scholarly journals Biodegradation of several fibers submerged in deep sea water and isolation of biodegradable plastic degrading bacteria from deep ocean water

2009 ◽  
Vol 75 (6) ◽  
pp. 1011-1018 ◽  
Author(s):  
TAKAYOSHI SEKIGUCHI ◽  
AKIRA EBISUI ◽  
KOJI NOMURA ◽  
TOSHIHIRO WATANABE ◽  
MAKIKO ENOKI ◽  
...  
Keyword(s):  
Deep Sea ◽  
Sea Water ◽  
Deep Ocean ◽  
Biodegradable Plastic ◽  
Ocean Water ◽  
Deep Sea Water ◽  
Deep Ocean Water ◽  
Degrading Bacteria

Alcanivorax marinus sp. nov., isolated from deep-sea water

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4428-4432 ◽  
Author(s):  
Qiliang Lai ◽  
Jianning Wang ◽  
Li Gu ◽  
Tianling Zheng ◽  
Zongze Shao
Keyword(s):  
16S Rrna ◽  
Deep Sea ◽  
Type Species ◽  
Sea Water ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Content Type ◽  
Deep Sea Water ◽  
Link Type ◽  
Degrading Bacteria

A taxonomic study was carried out on strain R8-12T, which was isolated from deep-sea water of the Indian Ocean during the screening of oil-degrading bacteria. The isolate was Gram-stain-negative, oxidase and catalase-positive. Growth was observed at salinities from 0.5 to 15 % (optimum 3 %), at pH from 6–10 (optimum 7–8) and at temperatures from 10 to 42 °C (optimum 28 °C). On the basis of 16S rRNA gene sequence similarity, strain R8-12T was shown to belong to the genus Alcanivorax and to be related to Alcanivorax venustensis DSM 13974T (97.2 %), A. dieselolei B-5T (95.0 %), A. balearicus MACL04T (94.6 %), A. hongdengensis A-11-3T (94.3 %), A. jadensis T9T (93.8 %), A. borkumensis SK2T (93.7 %) and A. pacificus W11-5T (93.7 %). The gyrB sequence similarities between R8-12T and other species of the genus Alcanivorax ranged from 77.9 % to 86.9 %. The major fatty acids were C16 : 0 (31.8 %), C18 : 1ω7c (20.3 %), C19 : 0ω8c cyclo (15.8 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (8.9 %). The polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two aminolipids (AL1–AL2) and two phospholipids (PL1–PL2). Three alkane hydroxylase (alkB) genes were identified in the genome. The G+C content of the chromosomal DNA was 66.1 mol%. DNA–DNA hybridization showed that strain R8-12T and A. venustensis DSM 13974T had a DNA–DNA relatedness of 63±3 %. According to its phenotypic features and fatty acid composition as well as the 16S rRNA and gyrB gene sequences, the novel strain represents a member of the genus Alcanivorax , but could be easily distinguished from all other known species of the genus Alcanivorax described to date. The name Alcanivorax marinus sp. nov. is proposed, with the type strain R8-12T ( = MCCC 1A00382T = LMG 24621T = CCTCC AB 208234T).

Draft genome of bagasse-degrading bacteria Bacillus aryabhattai GZ03 from deep sea water

2015 ◽  
Vol 19 ◽  
pp. 13-14 ◽  
Author(s):  
Jian Wen ◽  
Chong Ren ◽  
Nan Huang ◽  
Yang Liu ◽  
Runying Zeng
Keyword(s):  
Deep Sea ◽  
Sea Water ◽  
Draft Genome ◽  
Deep Sea Water ◽  
Bacillus Aryabhattai ◽  
Degrading Bacteria

scholarly journals Harnessing the power of eDNA metabarcoding for the detection of deep-sea fishes

2020 ◽  
Author(s):  
Beverly McClenaghan ◽  
Nicole Fahner ◽  
David Cote ◽  
Julek Chawarski ◽  
Avery McCarthy ◽  
...  
Keyword(s):  
Large Volume ◽  
Water Samples ◽  
Deep Sea ◽  
Sea Water ◽  
Survey Methods ◽  
Deep Ocean ◽  
Fish Diversity ◽  
Deep Sea Water ◽  
Sampling Protocols ◽  
Primer Sets

AbstractThe deep ocean is the largest biome on Earth and faces increasing anthropogenic pressures from climate change and commercial fisheries. Our ability to sustainably manage this expansive habitat is impeded by our poor understanding of its inhabitants and by the difficulties in surveying and monitoring these areas. Environmental DNA (eDNA) metabarcoding has great potential to improve our understanding of this region and to facilitate monitoring across a broad range of taxa. Here, we evaluate two eDNA sampling protocols and seven primer sets for elucidating fish diversity from deep sea water samples. We found that deep sea water samples (> 1400 m depth) had significantly lower DNA concentrations than surface or mid-depth samples necessitating a refined protocol with a larger sampling volume. We recovered significantly more DNA in large volume water samples (1.5 L) filtered at sea compared to small volume samples (250 mL) held for lab filtration. Furthermore, the number of unique sequences (exact sequence variants; ESVs) recovered per sample was higher in large volume samples. Since the number of ESVs recovered from large volume samples was less variable and consistently high, we recommend the larger volumes when sampling water from the deep ocean. We also identified three primer sets which detected the most fish taxa but recommend using multiple markers due the variability in detection probabilities and taxonomic resolution among fishes for each primer set. Overall, fish diversity results obtained from metabarcoding were comparable to conventional survey methods. While eDNA sampling and processing need be optimized for this unique environment, the results of this study demonstrate that eDNA metabarcoding can be employed to facilitate biodiversity surveys in the deep ocean, require less dedicated survey effort per unit identification and are capable of simultaneously providing valuable information on other taxonomic groups.

scholarly journals Metals of Deep Ocean Water Increase the Anti-Adipogenesis Effect of Monascus-Fermented Product via Modulating the Monascin and Ankaflavin Production

Marine Drugs ◽  
2016 ◽  
Vol 14 (6) ◽  
pp. 106 ◽  
Author(s):  
Tzu-Ying Lung ◽  
Li-Ya Liao ◽  
Jyh-Jye Wang ◽  
Bai-Luh Wei ◽  
Ping-Yi Huang ◽  
...  
Keyword(s):  
Deep Ocean ◽  
Ocean Water ◽  
Deep Ocean Water ◽  
Fermented Product

scholarly journals Characteristics of Tofu Coagulants Extracted from Sea Tangle Using Treated Deep Ocean Water

2007 ◽  
Vol 40 (3) ◽  
pp. 113-116 ◽  
Author(s):  
Seung-Won Lee ◽  
Hyeon-Joo Kim ◽  
Deok-Soo Moon ◽  
Ah-Ree Kim ◽  
In-Hak Jeong
Keyword(s):  
Deep Ocean ◽  
Ocean Water ◽  
Deep Ocean Water

scholarly journals Pararhodobacter marinus sp. nov., isolated from deep-sea water of the Indian Ocean

2019 ◽  
Vol 69 (4) ◽  
pp. 932-936 ◽  
Author(s):  
Qiliang Lai ◽  
Xiupian Liu ◽  
Jun Yuan ◽  
Shuchen Xie ◽  
Zongze Shao
Keyword(s):  
Indian Ocean ◽  
Deep Sea ◽  
Type Species ◽  
Sea Water ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Content Type ◽  
Deep Sea Water ◽  
Link Type ◽  
The Indian Ocean

A taxonomic study was carried out on strain CIC4N-9T, which was isolated from deep-sea water of the Indian Ocean. The bacterium was Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0–9% and at temperatures of 4–41 °C. The isolate was able to degrade gelatin but not aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CIC4N-9T belonged to the genus Pararhodobacter , with the highest sequence similarity to the only recognized species, Pararhodobacter aggregans D1-19T (96.9 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain CIC4N-9T and P. aggregans D1-19T were 80.4 and 23.0 %, respectively. The principal fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C16 : 0, C18 : 1ω7c 11-methyl, C18 : 0 and C17 : 0. The G+C content of the chromosomal DNA was 66.8 mol%. The sole respiratory quinone was determined to be Q-10. Phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unknown phospholipids, four unknown aminolipids and one unknown polar lipid were present. The combined genotypic and phenotypic data show that strain CIC4N-9T represents a novel species within the genus Pararhodobacter , for which the name Pararhodobacter marinus sp. nov. is proposed. The type strain is CIC4N-9T (=MCCC 1A01225T=KCTC 52336T).

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