The P-MAPA immunomodulator partially prevents apoptosis induced by Zika virus infection in THP-1 cells

2020 ◽  
Vol 21 ◽  
Author(s):  
Morganna C. Lima ◽  
Elisa A. N. Azevedo ◽  
Clarice N. L. de Morais ◽  
Larissa I. O. de Sousa ◽  
Bruno M. Carvalho ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Virus Infection ◽  
Virus Replication ◽  
Zika Virus ◽  
Mammalian Cells ◽  
Caspase 3 ◽  
Neurological Complications ◽  
Zika Virus Infection

Background: Zika virus is an emerging arbovirus of global importance. ZIKV infection is associated with a range of neurological complications such as the Congenital Zika Syndrome and Guillain Barré Syndrome. Despite the magnitude of recent outbreaks, there is no specific therapy to prevent or to alleviate disease pathology. Objective: To investigate the role of P-MAPA immunomodulator in Zika-infected THP-1 cells. Methods: THP-1 cells were subjected at Zika virus infection (Multiplicity of Infection = 0.5) followed by treatment with P-MAPA for until 96 hours post-infection. After that, the cell death was analyzed by annexin+/ PI+ and caspase 3/ 7+ staining by flow cytometry. In addition, the virus replication and cell proliferation were accessed by RT-qPCR and Ki67 staining, respectively. Results: We demonstrate that P-MAPA in vitro treatment significantly reduces Zika virus-induced cell death and caspase-3/7 activation on THP-1 infected cells, albeit it has no role in virus replication and cell proliferation. Conclusions: Our study reveals that P-MAPA seems to be a satisfactory alternative to inhibits the effects of Zika virus infection in mammalian cells.

Neurological Complications of Congenital Zika Virus Infection

2019 ◽  
Vol 91 ◽  
pp. 3-10 ◽  
Author(s):  
Vinícius de Melo Marques ◽  
Camilla Sousa Santos ◽  
Isabella Godinho Santiago ◽  
Solomar Martins Marques ◽  
Maria das Graças Nunes Brasil ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Neurological Complications ◽  
Zika Virus Infection

scholarly journals JMX0207, a Niclosamide Derivative with Improved Pharmacokinetics, Suppresses Zika Virus Infection Both In Vitro and In Vivo

2020 ◽  
Vol 6 (10) ◽  
pp. 2616-2628 ◽  
Author(s):  
Zhong Li ◽  
Jimin Xu ◽  
Yuekun Lang ◽  
Xiaoyu Fan ◽  
Lili Kuo ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Zika Virus Infection

Seroprevalence of Zika virus in selected regions in Kenya

2019 ◽  
Vol 113 (12) ◽  
pp. 735-739 ◽  
Author(s):  
Bramuel Kisuya ◽  
Moses M Masika ◽  
Esto Bahizire ◽  
Julius O Oyugi
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Healthy Adult ◽  
Neurological Complications ◽  
Previous Exposure ◽  
Limited Data ◽  
Zika Virus Infection ◽  
Human Sera ◽  
Study Population ◽  
The University

Abstract Background The Zika virus pandemic in South America in 2015–2016 and the association of Zika virus infection with neurological complications such as microcephaly in newborns distressed the global community. There is limited data on the prevalence of Zika virus in Kenya despite evidence of its circulation in East Africa. This study aimed at assessing the seroprevalence of Zika virus in selected areas in Kenya. Methods Healthy adult human sera originally collected from Nairobi, Eldoret and Kisumu from 2009 to 2014 and archived at the University of Nairobi laboratories were examined for Zika virus antibodies. An IgG-based ELISA was used to screen 577 sera. Any serum tested positive by ELISA was confirmed for Zika virus infection by plaque reduction neutralization test (PRNT). Results The seroprevalence of Zika virus in the study population was about 0.2 % (1/577) as confirmed by PRNT. Additionally, three sera that were false positive by ELISA for Zika virus were confirmed as positive for dengue virus by PRNT. Conclusion There was evidence of low previous exposure to Zika virus in the study population. Of the three regions in Kenya where sera for this study were obtained, only Kisumu County had one case of previous exposure to Zika virus.

scholarly journals Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen

2016 ◽  
Vol 22 (10) ◽  
pp. 1101-1107 ◽  
Author(s):  
Miao Xu ◽  
Emily M Lee ◽  
Zhexing Wen ◽  
Yichen Cheng ◽  
Wei-Kai Huang ◽  
...  
Keyword(s):  
Cell Death ◽  
Virus Infection ◽  
Small Molecule ◽  
Zika Virus ◽  
Small Molecule Inhibitors ◽  
Drug Repurposing ◽  
Neural Cell ◽  
Zika Virus Infection

scholarly journals MAIT cells are activated in acute Dengue virus infection and after in vitro Zika virus infection

2018 ◽  
Vol 12 (1) ◽  
pp. e0006154 ◽  
Author(s):  
Dominic Paquin-Proulx ◽  
Vivian I. Avelino-Silva ◽  
Bianca A. N. Santos ◽  
Nathália Silveira Barsotti ◽  
Fabiana Siroma ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Zika Virus ◽  
Dengue Virus Infection ◽  
Mait Cells ◽  
Zika Virus Infection

Beneficial in vitro effects of the passion fruit seed extract against placental Zika virus infection

Placenta ◽  
2021 ◽  
Vol 112 ◽  
pp. e42
Author(s):  
Eloiza Tanabe ◽  
Jaqueline Santos ◽  
Keyla Pires ◽  
Monique Nova ◽  
Karen Borbely ◽  
...  
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
Seed Extract ◽  
Passion Fruit ◽  
Zika Virus Infection ◽  
In Vitro Effects

scholarly journals Autopsy and Postmortem Studies Are Concordant: Pathology of Zika Virus Infection Is Neurotropic in Fetuses and Infants With Microcephaly Following Transplacental Transmission

2016 ◽  
Vol 141 (1) ◽  
pp. 68-72 ◽  
Author(s):  
David A. Schwartz
Keyword(s):  
Virus Infection ◽  
Brain Damage ◽  
Vertical Transmission ◽  
Zika Virus ◽  
In Vivo Studies ◽  
Cytopathic Effects ◽  
Zika Virus Infection ◽  
Postmortem Studies

Context.—Pathology studies have been important in concluding that Zika virus infection occurring in pregnant women can result in vertical transmission of the agent from mother to fetus. Fetal and infant autopsies have provided crucial direct evidence that Zika virus can infect an unborn child, resulting in microcephaly, other malformations, and, in some cases, death. Objective.—To better understand the etiologic role and mechanism(s) of Zika virus in causing birth defects such as microcephaly, this communication analyzes the spectrum of clinical and autopsy studies reported from fetuses and infants who developed intrauterine Zika virus infection, and compares these findings with experimental data related to Zika virus infection. Design.—Retrospective analysis of reported clinical, autopsy, pathology, and related postmortem studies from 9 fetuses and infants with intrauterine Zika virus infection and microcephaly. Results.—All fetuses and infants examined demonstrated an overlapping spectrum of gross and microscopic neuropathologic abnormalities. Direct cytopathic effects of infection by the Zika virus were confined to the brain; in cases where other organs were evaluated, no direct viral effects were identified. Conclusions.—There is concordance of the spectrum of brain damage, reinforcing previous data indicating that the Zika virus has a strong predilection for cells of the fetal central nervous system following vertical transmission. The occurrence of additional congenital abnormalities suggests that intrauterine brain damage from Zika virus interferes with normal fetal development, resulting in fetal akinesia. Experimental in vitro and in vivo studies of Zika virus infection corroborate the human autopsy findings of neural specificity.

In vitro study of Zika virus infection in boar semen

2017 ◽  
Vol 162 (10) ◽  
pp. 3209-3213 ◽  
Author(s):  
Natthanej Luplertlop ◽  
San Suwanmanee ◽  
Sumate Ampawong ◽  
Sompong Vongpunsawad ◽  
Yong Poovorawan
Keyword(s):  
Virus Infection ◽  
Zika Virus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Zika Virus Infection ◽  
Boar Semen
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