Phenylurea cytokinins assayed for induction of shoot buds in the moss Funaria hygrometrica

Background: The erosion of mosses on the surface of Hue imperial citadel has caused a lot of harm and is currently an urgent problem to be solved. Objectives:1. Identifying some moss species growing on Hue relics. 2. Determining the cause of slipperiness and the living environment mosses. 3. Finding a solution to limit the intrusion of the mosses. Methods: The methods are: identifying scientific name by comparing patterns, identify mucus, pH measurement and evaluation of the effects of certain chemicals on the growth of mosses in the laboratory. Result: The moss species that develop popularly in Hue relics is Funaria hygrometrica, Bryum apiculatum, Trichostomum orthodontum, Fissidens lycopodioides. Funaria hygrometrica is the most popular moss. The cause of the slipperiness is mucus in moss cells released outside the moss after death. Mosses grow well in moist environments, clear and slightly acidic surface. After two days assessing the effect of citric acid 18%, 15%, 10% and 7% NaHCO3, 5%, 3% on the growth of moss, the mosses all died completely. Since then identified two types of chemicals have been found to completely dead moss, inexpensive, environmentally friendly and does not affect the surface of architectures are citric acid 10% solution and NaHCO3 3% solution. Keywords: The moss erosion, Funaria hygrometrica, mucus, citric acid, NaHCO3 Key words: The moss erosion, Funaria hygrometrica, mucus, citric acid, NaHCO 3

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Plant Regeneration from Excised Cotyledons of Mature Strawberry Achenes

HortScience ◽

10.21273/hortsci.25.5.569 ◽

1990 ◽

Vol 25 (5) ◽

pp. 569-571 ◽

Cited By ~ 5

Author(s):

A. Raymond Miller ◽

Craig K. Chandler

Keyword(s):

Acetic Acid ◽

Plant Regeneration ◽

Multiple Shoot ◽

Naphthaleneacetic Acid ◽

Developmental Studies ◽

Shoot Buds ◽

Cotyledon Explants ◽

Multiple Shoot Buds ◽

Rapid Regeneration ◽

Dichlorophenoxy Acetic Acid

A protocol was developed for excising and culturing cotyledon explants from mature achenes of strawberry (Fragaria × ananassa Duch.). Cotyledon explants formed callus with multiple shoot buds on agar-solidified Murashige and Skoog media containing several combinations of hormones (1 μm 2,4-D; 10 μm 2,4-D; 1 μm BA + 1 μm 2,4-D; 1 μm BA + 10 μm 2,4-D; 5 μm BA; 5 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μ m 2,4-D; 5 μ m BA + 5 μm NAA; 5 μ m BA + 15 μ m NAA). After three subcultures, only tissues maintained on the medium containing 5 μm BA + 5 μm NAA continued to form shoots. Tissues transferred to other media eventually died (1 μm 2,4-D; 1 μ m BA + 10 μ m 2,4-D; 5 μ m BA; 5 μ m BA + 1 μ m 2,4-D), became unorganized (1 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μm 2,4-D; 5 μm BA + 15 μm NAA), or formed roots (10 μm 2,4-D). Whole plantlets were produced by transferring callus with buds to medium lacking hormones. The rapid regeneration of clonal plantlets from cotyledon explants may be useful for reducing variability in future developmental studies. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D); and 1-naphthaleneacetic acid (NAA).

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