scholarly journals AD946 and AF371 antibodies recognize a His-tagged recombinant protein by immunofluorescence

2020 ◽  
Vol 3 (2) ◽  
pp. e134
Author(s):  
Wanessa C Lima ◽  
Pierre Cosson
Keyword(s):  
Recombinant Protein ◽  
Hela Cells ◽  
His Tag

AD946 and AF371 antibodies against the His tag recognize a His-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

scholarly journals The AI196 antibody recognizes a SPOT-tagged recombinant protein by immunofluorescence

2020 ◽  
Vol 3 (2) ◽  
pp. e136
Author(s):  
Wanessa C Lima ◽  
Pierre Cosson
Keyword(s):  
Recombinant Protein ◽  
Hela Cells ◽  
Spot Tag

The AI196 antibody against the SPOT tag recognizes a SPOT-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

scholarly journals AE391 and AF291 antibodies recognize an HA-tagged recombinant protein by immunofluorescence

2020 ◽  
Vol 3 (2) ◽  
pp. e133
Author(s):  
Wanessa C Lima ◽  
Pierre Cosson
Keyword(s):  
Recombinant Protein ◽  
Hela Cells

AE391 and AF291 antibodies against the HA tag recognize an HA-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

scholarly journals The AI215 antibody recognizes an EPEA-tagged recombinant protein by immunofluorescence

2020 ◽  
Vol 3 (2) ◽  
pp. e130
Author(s):  
Wanessa C Lima ◽  
Pierre Cosson
Keyword(s):  
Recombinant Protein ◽  
Hela Cells

The AI215 antibody against the EPEA tag recognizes an EPEA-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

scholarly journals Cloning and expression of \(\textit{ pigI}\) gene in \(\textit{Escherichia coli}\) BL21 (DE3)

2021 ◽  
Vol 43 (3) ◽  
pp. 59-67
Author(s):  
Do Minh Trung ◽  
Do Hai Quynh ◽  
Nguyen Thuy Duong
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Biosynthetic Pathway ◽  
Condensation Reaction ◽  
Potential Method ◽  
Antimicrobial Activities ◽  
Cloning And Expression ◽  
E Coli ◽  
Recombinant Vector ◽  
His Tag

Prodigiosin (Pg), a secondary metabolite with anticancer and antimicrobial activities, can be produced in Serratia marcescens bacteria through the condensation reaction of 4-methoxy-2, 2’-bipyrrole-5-carboxyaldehyde (MBC) and 2-methyl-3-amylpyrrole (MAP). Among these, the MBC synthetic pathway is started by the conversion of L-proline to L-proline-AMP before this complex is covalently attached to PigG. This reaction is catalyzed by an L-prolyl-AMP ligase named PigI. Therefore, PigI protein plays an important role in the prodigiosin biosynthetic pathway. However, studies related to PigI protein have not been carried out in Vietnam yet. In this work, the pigI gene was cloned and expressed in Escherichia coli DH10B and BL21 (DE3), respectively. Sequence alignment results revealed that the obtained pigI gene is 99.7% identical to the four strains, CP027798, CP027796, CP021984 and CP003959. This recombinant vector pJET1.2/pigI was used to reamplify pigI, and the acquired amplicon was inserted into pET22b vector at the site of HindIII and XhoI. The clone E. coli BL21 (DE3) containing the recombinant vector pET22b/pigI was expressed in an auto-induced medium. The presence of PigI protein in the lysate was identified due to a 53 kDa band through Western Blot analysis using an anti-his-tag antibody. The results of our study provide a potential method for producing prodigiosin from recombinant protein in Vietnam.

A modified His-tag vector for the production of recombinant protein kinases

2008 ◽  
Vol 377 (2) ◽  
pp. 272-273 ◽  
Author(s):  
Rodrigo Lomas-Lopez ◽  
Alain J. Cozzone ◽  
Bertrand Duclos
Keyword(s):  
Recombinant Protein ◽  
Protein Kinases ◽  
His Tag

Expression of recombinant protein in cho and hela cells and its follow-up using EGF reporter gene

2006 ◽  
pp. 55-59
Author(s):  
V. Hendrick ◽  
D. Ribeiro Sousa ◽  
A. R. dos Santos Pedregal ◽  
C. Bassens ◽  
P. Rigaux ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Reporter Gene ◽  
Hela Cells

scholarly journals AF394, AF395 and AF396 antibodies recognize a GFP-tagged recombinant protein by immunofluorescence

2020 ◽  
Vol 3 (2) ◽  
pp. e131
Author(s):  
Wanessa C Lima ◽  
Pierre Cosson
Keyword(s):  
Recombinant Protein ◽  
Hela Cells

AF394, AF395 and AF396 antibodies against the GFP protein recognize a GFP-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

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