Burkholderia gladioli infection in a pediatric patient with cystic fibrosis: the clinical challenges of an emergent pathogen

2021 ◽  
Vol 73 (5) ◽  
Author(s):  
Gilda BELLI ◽  
Mattia GIOVANNINI ◽  
Daniela DOLCE ◽  
Vito TERLIZZI ◽  
Tommaso ORIOLI ◽  
...  

1994 ◽  
Vol 34 (3) ◽  
pp. 353-361 ◽  
Author(s):  
I. N. Simpson ◽  
J. Finlay ◽  
D. J. Winstanley ◽  
N. Dewhurst ◽  
J. W. Nelson ◽  
...  


Author(s):  
Bradley S. Quon ◽  
James D. Reid ◽  
Patrick H. Wong ◽  
Pearce G. Wilcox ◽  
Amin R. Javer ◽  
...  


2000 ◽  
Vol 38 (1) ◽  
pp. 282-285
Author(s):  
Paul W. Whitby ◽  
Lauren C. Pope ◽  
Karen B. Carter ◽  
John J. LiPuma ◽  
Terrence L. Stull

ABSTRACT Burkholderia gladioli colonizes the respiratory tracts of patients with cystic fibrosis and chronic granulomatous disease. However, due to the high degree of phenotypic similarity between this species and closely related species in the Burkholderia cepacia complex, accurate identification is difficult. Incorrect identification of these species may have serious repercussions for the management of patients with cystic fibrosis. To develop an accurate procedure for the identification of B. gladioli , a molecular method to discriminate between this species and other species commonly isolated from the sputa of patients with cystic fibrosis was investigated. The 23S ribosomal DNA was cloned from several clinical isolates of B. gladioli , and the nucleotide sequence was determined. Computer-assisted sequence comparisons indicated four regions of the 23S rRNA specific for this species; these regions were used to design three primer pairs for species-specific PCR. Two of the primer pairs showed 100% sensitivity and specificity for B. gladioli when tested against a panel of 47 isolates comprising 19 B. gladioli isolates and 28 isolates of 16 other bacterial species. One of the primer pairs was further assessed for species specificity by using a panel of 102 isolates obtained from the Burkholderia cepacia Research Laboratory and Repository. The species-specific PCR was positive for 70 of 74 isolates of B. gladioli and was negative for all other bacterial species examined. Overall, this primer pair displayed a sensitivity and specificity of 96% (89 of 93) and 100%, respectively. These data demonstrate the potential of species-specific PCR for the identification of B. gladioli .



2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Zihao Yu ◽  
Guichun Yao ◽  
Maria Guadalupe Vizoso-Pinto ◽  
Lichang Sun ◽  
Ry Young ◽  
...  

ABSTRACT Burkholderia gladioli is a Gram-negative bacterium associated with cystic fibrosis infections. Here, we describe the genome sequence of B. gladioli phage Maja. Maja is most related to another Burkholderia phage, BcepF1, and may be a temperate phage, despite the absence of repressor or integrase homologs in its genome sequence.



Author(s):  
Annamaria Bagnasco ◽  
Giuseppe Aleo ◽  
Gianluca Catania ◽  
Milko Zanini ◽  
Loredana Sasso


2011 ◽  
Vol 18 (4) ◽  
pp. e64-e65 ◽  
Author(s):  
Bradley S Quon ◽  
James D Reid ◽  
Patrick Wong ◽  
Pearce G Wilcox ◽  
Amin Javer ◽  
...  

There have been very few reports describing postlung transplant outcomes in patients’ infected/colonized withBurkholderia gladiolipretransplant. A case involving a lung transplant recipient with cystic fibrosis who ultimately died as a result of severe rhinosinusitis due toB gladioliinfection in the context of postlung transplant immunosuppression is reported.





2019 ◽  
Vol 54 (4) ◽  
pp. E10-E12 ◽  
Author(s):  
Tuğba Şişmanlar Eyüboğlu ◽  
Ayşe T. Aslan ◽  
Tugba Ramaslı Gursoy ◽  
Zeynep R. Onay ◽  
Ulker Kocak ◽  
...  


CHEST Journal ◽  
2005 ◽  
Vol 128 (4) ◽  
pp. 152S ◽  
Author(s):  
Marcus P. Kennedy ◽  
Raymond D. Coakley ◽  
Scott H. Donaldson ◽  
Robert M. Aris ◽  
Kathy Hohneker ◽  
...  


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