scholarly journals Preventieve diergeneeskunde bij het konijn

2021 ◽  
Vol 90 (5) ◽  
pp. 252-255
Author(s):  
K. Hermans
Keyword(s):  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease

In dit artikel wordt een overzicht gegeven van belangrijke maatregelen die kunnen worden genomen in de preventie van ziekten bij konijnen. Op kop staat de jaarlijkse vaccinatie tegen myxomatose en “rabbit hemorrhagic disease” (RHD), een handeling die elke dierenarts zou moeten aanraden aan konijneneigenaars. Daarnaast is het verstrekken van correcte voeding ter preventie van onder andere tand- en darmproblemen welbekend. Verder worden een aantal huisvestingstips gegeven die vaak voorkomende gezondheidsproblemen bij konijnen kunnen helpen voorkomen. Obesitas, pododermatitis en urinewegproblemen zijn er enkele van. Minder aangeraden door dierenartsen, doch zeker niet onbelangrijk, is de preventie van uteriene tumoren bij voedsters door middel van electieve chirurgische wegname van ovaria en uterus, bij voorkeur vóór de leeftijd van twee jaar. Een gedegen kennis van de konijnendiergeneeskunde bij de dierenarts en een goede communicatie met konijneneigenaars over hoe ziekte bij hun dieren te voorkomen, kunnen bijdragen tot een lang en gezond leven van gezelschapskonijnen.

scholarly journals A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

2021 ◽  
Vol 9 (5) ◽  
pp. 972
Author(s):  
Joana Abrantes ◽  
Ana M. Lopes
Keyword(s):  
Disease Virus ◽  
Diagnostic Tests ◽  
High Throughput Sequencing ◽  
Current Knowledge ◽  
European Rabbit ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
Detection And Diagnosis

Since the early 1980s, the European rabbit (Oryctolagus cuniculus) has been threatened by the rabbit hemorrhagic disease (RHD). The disease is caused by a lagovirus of the family Caliciviridae, the rabbit hemorrhagic disease virus (RHDV). The need for detection, identification and further characterization of RHDV led to the development of several diagnostic tests. Owing to the lack of an appropriate cell culture system for in vitro propagation of the virus, much of the methods involved in these tests contributed to our current knowledge on RHD and RHDV and to the development of vaccines to contain the disease. Here, we provide a comprehensive review of the RHDV diagnostic tests used since the first RHD outbreak and that include molecular, histological and serological techniques, ranging from simpler tests initially used, such as the hemagglutination test, to the more recent and sophisticated high-throughput sequencing, along with an overview of their potential and their limitations.

A new variant of rabbit hemorrhagic disease virus G2-like strain isolated in China

2016 ◽  
Vol 215 ◽  
pp. 20-24 ◽  
Author(s):  
Bo Hu ◽  
Zhiyu Fan ◽  
Fang Wang ◽  
Yanhua Song ◽  
Houjun Wei ◽  
...  
Keyword(s):  
Disease Virus ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
New Variant

An Outbreak of Rabbit Hemorrhagic Disease in British Columbia, Canada

2021 ◽  
Vol 57 (4) ◽  
Author(s):  
Chelsea G. Himsworth ◽  
Doris Leung ◽  
Kaylee Byers ◽  
Tomy Joseph ◽  
Emilia Gordon ◽  
...  
Keyword(s):  
British Columbia ◽  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease

scholarly journals Identification and characterization of the virus causing rabbit hemorrhagic disease.

1990 ◽  
Vol 64 (7) ◽  
pp. 3331-3336 ◽  
Author(s):  
V F Ohlinger ◽  
B Haas ◽  
G Meyers ◽  
F Weiland ◽  
H J Thiel
Keyword(s):  
Hemorrhagic Disease ◽  
Rabbit Hemorrhagic Disease ◽  
Identification And Characterization

scholarly journals Minor structural protein VP2 in rabbit hemorrhagic disease virus downregulates the expression of the viral capsid protein VP60

2009 ◽  
Vol 90 (12) ◽  
pp. 2952-2955 ◽  
Author(s):  
Liu Chen ◽  
Guangqing Liu ◽  
Zheng Ni ◽  
Bin Yu ◽  
Tao Yun ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Disease Virus ◽  
Luciferase Assay ◽  
Rabbit Hemorrhagic Disease Virus ◽  
Hemorrhagic Disease ◽  
Mrna Levels ◽  
Structural Protein ◽  
Rabbit Hemorrhagic Disease ◽  
Minor Structural Protein ◽  
Capsid Protein Vp60

Rabbit hemorrhagic disease virus (RHDV) has two structural proteins: the major capsid protein VP60 and the minor capsid protein VP2. VP2 is speculated to play an important role in the virus life cycle. To investigate the effect of VP2 on VP60 expression, three types of experiment (baculovirus–insect cell system, mammalian–luciferase assay system and in vitro coupled transcription/translation system) were used to express VP60 alone or co-expressed with VP2. Both forms of VP60 were able to form virus-like particles in insect cells. Western blot analysis and dual-luciferase assays demonstrated that the presence of VP2 results in downregulation of the expression of VP60 in vivo. Real-time RT-PCR of mRNA levels showed that downregulation of VP60 occurs at the transcriptional level. The ability of the viral minor structural protein VP2 to regulate capsid protein levels may contribute to effective virus infection.

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