scholarly journals Sequence variability in p27 gene of Citrus Tristeza Virus (CTV) revealed by SSCP analysis

1999 ◽  
Vol 2 (1) ◽  
Author(s):  
Liliana Semorile ◽  
Selma Gago-Zachert ◽  
Norma Costa ◽  
Oscar Grau
Keyword(s):  
Citrus Tristeza Virus ◽  
Sscp Analysis ◽  
Sequence Variability ◽  
P27 Gene ◽  
Tristeza Virus ◽  
Citrus Tristeza

Sscp analysis of variations in haplotypes of citrus tristeza virus isolated from yuzu (Citrus junos) in geographically separate regions of Korea

2006 ◽  
Vol 49 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Dae Hyun Kim ◽  
Hye Kyung Shim ◽  
Jae Wook Hyeon ◽  
Hyeog Mo Kwon ◽  
Kwang Sik Kim ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Sscp Analysis ◽  
Citrus Junos ◽  
Tristeza Virus ◽  
Citrus Tristeza

SSCP analysis of Citrus tristeza virus protectives isolates in Pêra sweet orange clones under northern Paraná state, Brazil conditions

2011 ◽  
Vol 32 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Walter Magri Temporal ◽  
Maria Júlia Corazza ◽  
Carlos Alexandre Zanutto ◽  
William Mário de Carvalho Nunes ◽  
Gerd Walter Müller
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Sscp Analysis ◽  
Tristeza Virus ◽  
Citrus Tristeza

First report of citrus tristeza virus in commercial citrus orchards in Tunisia

2021 ◽  
Author(s):  
Asma Najar ◽  
Imen Hamdi ◽  
Souad Mahmoud ◽  
Lassaad Medhioub ◽  
Imed Jaouadi ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Citrus Orchards ◽  
Tristeza Virus ◽  
Citrus Tristeza

A model for the spread and control of citrus tristeza virus disease

1989 ◽  
Vol 16 (3) ◽  
pp. 315-320
Author(s):  
Ruth Marcus ◽  
Hovav Talpaz ◽  
Moshe Bar-Joseph
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
And Control ◽  
Tristeza Virus ◽  
Citrus Tristeza

Production of Antibodies to Citrus Tristeza Virus in Transgenic Citrus

1995 ◽  
Author(s):  
Richard Lee ◽  
Moshe Bar-Joseph ◽  
K.S. Derrick ◽  
Aliza Vardi ◽  
Roland Brlansky ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
Biological Activities ◽  
Cp Gene ◽  
Single Domain Antibodies ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) is the most important virus disease of citrus in the world. CTV causes death of trees on sour orange rootstock and/or stem pitting of scions regardless of rootstock which results in trees of low vigor, reduced yield with reduction in size and quality of fruit. The purpose of this project was to produce monoclonal antibodies (MABs) to CTV coat protein (CP), develop single domain antibodies (dAbs) or Fab fragments which neutralize the infection by binding to the virus, and to produce transformed plants which express the dAbs. The objectives of this research have been met and putative transgenic tobacco and citrus plants have been developed. These putative transgenic plants are presently undergoing evaluation to determine the level of dAbs expression and to determine their resistance to CTV. Additionally, the CTV genome has been sequenced and the CP gene of several biologically characterized CTV strains molecular characterized. This has indicated a correlation between CP sequence homology and biological activity, and the finding of DI RNAs associated with some CTV strains. Several MABs have been produced which enable broad spectrum identification of CTV strains while other MABs enable differentiation between mild and severe strains. The use of selected MAbs and determination of the CP gene sequence has enabled predictions of biological activities of unknown CTV isolates. The epitopes of two MABs, one reacting selectively with severe CTV strains and the other reacting with all strains, have been characterized at the molecular level.

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