted spider mite, Tetranychus urticae, is an important mite pest worldwide. It often leads to reduced crop yields or poor marketability of the produce and has already developed resistance to many acaricides. In this study, the pathogenicity of the entomopathogenic fungus (EPF), Acremonium hansfordii, to T. urticae and its side effects on the predatory mite Neoseiulus barkeri were compared. The toxicity of A. hansfordii was evaluated on T. urticae at 1.0×104, 1.0×106, 1.0×108 conidia/mL concentrations and predatory mite N. barkeri at 1.0×108 conidia/mL under experimental conditions at 25±1°C, 75±5% RH and 16L: 8D photoperiod. The effects of EPF on the development of the F0 and F1 generation of predatory mites were also assessed at 1.0×108 conidia/mL concentration. The results showed that the corrected mortality of T. urticae at three tested concentrations were 36.67%, 53.67% and 66.33% within 11d, and the median lethal time were 14.39 d, 9.84d and 7.99d, respectively. The corrected mortality of N. barkeri was only 3.9% after 11d at 1×108 conidia/mL concentration. Some hyphae of A. hansfordii were detected on the surface of treated T. urticae but not on N. barkeri body after 7d. After N. barkeri females were treated with 1.0×108 conidia/mL, the preoviposition period of the F0 generation was prolonged 1.56 times and the other life history parameters have no significant differences; similar patterns were also shown in the F1 generation. A. hansfordii showed strong pathogenicity against T. urticae but did not significantly adversely influence N. barkeri. Therefore, the combination of A. hansfordii and N. barkeri has the potential to be used for the control of T. urticae in the future. 

scholarly journals Myco-metabolites as biological control agents against the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae)

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Mohamed E. Osman ◽  
Amany A. Abo Elnasr ◽  
Mohamed A. Nawar ◽  
Gohyza A. Hefnawy
Keyword(s):  
Secondary Metabolites ◽  
Tetranychus Urticae ◽  
Spider Mite ◽  
Laboratory Evaluation ◽  
Biological Control Agents ◽  
Liquid Chromatography Mass Spectrometry ◽  
Agricultural Pests ◽  
Two Spotted Spider Mite ◽  
Tetranychus Urticae Koch ◽  
Promising Source

Abstract Fungi are a promising source for bioactive secondary metabolites against various agricultural pests. Soil samples were collected from the rhizosphere of various plants at El-Khatatba, Egypt, in May 2016. Sixty-two fungal isolates were locally isolated and screened against Tetranychus urticae Koch (Acari: Tetranychidae). Four fungal strains, showed potent control activities, were morphologically identified. Laboratory evaluation of the crude extracts of the selected strains, Aspergillus melleus, A. terreus, Emericella nidulans, and Chaetomium globosum, exhibited LC50 values of 10.27, 33.05, 14.68, and 22.40 mg/ml against females of T. urticae, respectively. Correspondent LC50 values that exhibited on eggs were 8.81, 23.17, 11.66, and 11.05 mg/ml. Consequently, secondary metabolites of A. melleus were separated by liquid chromatography/mass spectrometry. Compounds separated from the active fraction were identified as mellamide, ochratoxin C, nodulisporic acid, 7-Oxocurvularin, and 6-(4′-hydroxy-2′-methyl phenoxy)-(−)-(3R)-mellein. Obtained secondary metabolites are promising sources for biopesticides to be used in bio-rationale control of T. urticae.

Tomato Carrying MI-1.2 Gene as a Host-Plant to the Two-Spotted Spider Mite (Tetranychus urticae Koch): Results of Laboratory Evaluation

2010 ◽  
Vol 72 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Monika Godzina ◽  
Małgorzata Kiełkiewicz ◽  
Katarzyna Szymczykiewicz
Keyword(s):  
Host Plant ◽  
Tetranychus Urticae ◽  
Spider Mite ◽  
Reproductive Potential ◽  
Laboratory Evaluation ◽  
Population Parameters ◽  
Tomato Plants ◽  
Two Spotted Spider Mite ◽  
Short Time ◽  
Tetranychus Urticae Koch

Tomato CarryingMI-1.2Gene as a Host-Plant to the Two-Spotted Spider Mite (Tetranychus urticaeKoch): Results of Laboratory EvaluationMi-1.2gene contributes to the resistance of tomato plants against some species of herbivores (i.e. nematodes, aphids) that are sap-feeders. In this study, two genotypes of tomato (Solanum lycopersicumL. syn.Lycopersicon esculentumMill.) plants with dominant (Motelle cv) and recessive (Moneymaker cv)Mi-1.2alleles were evaluated as a host-plant to the two-spotted spider mite (Tetranychus urticaeKoch, Acari: Tetranychidae). Plants were grown under glasshouse conditions. The effect of tomato genotypes onT. urticaebionomy was estimated on cut leaflets in controlled photoperiod, temperature and humidity (L/D=16/8 h; D/N temp.=23/20°C; RH=70%). Results of laboratory evaluation showed that both the bionomy and population parameters ofT. urticaereached a very similar or even higher value on plants with dominantMi-1.2allele than on plants with recessive one. A relatively high value ofT. urticaeparameters on plants resistant to root knot nematodes indicates that the mite has potential to develop rapidly. This allows the mite population to reach the density causing marked damage to plant in a short time. It is concluded that the presence ofMi-1.2gene product in tomato plants does not significantly affect the reproductive potential ofT. urticae.Therefore,Mi-1.2gene cannot be a satisfying source of plant resistance to this mite-herbivore generalist.

scholarly journals Susceptibility of Tetranychus urticae koch. (Acari: Tetranychidae) to isolates of entomopathogenic fungus Beauveria bassiana

2010 ◽  
Vol 25 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Slavimira Draganova ◽  
Spaska Simova
Keyword(s):  
Tetranychus Urticae ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Spider Mite ◽  
Probit Analysis ◽  
Average Value ◽  
Lethal Time ◽  
Two Spotted Spider Mite ◽  
Tetranychus Urticae Koch ◽  
Fungus Beauveria Bassiana

Bioassays with five isolates of the entomopathogenic fungus Beauveria bassiana (Bals.- Criv.) Vuillemin were conducted under laboratory conditions with a goal to estimate their virulence to the two-spotted spider mite Tetranychus urticae Koch. (Acari: Tetranychidae). Common bean plants with mites were treated by spraying conidial suspensions of isolates at concentrations of 106, 107 and 108 conidia/ml. Lethal effects of the fungal isolates were evaluated as percentages of cumulative daily mortality due to mycoses, corrected for mortality in the control variant. Virulence of the isolates was estimated based on values of the median lethal time (LT50) calculated by probit analysis for the variants treated with conidial suspensions at the concentration of 106 conidia/ml. The two-spotted spider mite was found susceptible to the examined isolates of the entomopathogenic fungus B. bassiana. Mycosis caused to T. urticae by the B. bassiana isolates 444 Bb and 445 Bb had fast lethal effect after treatment with conidial suspensions even at the concentration of 106 conidia/ ml. The mean mortality values of host individuals were 83.78 ? 3.62% and 68.49 ? 4.28% on the first day, respectively, and up to 100% in both variants on the fourth day. The isolates 426 Bb, 444 Bb and 445 Bb of B. bassiana were highly virulent to two-spotted spider mites with values of the median lethal time varied within overlapped narrow confidence intervals from 0.122 to 1.084 days (average value 0.162 days), from 0.117 to 1.398 days (average value 0.146 days) and from 0.106 to 1.162 days (average value 0.131 days), respectively. Significant differences regarding virulence of the three isolates at p-level < 0.05 could not been proved. The other two examined isolates were distinctly less virulent to T. urticae than these three B. bassiana isolates.

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