scholarly journals Isolation and Characterization of Microsatellite Loci of Clarias macrocephalus and Their Application to Genetic Diversity Study

1999 ◽  
Vol 65 (4) ◽  
pp. 520-526 ◽  
Author(s):  
Uthairat Na-Nakorn ◽  
Nobuhiko Taniguchi ◽  
Estu Nugroho ◽  
Shingo Seki ◽  
Wongpathom Kamonrat
HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 2028-2030 ◽  
Author(s):  
Xue-qin Wang ◽  
Yuan Huang ◽  
Chun-lin Long

Rhododendron decorum is a common species in southwest China and northeast Myanmar, in which the flowers have been eaten as a favorite vegetable. We isolated and characterized 24 microsatellite primer pairs from this species. The number of alleles ranged from two to seven. The observed and expected heterozygosities (HO and HE) were 0.3830 to 0.7855 and 0 to 0.7917, respectively. Eleven loci were significantly deviated from Hardy-Weinberg equilibrium as a result of the heterozygote deficiency. Cross-species amplification in another eight Rhododendron species showed their potential use for evolutionary and conservation studied in this genus. These markers will be useful to reveal the genetic population structure and genetic diversity of R. decorum.


HortScience ◽  
2008 ◽  
Vol 43 (6) ◽  
pp. 1925-1926 ◽  
Author(s):  
Kuaifei Xia ◽  
Xiulin Ye ◽  
Mingyong Zhang

Microsatellite-enhanced genomic library of Cymbidium sinense was constructed using repeat-enrichment method with biotin-labeled oligos and streptavidin magnetic beads. Twenty-five microsatellite loci were isolated from a microsatellite-enhanced genomic library of Cymbidium sinense. Nine of these loci displayed genetic diversity that ranged from one to 15 alleles per locus, and the observed heterozygosity of six polymorphic loci ranged from 0.18 to 0.90 with an average of 0.47 (0.29 sd) in a sample of 30 individuals. Eight of these loci were successfully amplified in at least one of the following species: Paphiopedilum hissutissimum, P. wardii, P. armeniacum, or P. micranthum. The study provides a base for molecular breeding and assessment of germplasm resources of the Cymbidium sinense and the results suggest that the microsatellite loci developed from C. sinense may have a broad applicability within the Orchidaceae family.


2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  

2014 ◽  
Vol 6 (2) ◽  
pp. 297-299 ◽  
Author(s):  
YingBiao Zhi ◽  
Ling Ding ◽  
Yupeng Wang ◽  
Longfei Hu ◽  
Yupeng Hua ◽  
...  

2008 ◽  
Vol 8 (4) ◽  
pp. 854-856 ◽  
Author(s):  
RENNAN G. MOREIRA ◽  
ROSS A. MCCAULEY ◽  
AUREA C. CORTÉS-PALOMEC ◽  
M. BERNADETE LOVATO ◽  
G. WILSON FERNANDES ◽  
...  

2014 ◽  
Vol 6 (3) ◽  
pp. 711-713
Author(s):  
Ping Qiu ◽  
Xuefen Yang ◽  
Kai-Jian Wei ◽  
Zhihong Ma ◽  
Ruibin Yang

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