Fed Batch Fermentation and Purification Strategy for High Yield Production of Brucella melitensis Recombinant Omp 28 kDa Protein and its Application in Disease Diagnosis

2013 ◽  
Vol 20 (7) ◽  
pp. 731-740 ◽  
Author(s):  
Karothia B.S. ◽  
Athmaram T.N. ◽  
Thavaselvam D. ◽  
Kumar Ashu ◽  
Sapna Tiwari ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Brucella Melitensis ◽  
Disease Diagnosis ◽  
High Yield ◽  
Fed Batch ◽  
Yield Production ◽  
Fed Batch Fermentation ◽  
High Yield Production

Development of a Simple Fed-Batch Process for the High-Yield Production of Recombinant Shiga Toxin B-Chain Protein

2012 ◽  
Vol 19 (2) ◽  
pp. 228-237 ◽  
Author(s):  
Kulbhushan Sharma ◽  
Pallavi Gupta ◽  
Anil Kumar Singh ◽  
N. Gopalan ◽  
Ram Kumar Dhaked ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Batch Process ◽  
High Yield ◽  
Fed Batch ◽  
Toxin B ◽  
Yield Production ◽  
High Yield Production

scholarly journals Production of L (+) Lactic Acid by Lactobacillus casei Ke11: Fed Batch Fermentation Strategies

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 151
Author(s):  
Paola Monteiro de Oliveira ◽  
Larissa Provasi Santos ◽  
Luciana Fontes Coelho ◽  
Paulo Marcelo Avila Neto ◽  
Daiane Cristina Sass ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Batch Fermentation ◽  
Lactobacillus Casei ◽  
Culture Media ◽  
Feeding Strategy ◽  
High Yield ◽  
Feeding Strategies ◽  
Efficient Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Lactic acid and its derivatives are widely used in pharmaceutical, leather, textile and food industries. However, until now there have been few systematic reports on fed-batch fermentation for efficient production and high concentration of l-lactic acid by lactic acid bacteria. This study describes the obtainment of L (+) lactic acid from sucrose using the Lactobacillus casei Ke11 strain through different feeding strategies using an accessible pH neutralizer such as CaCO3. The exponential feeding strategy can increase lactic acid production and productivity (175.84 g/L and 3.74 g/L/h, respectively) with a 95% yield, avoiding inhibition by high initial substrate concentration and, combined with the selected agent controller, avoids the cellular stress that could be caused by the high osmotic pressure of the culture media. The purification of the acid using charcoal and celite, followed by the use of a cation exchange column proved to be highly efficient, allowing a high yield of lactic acid, high removal of sugars and proteins. The described process shows great potential for the production of lactic acid, as well as the simple, efficient and low-cost purification method. This way, this work is useful to the large-scale fermentation of L. casei Ke11 for production of l-lactic acid.

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