DNA Double-Strand Breaks Induced by High-Energy Neon and Iron Ions in Human Fibroblasts. II. Probing Individual NotI Fragments by Hybridization

1994 ◽  
Vol 139 (2) ◽  
pp. 142 ◽  
Author(s):  
Markus Löbrich ◽  
Björn Rydberg ◽  
Priscilla K. Cooper ◽  
Markus Lobrich ◽  
Bjorn Rydberg
Keyword(s):  
Human Fibroblasts ◽  
High Energy ◽  
Double Strand Breaks ◽  
Iron Ions ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

scholarly journals Homologous Recombination Contributes to the Repair of DNA Double-Strand Breaks Induced by High-Energy Iron Ions

2010 ◽  
Vol 173 (1) ◽  
pp. 27 ◽  
Author(s):  
Faria Zafar ◽  
Sara B. Seidler ◽  
Amy Kronenberg ◽  
David Schild ◽  
Claudia Wiese
Keyword(s):  
Homologous Recombination ◽  
High Energy ◽  
Double Strand Breaks ◽  
Iron Ions ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

scholarly journals Posttranslational Modifications of p53 in Replicative Senescence Overlapping but Distinct from Those Induced by DNA Damage

2000 ◽  
Vol 20 (8) ◽  
pp. 2803-2808 ◽  
Author(s):  
Katherine Webley ◽  
Jane A. Bond ◽  
Christopher J. Jones ◽  
Jeremy P. Blaydes ◽  
Ashley Craig ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
Replicative Senescence ◽  
Human Fibroblasts ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Direct Role ◽  
Strand Breaks ◽  
Serine 392 ◽  
Regulatory Sites ◽  
Telomere Erosion

ABSTRACT Replicative senescence in human fibroblasts is absolutely dependent on the function of the phosphoprotein p53 and correlates with activation of p53-dependent transcription. However, no evidence for posttranslational modification of p53 in senescence has been presented, raising the possibility that changes in transcriptional activity result from upregulation of a coactivator. Using a series of antibodies with phosphorylation-sensitive epitopes, we now show that senescence is associated with major changes at putative regulatory sites in the N and C termini of p53 consistent with increased phosphorylation at serine-15, threonine-18, and serine-376 and decreased phosphorylation at serine-392. Ionizing and UV radiation generated overlapping but distinct profiles of response, with increased serine-15 phosphorylation being the only common change. These results support a direct role for p53 in signaling replicative senescence and are consistent with the generation by telomere erosion of a signal which shares some but not all of the features of DNA double-strand breaks.

A microbeam study of DNA double-strand breaks in bystander primary human fibroblasts

2006 ◽  
Vol 122 (1-4) ◽  
pp. 256-259 ◽  
Author(s):  
L. B. Smilenov ◽  
E. J. Hall ◽  
W. M. Bonner ◽  
O. A. Sedelnikova
Keyword(s):  
Human Fibroblasts ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

255Residual DNA double-strand breaks and cellular radiosensitivity of 12 normal human fibroblasts

1996 ◽  
Vol 40 ◽  
pp. S67
Author(s):  
Ingo Brammer ◽  
Ekkehard Dikomey ◽  
Jørgen Johansen ◽  
Søren M. Bentzen ◽  
Jens Overgaard
Keyword(s):  
Human Fibroblasts ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Cellular Radiosensitivity ◽  
Strand Breaks ◽  
Normal Human
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