scholarly journals Automatic Measurement of the Red Cell Oxygen Dissociation Curve Identical with the Whole Blood Curve.

1999 ◽  
Vol 49 (4) ◽  
pp. 379-387 ◽  
Author(s):  
Abdul Hassan MOHAMMED MAWJOOD ◽  
Kiyohiro IMAI
Keyword(s):  
Whole Blood ◽  
Automatic Measurement ◽  
Red Cell ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation

Red-cell 2,3-diphosphoglycerate in patients with hyperthyroidism

1980 ◽  
Vol 93 (4) ◽  
pp. 424-429 ◽  
Author(s):  
J. L. Alvarez-Sala ◽  
M. A. Urbán ◽  
J. J. Sicilia ◽  
A. J. Diaz Fdez ◽  
F. Fdez Mendieta ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Control Group ◽  
Red Cell ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
The Right ◽  
Serum Thyroid Hormones ◽  
Hyperthyroid Patients ◽  
2,3 Dpg

Abstract. In 21 hyperthyroid female patients studied on 29 occasions, high levels of red-cell 2,3-diphosphoglycerate (2,3-DPG) have been found (5.75 ± 0.7 mm) which, compared to a euthyroid control group (4.88 ± 0.4 mm), could not be accounted for by differences in haematocrit, haemoglobin or phosphataemia. A significant correlation was found (P < 0.05) between serum thyroid hormones and the 2,3-DPG concentration in the hyperthyroid patients. Eight of these patients were reexamined after treatment and normalization of thyroid function, showed a regression to normal 2,3-DPG values (4.81 ± 0.6 mm) which could not be attributed to variations in haematocrit, haemoglobin or phosphataemia either. We therefore deduce that the shift to the right in the haemoglobin oxygen dissociation curve observed in patients of this type may be due to an increase in the red-cell 2,3-DPG content.

MODIFICATION OF THE HAEMOGLOBIN OXYGEN DISSOCIATION CURVE IN WHOLE BLOOD BY A COMPOUND WITH DUAL ACTION

The Lancet ◽  
1984 ◽  
Vol 324 (8393) ◽  
pp. 15-16 ◽  
Author(s):  
R.M. Hyde ◽  
D.J. Livingstone ◽  
R.A. Patterson ◽  
J.F. Batchelor ◽  
W.R. King
Keyword(s):  
Whole Blood ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
Dual Action

The Bohr effect related to blood and erythrocyte pH

1963 ◽  
Vol 205 (2) ◽  
pp. 337-340 ◽  
Author(s):  
Peter Hilpert ◽  
Renate Gislinde Fleischmann ◽  
Doris Kempe ◽  
Heinz Bartels
Keyword(s):  
Oxygen Pressure ◽  
Ph Value ◽  
Oxygen Affinity ◽  
Bohr Effect ◽  
Red Cell ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
Constant Cell ◽  
The Right

The Bohr effect of the blood and the red cell hemolysates of adult and newborn humans, goats and kids, and sheep and lambs were determined and the physiological significance is discussed. Similar determinations were made on blood from an African elephant, yak, camel, Dybowski deer, and llama. The strong displacement to the right of the oxygen dissociation curve which occurs in kids and lambs during the first 5 days of life can be largely explained by a change in the pH value within the erythrocytes. When the oxygen affinity is expressed by the oxygen pressure necessary for half saturation (T50) at a constant cell pH, considerable differences exist between species.

scholarly journals Continuous Determination of the Oxygen Dissociation Curve for Whole Blood

1975 ◽  
Vol 21 (12) ◽  
pp. 1747-1753 ◽  
Author(s):  
Luigi Rossi-Bernardi ◽  
Massimo Luzzana ◽  
Michele Samaja ◽  
Mario Davi ◽  
Daniela DaRiva-Ricci ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Whole Blood ◽  
Circulatory System ◽  
Oxygen Electrode ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Total Oxygen ◽  
Oxygen Dissociation ◽  
Continuous Determination

Abstract We report here the development of a new method that allows continuous determination of the oxygen dissociation curve for microsamples (600 µl) of whole blood under conditions of pH, pco2, methemoglobin concentration, and 2,3-diphosphoglycerate content closely approaching those found in the circulatory system. The method consists of gradually oxygenating a blood sample by adding H2O2 in the presence of catalase (EC 1.11.1.6), to produce the reaction H2O2 → H2O + ½ O2. Because the total oxygen content of blood can be derived from the known rate of H2O2 addition and the po2 is determined in the liquid phase by an oxygen electrode, the two functions (total O2 content) and (% oxygen saturation) vs. po2 are simple to calculate. pco2 and pH are controlled by adding base simultaneously with the gradual oxygenation of blood. The method described thus avoids the direct measurement of oxygen saturation of whole blood.

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