Abstract
Ciguatera fish poisoning is the most extensive and difficult to control of the seafood poisonings. Tofacilitate monitoring of fish toxicity, toxin profiles were investigated by an LC/MS/MS method using 14reference toxins on eight representative species of fish collected in four different areas of the Pacific. Snappers and groupers from Okinawa contained ciguatoxin-1B (CTX1B) and two deoxy congeners at variablebut species-specific ratios, while red snapper, Lutjanus bohar, from Minamitorishima, and amberjack, Seriola dumerili, from Hawaii, contained both CTX1B-type and CTX3C-type toxins. Spotted knifejaw, Oplegnathus punctatus, from Okinawan waters, contained mainly CTX4A and CTX4B, but the same species caught at Miyazaki was contaminated primarily with the CTX3C-type toxins. Otherwise, the toxin profiles were consistently species-specific in fish collected from various locations around Okinawa over 20 years. The LC/MS/MSand mouse bioassay results agreed well, indicating the LC/MS/MS method is a promising alternative to themouse bioassay. Pure CTX1B and CTX3C were prepared for use in future LC/MS/MS analysis.
From the cradle to the grave: Green turtle hatchlings (Chelonia mydas) preyed upon by two-spots red snappers (Lutjanus bohar)