Normal-phase thin-layer chromatography of some angiotensin converting enzyme (ACE) inhibitors and their metabolites

The separation and chromatographic behaviour of five ACE (angiotensin converting enzyme) inhibitors and their four active metabolites were investigated by normal-phase thin-layer chromatography on silica using several mono- and binary non-aqueous solvent systems. The linear relationship between the RM values and the composition of employed mobile phase was obtained. The hydrophobicity parameters 0M R and C0 were determined from the regression data of the plots, analogous to reversed-phase chromatography. The chromatographically obtained hydrophobicity parameters were correlated with the calculated log P values. The current results were correlated with the lipophilicity of the studied ACE inhibitors and their metabolites, previously estimated by reversed-phase chromatography.

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Normal‐phase versus reversed‐phase thin‐layer chromatography (TLC) to monitor oxidized phosphatidylcholines by TLC/mass spectrometry

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.8237 ◽

2018 ◽

Vol 33 (S1) ◽

pp. 60-65 ◽

Cited By ~ 3

Author(s):

Kathrin M. Engel ◽

Hans Griesinger ◽

Michael Schulz ◽

Jürgen Schiller

Keyword(s):

Mass Spectrometry ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Reversed Phase ◽

Normal Phase ◽

Phase Versus ◽

Layer Chromatography

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Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors

Journal of Chromatography A ◽

10.1016/j.chroma.2011.03.039 ◽

2011 ◽

Vol 1218 (20) ◽

pp. 3089-3094 ◽

Cited By ~ 32

Author(s):

Irena Vovk ◽

Gordana Popović ◽

Breda Simonovska ◽

Alen Albreht ◽

Danica Agbaba

Keyword(s):

Mass Spectrometry ◽

Angiotensin Converting Enzyme ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Enzyme Inhibitors ◽

Angiotensin Converting Enzyme Inhibitors ◽

Converting Enzyme ◽

Converting Enzyme Inhibitors ◽

Chromatography Mass Spectrometry ◽

Layer Chromatography

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Reversed-phase and normal-phase thin-layer chromatography and their application to the lipophilicity prediction of synthetic pyrethroids based on quantitative structure–retention relationships

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/1006.2018.31.2.1 ◽

2018 ◽

Vol 31 (2) ◽

pp. 99-104 ◽

Cited By ~ 1

Author(s):

Joanna Nowakowska ◽

Krzesimir Ciura ◽

Piotr Kawczak ◽

Bartosz Wielgomas ◽

Tomasz Bączek

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Reversed Phase ◽

Normal Phase ◽

Synthetic Pyrethroids ◽

Quantitative Structure ◽

Structure Retention ◽

Lipophilicity Prediction ◽

Layer Chromatography ◽

Quantitative Structure Retention Relationships

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Reversed-phase thin-layer chromatography of some angiotensin converting enzyme (ACE) inhibitors and their active metabolites

Journal of the Serbian Chemical Society ◽

10.2298/jsc0606621o ◽

2006 ◽

Vol 71 (6) ◽

pp. 621-628 ◽

Cited By ~ 10

Author(s):

Jadranka Odovic ◽

Biljana Stojimirovic ◽

Mirjana Aleksic ◽

Dusanka Milojkovic-Opsenica ◽

Zivoslav Tesic

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Ace Inhibitors ◽

Reversed Phase ◽

Log P ◽

Binary Solvent ◽

Active Metabolites ◽

Solvent Systems ◽

Layer Chromatography ◽

Lipophilicity Parameters

The chromatographic behaviour of some ACE inhibitors and their active metabolites was examined under conditions of reversed-phase thin-layer chromatography on RP-18 silica using water-methanol, water-ethanol and water-acetone as binary solvent systems. The relationship between the R M values and the concentration of organic modifier in the mobile phases was linear. It was found that an increase in the content of the organicmodifier in the employed solvent systems led to a decrease of the R M values, i.e., of the retention. Also, the more hydrophobic compounds had a longer retention. Based on regression analysis of the plots, the lipophilicity parameters R M 0 and c 0 were calculated. The chromatographically obtained lipophilicity parameters were correlated with the calculated log P values.

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