Osmium-Thiocarbohydrazide-Osmium versus Tannic Acid-Osmium Staining of Skeletal Muscle for Scanning Electron Microscopy and Correlative Microscopy

Ruthenium tetroxide(RuO4) was first used as a stain in histology by Ranvier in 1887. Its use in TEM as a fixative or stain was reported by Gaylarde et al. However,the preservation of the cellular ultrastructure was very poor after RuO4 fixation alone, because RuO4 is a far more vigorous oxidant than osmium tetroxide. Peltari et al reported that a preceeding glutaraldehyde fixation helped to stabilize the ultrastructure, although the penetration of RuO4 into the tissue was poor.In the present study, RuO4 has been found to be a useful fixative and staining reagent with the prerequisite of using RuO4 as a postfixative after prefixation with tannic acid (TA)-glutaraldehyde(GL) for thin-section TEM(TA-RuO4 method) or after preceeding osmication followed by TA mordanting for non-coating SEM(OsO4-TA-RuO4 method).TA-RuO4 method for thin-section TEM

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SCANNING ELECTRON MICROSCOPY OF SKELETAL MUSCLE FROM RAINBOW TROUT, TURKEY AND BEEF

Journal of Food Science ◽

10.1111/j.1365-2621.1971.tb15127.x ◽

1971 ◽

Vol 36 (4) ◽

pp. 552-559 ◽

Cited By ~ 30

Author(s):

D. R. SCHALLER ◽

W. D. POWRIE

Keyword(s):

Electron Microscopy ◽

Skeletal Muscle ◽

Scanning Electron Microscopy ◽

Rainbow Trout ◽

Scanning Electron

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Correlative electrochemical strain and scanning electron microscopy for local characterization of the solid state electrolyte Li1.3Al0.3Ti1.7(PO4)3

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.9.148 ◽

2018 ◽

Vol 9 ◽

pp. 1564-1572 ◽

Cited By ~ 15

Author(s):

Nino Schön ◽

Deniz Cihan Gunduz ◽

Shicheng Yu ◽

Hermann Tempel ◽

Roland Schierholz ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Solid State ◽

Electrochemical Impedance ◽

Secondary Phase ◽

Ion Conductivity ◽

Correlative Microscopy ◽

Li Ion ◽

Solid State Electrolytes ◽

Scanning Electron

Correlative microscopy has been used to investigate the relationship between Li-ion conductivity and the microstructure of lithium aluminum titanium phosphate (Li1.3Al0.3Ti1.7(PO4)3, LATP) with high spatial resolution. A key to improvement of solid state electrolytes such as LATP is a better understanding of interfacial and ion transport properties on relevant length scales in the nanometer to micrometer range. Using common techniques, such as electrochemical impedance spectroscopy, only global information can be obtained. In this work, we employ multiple microscopy techniques to gain local chemical and structural information paired with local insights into the Li-ion conductivity based on electrochemical strain microscopy (ESM). Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) have been applied at identical regions to identify microstructural components such as an AlPO4 secondary phase. We found significantly lower Li-ion mobility in the secondary phase areas as well as at grain boundaries. Additionally, various aspects of signal formation obtained from ESM for solid state electrolytes are discussed. We demonstrate that correlative microscopy is an adjuvant tool to gain local insights into interfacial properties of energy materials.

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The Effect of Tannic Acid on the Preservation of Tissue Culture Cells for Scanning Electron Microscopy

Journal of Electron Microscopy ◽

10.1093/oxfordjournals.jmicro.a050303 ◽

1981 ◽

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Tissue Culture ◽

Tannic Acid ◽

Culture Cells ◽

Tissue Culture Cells ◽

Scanning Electron

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Pathogenesis of Human Hair Defects

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010005007x ◽

1974 ◽

Vol 32 ◽

pp. 12-13

Author(s):

P.S. Porter ◽

T. Aoyagi ◽

R. Matta

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Human Hair ◽

Standard Techniques ◽

Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

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Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080614 ◽

1977 ◽

Vol 35 ◽

pp. 638-639

Author(s):

P.J. Dailey

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Salivary Glands ◽

Secretory Vesicles ◽

The Past ◽

Transmission Electron ◽

Means Of Transmission ◽

Gromphadorhina Portentosa ◽

Scanning Electron ◽

Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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