Evaluation of indigenous plants’ extracts for mosquitocidal activity against different stages of Culex quinquefasciatus say (Diptera: Culicidae)

Being vector of West Nile Virus and falariasis the control of Culex quinquefasciatus is likely to be essential. Synthetic insecticide treatment is looking most effective for vectors mosquito control. However, these products are toxic to the environment and non-target organisms. Consequently, ecofriendly control of vectors mosquito is needed. In this regard botanical insecticide is looking more fruitful. Therefore, the present research aimed to investigate the effectiveness of methanolic extract and various fractions, including, n-hexane, ethyl-acetate, chloroform, and aqueous fraction, obtained from methanolic extract of Ailanthus altissima, Artemisia scoparia, and Justicia adhatoda using separating funnel against larval, pupal, and adult stages of Culex quinquefasciatus. The larvae and pupae of Culex quinquefasciatus were exposed to various concentrations (31.25-1000 ppm) of methanolic extract and its fractions for 24 hours of exposure period. For knock-down bioassay (filter paper impregnation bioassay) different concentration of the methanolic extract and its various fractions (i.e. 0.0625, 0.125, 0.25, 0.5 and 1mg/mL) were applied for 1 hour exposure period. The results were statistically analysed using standard deviation, probit analysis, and linear regression. The R2 values of larvae, pupae, and adult range from 0.4 to 0.99. The values of LC50 (concentration causing 50% mortality) for late 3rd instar larvae after 24 hours exposure period range from 93-1856.7 ppm, while LC90 values range from 424 -7635.5ppm. The values of LC50for pupae range form 1326.7-6818.4ppm and and values of LC90 range from 3667.3-17427.9ppm, respectively. The KDT50 range from 0.30 to 2.8% and KDT90 values range from1.2 to 110.8%, respectively. In conclusion, Justicia adhatoda may be effective for controlling populations of vector mosquito.

Effectiveness of Cordyceps sp. and Beauveria Bassiana Against Large Cabbage-heart Caterpillar, Crocidolomia Pavonana Fabricius (Lepidoptera: Crambidae)

Crocidolomia pavonana Fabr. (Lepidoptera: Crambidae) is a major pest on various cabbage crops in Indonesia. Various insecticides have been used to control this insect pest including several entomopathogenic fungi, and this cabbage caterpillar has become resistant to various insecticides. In order to develop insecticides from entomopathogenic fungi, species or varieties from natural area such as forest are needed. A bioassays of Cordyceps sp. and Beauveria bassiana has been carried out in the laboratory. Inoculum of Cordyceps sp. obtained from the Dipterocarpaceae forest in South Kalimantan, Indonesia recovered from dead ants by the mycelium of Cordyceps sp. Four concentration of Cordyceps sp. and Beauveria bassiana inoculum and tween80 as a control were tested against 3rd instar larvae of C. pavonana with five replications. Field efficacy trial was carried out in a screen house (8 x 12 M2) using Chinese cabbage plants (Brassica juncea) aged 28 days after sowing, with a spacing of 0.4 M between rows. Three formulation of Cordyceps sp. and tween80 as a control were used with 6 replications. The result of the bioassays in the laboratory showed that Cordyceps sp. with concentration of 1 x 107 conidia mL-1 was able to give 100% mortality od C. pavonana larvae, while B. bassiana with the same concentration caused morality of C. pavonana larvae of 98.33%, although statistically not significantly different. The results of probit analysis also showed that LC90 Cordyceps sp. against cabbage caterpillar larvae was 1.57 x 106 conidia mL-1, while B. bassiana required a concentration of 2.6 x 106 conidia mL-1 to reach the LC90 value. The results of filed trial showed that the formulation of Cordyceps sp. 1 x 107 conidia mL-1 using palm oil cooking oil was able to give an average mortality of 86.7%, and significantly higher (P<0.05) than the other treatment. The results of this study indicate that the inoculum of Cordyceps sp. from Dipterocarpaceae forest is very promising to be developed as an insecticide to control C. pavonana in cabbage

Antimalarial Activity of Ethanol Extract of Kelakai Leaves (Stenochlaena palustris) to Parasitemia and Splenomegaly in BALB/c Mice Infected with Plasmodium berghei ANKA

Introduction: Malaria is one of global health problems. Splenomegaly is one of malaria symptoms. Antimalarial drug resistance had been reported. Alternative treatment is by using traditional medicinal plants such as kelakai (Stenochlaena palustris). Kelakai contains alkaloid and flavonoid which had been reported to have antimalarial activity. The aim of this study was to discover antimalarial activity of ethanol extract of kelakai leaves to parasitemia and splenomegaly of Plasmodium berghei ANKA in infected BALB/c mice.Methods: This study was based on a modified Peter test using BALB/c mice infected with P. berghei ANKA treated with ethanol extract of kelakai leaves, with chloroquine diphosphate as a positive control. The negative control was P. berghei ANKA infected mice without any additional treatment. Administration of ethanol extract of kelakai leaves was performed for 4 days with a serial doses of 100, 10, and 1 mg/kg body weight. The positive control was given chloroquine diphosphate 20 mg/kg body weight. Parasitemia was observed daily prior to the calculation of the percentage of parasite growth and parasite growth inhibition. At the end of the test, the mice were sacrificed and spleens were isolated to measure their sizes. Probit analysis was performed to obtain ED50 to find the effect of extract in parasite killing by 50%. Spearman test was performed to analyze the correlation of doses of extract and splenomegaly.Results: Parasitemia growth inhibition was directly proportional to the dose. Higher parasitemia inhibition was obtained at higher doses and vice versa. Result of probit analysis showed an ED50 was 77.05 mg/kg body weight. Statistical analysis resulted in insignificant correlation between doses and splenomegaly p = 1.0 (significancy < 0.05).Conclusion: Ethanol extract of kelakai leaves possessed good antimalarial activity and there was no correlation between extract doses and splenomegaly in Plasmodium berghei ANKA-infected mice.

The radicle emergence test and storage longevity of cucurbit rootstock seed lots

A study was carried out to test whether a single count of radicle emergence (RE test) would correlate with the storage potential of seed lots of cucurbit (C. maxima &times; C. moschata) rootstock cultivars. The RE test was performed by counting radicle emergence percentage between 26 and 100 hours of germination. Seed longevity was determined by storage at 75% relative humidity at 35&deg;C over 100 days, with seed survival curves constructed based on normal germination percentages. Ki (estimated initial viability in probits), &sigma; (standard deviation of the normal distribution of seed death in time) and p50 (time for viability to fall to 50%) were determined through probit analysis. Correlation analysis showed that RE counts particularly between 30 and 34 hours were highly (P &lt; 0.01) correlated with normal germination percentage, Ki and p50. It can be concluded that RE tests can be used as an indicator of subsequent seed lot longevity in cucurbit rootstock seed cultivars.

on Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae)

A study was undertaken on the ovicidal and larval repellent activity of Tagetes erecta leaf and flower extracts on Rhipicephalus sanguineus (Latreille, 1806), an important tick species in the world from an economic and medical point of view. Ethanol and methanol extracted plant products tested against the eggs and larvae of R. sanguineus indicated that the ethanol extract of flower had maximum ovicidal activity (86.1%), followed by the ethanol extract of leaf (75%) at 25 mg ml-1 concentration. In all analyses, the homogeneity of variance was significant. The probit analysis clearly indicated that the ethanol extract of the flower has a higher ability to kill the eggs. In the case of larval repellency tested, both extracts of leaf showed the highest repellency (83%) at 2.5 mg ml-1. Significant tick repellency (> 90%) was found in both methanol and ethanol extracts of flower at 2.5 mg ml-1. GC-MS analysis of extracts revealed the presence of bioactive insecticidal compounds such as yangambin, cyclohexane and neophytadine.

Selection for and Analysis of UV-Resistant Cryptophlebia Leucotreta Granulovirus-SA as a Biopesticide for Thaumatotibia leucotreta

Cryptophlebia leucotreta granulovirus-SA (CrleGV-SA) is used as a commercial biopesticide for the false codling moth, Thaumatotibia leucotreta, in citrus and other crops. The virus is sensitive to UV irradiation from sunlight, which reduces its efficacy as a biopesticide in the field. We selected a UV-resistant CrleGV-SA isolate, with more than a thousand-fold improved virulence compared to the wild-type isolate, measured by comparing LC50 values. CrleGV-SA purified from infected T. leucotreta larvae was exposed to UV irradiation under controlled laboratory conditions in a climate chamber mimicking field conditions. Five cycles of UV exposure, followed by propagating the virus that retained infectivity in vivo with re-exposure to UV, were conducted to isolate and select for UV-resistant virus. Serial dilution bioassays were conducted against neonates after each UV exposure cycle. The concentration-responses of the infectious UV-exposed virus populations were compared by probit analysis with those from previous cycles and from the original CrleGV-SA virus population. NGS sequences of CrleGV-SA samples from UV exposure cycle 1 and cycle 5 were compared with the GenBank CrleGV-SA sequence. Changes in the genomes of infective virus from cycles 1 and 5 generated SNPs thought to be responsible for establishing UV tolerance. Additional SNPs, detected only in the cycle 5 sequence, may enhance UV tolerance and improve the virulence of the UV-tolerant population.

Toxicity and Antifeedant Activity of Calotropis gigantea L. Leaf Extract Against Plutella xylostella L. (Lepidoptera: Plutellidae)

Many parts of the Calotropis gigantea plant are known to contain bioactive compounds, but leaves contain the most. This study aimed to determine the toxicity and antifeedant activity of C. gigantea leaves against Plutella xylostella. The study was carried out from November 2019 to July 2020. Toxicity was tested using the leaf dipping and spraying methods. Antifeedant activity was tested using a no-choice test and a choice test. Identification of the compound composition of the leaf extract of C. gigantea was carried out at the Integrated Research and Testing Laboratory, Gadjah Mada University. Extract toxicity data obtained were analyzed by Probit analysis. The results showed that the antifeedant activity of C. gigantea leaf extract a no-choice and with choice at each concentration had a significant effect on the consumption of P. xylostella larvae rations. The toxicity (LC50) of the leaf extract of C. gigantea to P. xylostella by the dipping method was 2,958 µgl-1 while the spraying application was 3.944 µgl-1. The composition of chemical compounds contained in the leaf extract of C. gigantea is saponins, alkaloids, flavonoids, tannins, phenols, terpenoids. With the composition of these chemical compounds, the leaf extract of C. gigantea has the potential as a source of vegetable insecticide compounds against P. xylostella.

Analysis Socioeconomic Influence on the Utilization of Health Service in the Inpatient Room of Faisal Islamic Hospital Makassar

Hospitals in carrying out their role cannot be separated from problems, both from within and from outside that can interfere with the mechanism of work of the hospital in providing health services for the community. Faisal Islamic Hospital Makassar is one of the private hospitals in the city of Makassar which in the last 3 years has decreased the value of BOR. The purpose of this study was to find out the socio-economic influence on the utilization of health services in the inpatient room of Faisal Islamic Hospital Makassar. This research is a cross-sectional study. A total of 99 people were sampled in the inpatient room of Faisal Islamic Hospital Makassar by taking a sample using the purposive sampling method. Data is collected by conducting interviews with respondents. Data processing is done by frequency distribution analysis and univariate and multivariate analysis with the Binary Regression approach namely logit and probit analysis. Based on the results of the study, it was obtained that socioeconomic variables that have an influence on the utilization of health services in the Faisal Islamic Hospital Makassar is the ownership of health insurance with the results of p-value< 0.1 is logit 0.299 and probit of 0.000. For the government to evaluate the ownership of health insurance, because there are still respondents who do not have health insurance where this is not in accordance with the objectives of the National Health Insurance program.

Preparation and Optimization of Peppermint (Mentha Pipertia) Essential Oil Nanoemulsion with Effective Herbal Larvicidal, Pupicidal, and Ovicidal Activity against Anopheles Stephensi

Objectives: The Plasmodium parasite is transmitted directly to humans through the Anopheles mosquito bite and causes vector-borne Malaria disease, which leads to the transmission of the disease in Southeast Asia, including India. The problem of persistent toxicity, along with the growing incidence of insect resistance, has led to the use of green pesticides to control the spread of the disease in a cost-effective and environment-friendly manner. Based on this objective, this work investigated the larvicidal, pupicidal, and ovicidal activity of Mentha pipertia using a natural nanoemulsion technique. Methods: GC-MS characterized essential oils of Mentha pipertia leaves were formulated as a nanoemulsion for herbal larvicidal, pupicidal, and ovicidal activities. Size of the nanoemulsion was analyzed by photon correlation spectroscopy. The herbal activities against Anopheles Stephensi of nanoemulsion were evaluated in terms of the lethal concentration for 50% (LC50) and 90% (LC90) to prove low cost, pollution free active effective formulation. Results: Chiral, keto, and alcohol groups attached Mentha pipertia leaves essential oil nanoemulsions demonstrated good results in the larvicidal probit analysis, with values of LC50=09.67 ppm and LC90=20.60 ppm. Activity results of the most stable nano formulation with 9.89 nm size showed a significant increase when compared to the bulk. Conclusion: The nanoemulsion of Mentha pipertia leaves can be a promising eco-friendly widely available, low-cost herbicide against the Anopheles mosquito.

Efficacy of Essential Oils from Ocimum kenyense as a Biopesticide against Aphis gosypii, Thrips tabaci and Bemisia tabaci.

Natural sources of biopesticides such as plants and microorganisms are widely employed for pest management globally. Over the last five decades, the use of biopesticides has been explored as a safer alternative to synthetic pesticides. This study determined the repellence and contact toxicity of essential oils from Ocimum kenyense plant against Thrips tabaci, Bemisia tabaci, and Aphis gosypii. The essential oils (Eos) were extracted from dry leaves by hydrodistillation using the Clevenger apparatus for 8 hours. The composition of essential oils was qualitatively and quantitatively analysed using GC-MS, while ATR-FTIR was used to determine the functional groups. The major compound ion in the Eos was 1,8-Cionele while the major phytochemical identified was Eucalyptol Bioassay of the crude extracts were carried out on T. tabaci, B. tabaci, and A. gosypii. Contact toxicity and repellency tests against mixed-sex adult pests were carried out on T. tabaci, B. tabaci, and A. gosypii. In contact toxicity, five different concentrations were prepared and each was replicated five times. Acetone was used as a negative control, while permethrin, a commercial chemical pesticide was used as a positive control. The LD50 of the essential oils was determined by Probit analysis SPSS version 26.0. The response to the treatments was observed after 24 hours by using a blunt object probing and the pests that did not respond were counted as dead. It was found that essential oils from O. kenyense had the lowest LD50 of 0.127 µL against A. gosypii. This implies that O. kenyense can be used as a contact toxicant against A. gosypii. Repellency tests were carried out at four different concentrations. N, N-Diethyltoluamide (DEET) repellent from a commercial mosquito repellent was used to compare the effectiveness of the essential oils against commercial repellents. The repellency test responses were observed after 1 hour and the data was used to determine the correlation between different levels of concentration of Eos and percentage repellency of Eos of O. kenyense leaves carried out at α=0.05. There was a high correlation of 1.000 between the increase in concentration of Eos and repellency against A. gosypii, T. tabaci and B. tabaci. The results were statistically significant and (P<0.05, α=0.05). Therefore, essential oils from O. kenyense can be used as a contact toxicant biopesticide against A. gosypii and a repellent biopesticide against A. gosypii, T. tabaci and B. tabaci.