scholarly journals Genetic characterization of the domestic pig (Sus scrofa domestica) in Cerete-Colombia, using microsatellite markers

2014 ◽  
pp. 4150-4157
Author(s):  
Iván Meléndez G ◽  
Enrique Pardo P ◽  
Teodora Cavadia M
Keyword(s):  
Sus Scrofa ◽  
Polyacrylamide Gel Electrophoresis ◽  
Proteinase K ◽  
Domestic Pig ◽  
Expected Heterozygosity ◽  
Hair Samples ◽  
Pcr Products ◽  
High Degree ◽  
Sus Scrofa Domestica

ABSTRACTObjective. The purpose of this study was to characterize a population of domestic pig (Sus scrofa domestica) in Cereté, Córdoba, using 20 microsatellite; calculate heterozygosity per locus and average heterozygosity. Materials and methods. Hair samples were collected from 62 specimens. DNA was extracted by proteinase K digestion and phenol-chloroform purification. Information from 20 microsatellites was selected out of those recommended for swine biodiversity studies. PCR products were separated by a vertical polyacrylamide gel electrophoresis. The bands were visualized by staining with silver nitrate. Results. All microsatellites used were polymorphic. Between 3 (SW1067) and 15 (IFNG) alleles were detected with an average number of 6.7 and a total de 134 alleles. The average expected and observed heterozygosities were 0.5278 and 0.5479, respectively. PIC values ranged between 0.1999 and 0.8300 for loci SW1067 and SW911, respectively. Conclusions. Levels of observed and expected heterozygosity found in the present study indicate that the domestic pig (Sus scrofa domestica) in Córdoba Cereté show high degree of genetic variability

Isolation and Preliminary Characterization of a Bacteriocin Produced by Lactobacillus plantarum N014 Isolated from Nham, a Traditional Thai Fermented Pork

2006 ◽  
Vol 69 (8) ◽  
pp. 1937-1943 ◽  
Author(s):  
PONGSAK RATTANACHAIKUNSOPON ◽  
PARICHAT PHUMKHACHORN
Keyword(s):  
Lactobacillus Plantarum ◽  
Proteolytic Enzymes ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Proteinase K ◽  
Exponential Growth Phase ◽  
Plasmid Isolation ◽  
Inhibitory Spectrum ◽  
Lipase A

Lactobacillus plantarum N014 was isolated from nham, a traditional Thai fermented pork, and exhibited antimicrobial activity against Listeria monocytogenes. Its bacteriocin had a broad inhibitory spectrum toward both gram-positive and gram-negative bacteria. The bacteriocin activity was sensitive to all proteolytic enzymes used in this study, including papain, pepsin, pronase E, proteinase K, and trypsin, but was resistant to the other enzymes, such as α-amylase, lipase A, and lysozyme. Furthermore, activity was stable over various heat treatments and pH values. The bacteriocin exerted a bacteriolytic mode of action. It was produced during the exponential growth phase and reached its highest level as producer cells entered the stationary phase. Adsorption of the bacteriocin onto producer cells was pH-dependent. No bacteriocin adsorption was detected at pH 1 to 3, whereas 100% bacteriocin adsorption was found at pH 7. Plasmid isolation revealed that L. plantarum N014 contained no plasmids. From Tricine–sodium dodecyl sulfate–polyacrylamide gel electrophoresis and growth inhibition testing against L. monocytogenes, the estimated molecular mass of L. plantarum N014 bacteriocin was 8 kDa.

scholarly journals Nine new cases of reciprocal translocation in the domestic pig (Sus scrofa domestica L.)

1998 ◽  
Vol 89 (2) ◽  
pp. 136-142 ◽  
Author(s):  
A. Ducos ◽  
H. M. Berland ◽  
A. Pinton ◽  
E. Guillemot ◽  
A. Seguela ◽  
...  
Keyword(s):  
Reciprocal Translocation ◽  
Sus Scrofa ◽  
Domestic Pig ◽  
Sus Scrofa Domestica

The cytogenetic map of the domestic pig (Sus scrofa domestica)

1997 ◽  
Vol 8 (8) ◽  
pp. 592-607 ◽  
Author(s):  
Martine Yerle ◽  
Yvette Lahbib-Mansais ◽  
Philippe Pinton ◽  
Annie Robic ◽  
André Goureau ◽  
...  
Keyword(s):  
Sus Scrofa ◽  
Cytogenetic Map ◽  
Domestic Pig ◽  
Sus Scrofa Domestica

Vagal projections to the pylorus in the domestic pig (Sus scrofa domestica)

2012 ◽  
Vol 171 (1-2) ◽  
pp. 21-27 ◽  
Author(s):  
M. Zalecki ◽  
P. Podlasz ◽  
Z. Pidsudko ◽  
J. Wojtkiewicz ◽  
J. Kaleczyc
Keyword(s):  
Sus Scrofa ◽  
Domestic Pig ◽  
Sus Scrofa Domestica

scholarly journals Caracterización molecular de Heliconia sp. Utilizando la técnica RAPD.

2019 ◽  
pp. 230-233
Keyword(s):  
Molecular Marker ◽  
Molecular Characterization ◽  
Genomic Dna ◽  
Average Quality ◽  
Expected Heterozygosity ◽  
Apartado Postal ◽  
Pcr Products ◽  
Version 2.0

Caracterización molecular de Heliconia sp. Utilizando la técnica RAPD. Molecular characterization of Heliconia sp. using RAPD technique. Freddy Gutierrez, Roberson Ramirez, Pedro Adrianzén, Marianela Cobos, Sergio Pinedo, Sixto Imán, Juan Castro Centro de Investigaciones de Recursos Naturales de la Amazonia (CIRNA), 496S. Universidad Nacional de la Amazonia Peruana-UNAP. Apartado postal 496S Facultad de Ciencias Biológicas. Instituto Nacional de Innovación Agraria (INIA), C.P. 609. DOI: https://doi.org/10.33017/RevECIPeru2011.0051/ RESUMEN Este trabajo se realizó entre los meses de Julio a Setiembre del 2010, con el objetivo de caracterizar molecularmente a la especie Heliconia sp. Utilizando el marcador molecular RAPD. Para esto, se realizó la purificación del ADN genómico de la especie en estudio obteniéndose buenos resultados en cuanto a la calidad y la cantidad del ADN purificado, el cual fue verificado con los métodos electroforético y espectrofotométrico, la cantidad de ADN obtenido en promedio fue de 92.8 ng/ μl, asimismo el ratio de calidad promedio fue de 2.2. Para la amplificación de los productos de la PCR se empleó el iniciador MT3R, obteniéndose mucho polimorfismo. Los productos de la PCR tuvieron tamaños de 750 pb hasta 2050 pb. En cuanto a la caracterización molecular, se realizó un dendograma (UPGMA) utilizando el programa estadístico NTSIS versión 2.0. Asimismo se obtuvo la formación de 2 grupos (clusters) y 2 fugas donde se observó la aparición de “aparentes duplicados” en algunos de ellos, es decir, que comparten el mismo patrón genético con un 100% de similitud. Además el valor de la heterocigosidad esperada fue de 0.14, valor relativamente bajo, posiblemente al tipo de reproducción que posee este género, el cual es la autogamica. Descriptores: ADN genómico, Heliconia sp, electroforético, espectrofotométrico. ABSTRACT This work was carried out between the months of July to September 2010 with the objective of molecular characterization of the species heliconia sp using the RAPD molecular marker. For this, we performed the purification of genomic DNA, of the species under study with good results in terms of quality and quantity of purified DNA, which was verified by electrophoretic and spectrophometric methods, the amount of DNA obtained was on average 92.8 ng/ l, also the ratio of average quality was 2.2. For amplification of PCR products was used MT3R initiator, obtaining much polymorphism. PCR products had sizes of 750 bp to 2050 bp. as for the molecular characterization, we performed a dendrogram (UPGMA) using the statiscal sofwar version 2.0 NTSIS. It also obtained the formation of 2 groups (clusters) and 2 leaks where we the appearance  of “apparent duplicates” in some of them, ie they share the same genetic patern with 100% similarity. Also, the value of expected heterozygosity was 0.14, relatively low value, possibly the type of reproduction that has this kind, which is the autogamous. Keywords: genomic DNA, Heliconia sp, electrophoretic, spectrophometric.

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