IN VITRO STEROIDOGENESIS IN NORMAL HUMAN OVARIAN TISSUE

1966 ◽  
Vol 52 (3) ◽  
pp. 455-464 ◽  
Author(s):  
Lakshmi Kumari ◽  
Joseph W. Goldzieher
Keyword(s):  
Enzyme Activity ◽  
Luteal Phase ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Dehydrogenase Enzyme ◽  
Normal Human ◽  
Dehydrogenase Enzyme Activity

ABSTRACT Eleven samples of normal human ovarian tissue from the various phases of the cycle were incubated with 7-3H progesterone and 4-14C-5-pregnenolone. Isolation, identification and quantitation of the metabolites showed clearly that pregnenolone is a far better substrate than progesterone for the production of C19 steroids. Under the conditions of these incubations, 3β-ol dehydrogenase enzyme activity converted the majority of the metabolites into androstenedione. The accumulation of radioactive androstenedione at the end of the incubation exceeded that of testosterone except in the later part of the luteal phase.

METABOLISM OF [4-14C] PROGESTERONE BY COMPONENTS OF NORMAL HUMAN OVARIES

1970 ◽  
Vol 65 (4) ◽  
pp. 707-711 ◽  
Author(s):  
Leonard R. Axelrod ◽  
Joseph W. Goldzieher
Keyword(s):  
Ovarian Tissue ◽  
Regulatory Mechanisms ◽  
Human Ovarian Tissue ◽  
Normal Human ◽  
Human Ovaries

ABSTRACT Follicular, luteal and whole human ovarian tissue were incubated in vitro with [4-14C] progesterone and cofactors. Each of these tissues showed a potential for carrying out a large variety of enzymatic transformations such as 6β and 17α hydroxylations and 20α and 20β reductions, suggesting that all these »anatomical compartments« have the capability of participating in the biosynthesis of a large number of normal intermediates; and their »normal« or anomalous production would be governed by the regulatory mechanisms peripheral to the ovary which themselves may be altered pathologically from the normal. The presence of 19-nor-C19 compounds is unexplained but substantiated by previous work.

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model

Slush nitrogen vitrification of human ovarian tissue does not alter gene expression and improves follicle health and progression in long-term in vitro culture

2018 ◽  
Vol 110 (7) ◽  
pp. 1356-1366 ◽  
Author(s):  
Vincenza Barbato ◽  
Roberto Gualtieri ◽  
Teresa Capriglione ◽  
Maria Michela Pallotta ◽  
Sabrina Braun ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Culture ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue

BIOSYNTHESIS OF TWO STEROID GLYCOLS FROM 17α-HYDROXYPROGESTERONE BY SURVIVING HUMAN OVARIAN SLICES

1960 ◽  
Vol 38 (1) ◽  
pp. 1167-1172
Author(s):  
Thomas Sandor ◽  
André Lanthier
Keyword(s):  
Sulphuric Acid ◽  
Ovarian Tissue ◽  
Oxygen Atmosphere ◽  
Chromatographic Mobility ◽  
Tissue Slices ◽  
Medium Ph ◽  
Human Ovarian Tissue ◽  
Mobility Studies ◽  
Normal Human ◽  
Phosphate Medium

Surviving human ovarian slices were incubated with 17α-hydroxyprogesterone in a Krebs–Ringer phosphate medium (pH 7.4), at 37 °C under an oxygen atmosphere. The substrate was partially transformed to two steroid glycols, designated as X1and X2. Chromatographic mobility studies, derivatization, and spectra in 95% ethanol and in concentrated sulphuric acid suggested that X1might be identical with Δ4-pregnene-17α,20α-diol-3-one, and X2with Δ4-pregnene-17α,20β-diol-3-one. Synthetic Δ4-pregnene-17α,20β-diol-3-one was prepared from 17α-hydroxyprogesterone. It was found identical with X2by the above-mentioned criteria.In addition to normal human ovarian tissue, slices of human Stein–Leventhal type ovaries were also incubated with the precursor and the transformation rates to X1and X2calculated for both types of tissues.Experiments were performed using as substrate Δ5-pregnene-3β-ol-20-one and progesterone in order to study the reaction with biosynthetic 17α-hydroxyprogesterone. The transformation to X1and X2seemed to follow the same course as with synthetic 17α-hydroxyprogesterone.

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