Growing plant tissue cultures under specific light quality treatments (e.g., white, red, blue, yellow, or orange light) has been conducted to study altered morphogenesis. Characteristic differences in leaf shape, rooting, axillary shooting and fresh weights may be obtained depending on the light quality treatments employed. However, light quality treatments also results in a reduction of available light intensity provided to the culture which can be detrimental. Long-term treatments (i.e., 8 weeks) with certain light treatments may result in severe stunting in growth or even culture death. This situation can be corrected by increasing the light intensity for each light quality treatment employed or reducing the time of treatment exposure. As an alternative, this study was conducted to determine if coupling colored light treatments with enhanced ultra-high CO2 levels (e.g., 10,000 Lμliter–1 CO2) could result in growth rates comparable to that occurring in white light treatments. The growth results of employing several species (i.e. lettuce, thyme, citrus) shoot cultures under various light quality treatments with and without ultra-high CO2 levels are presented. Ultra-high CO2 levels enhanced culture growth regardless of the light treatment employed. In some cases, cultures that were given ultra-high CO2 levels with several light treatments exhibited growth rates that exceeded that obtained from white light treatments. Based on these results, ultra-high CO2 levels may play a role in testing and obtaining a beneficial effect from light quality treatments on plant tissue cultures.
Influence of light quality on growth and secondary metabolite in tissue cultures of Gardenia jasmonides, Variegata
