Temperature Dependence of the Direct Action of Ionizing Radiation on Beef Heart Lactate Dehydrogenase: Enzyme Activity, Substrate and Coenzyme Affinities

1972 ◽  
Vol 51 (1) ◽  
pp. 56 ◽  
Author(s):  
Donald J. Fluke
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Ionizing Radiation ◽  
Temperature Dependence ◽  
Direct Action ◽  
Beef Heart ◽  
Dehydrogenase Enzyme ◽  
Dehydrogenase Enzyme Activity

IN VITRO STEROIDOGENESIS IN NORMAL HUMAN OVARIAN TISSUE

1966 ◽  
Vol 52 (3) ◽  
pp. 455-464 ◽  
Author(s):  
Lakshmi Kumari ◽  
Joseph W. Goldzieher
Keyword(s):  
Enzyme Activity ◽  
Luteal Phase ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Dehydrogenase Enzyme ◽  
Normal Human ◽  
Dehydrogenase Enzyme Activity

ABSTRACT Eleven samples of normal human ovarian tissue from the various phases of the cycle were incubated with 7-3H progesterone and 4-14C-5-pregnenolone. Isolation, identification and quantitation of the metabolites showed clearly that pregnenolone is a far better substrate than progesterone for the production of C19 steroids. Under the conditions of these incubations, 3β-ol dehydrogenase enzyme activity converted the majority of the metabolites into androstenedione. The accumulation of radioactive androstenedione at the end of the incubation exceeded that of testosterone except in the later part of the luteal phase.

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