The AI196 antibody recognizes a SPOT-tagged recombinant protein by immunofluorescence

AF394, AF395 and AF396 antibodies against the GFP protein recognize a GFP-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

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AI239 and RB95 antibodies recognize a GST-tagged recombinant protein by immunofluorescence

Antibody Reports ◽

10.24450/journals/abrep.2020.e132 ◽

2020 ◽

Vol 3 (2) ◽

pp. e132

Author(s):

Wanessa C Lima ◽

Pierre Cosson

Keyword(s):

Recombinant Protein ◽

Hela Cells ◽

Glutathione S Transferase

AI239 and RB95 antibodies against the Glutathione S-transferase (GST) protein recognize a GST-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells; AF209, AF212 and RB94 do not.

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The AL626 antibody recognizes an ALFA-tagged recombinant protein by immunofluorescence

Antibody Reports ◽

10.24450/journals/abrep.2020.e129 ◽

2020 ◽

Vol 3 (2) ◽

pp. e129

Author(s):

Wanessa C Lima ◽

Pierre Cosson

Keyword(s):

Recombinant Protein ◽

Hela Cells

The AL626 antibody against the ALFA tag recognizes an ALFA-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells.

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AF166 and AI179 antibodies recognize a Myc-tagged recombinant protein by immunofluorescence

Antibody Reports ◽

10.24450/journals/abrep.2020.e135 ◽

2020 ◽

Vol 3 (2) ◽

pp. e135

Author(s):

Wanessa C Lima

Keyword(s):

Recombinant Protein ◽

Hela Cells

AF166 and AI179 antibodies against the Myc tag recognize a Myc-tagged human TAC protein by immunofluorescence in paraformaldehyde-fixed HeLa cells; AF372, AF373 and AI831 do not.

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Platinum Compounds as Stains for Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051165 ◽

1974 ◽

Vol 32 ◽

pp. 230-231

Author(s):

S. K. Aggarwal ◽

P. McAllister ◽

R. W. Wagner ◽

B. Rosenberg

Keyword(s):

Electron Microscopy ◽

Hela Cells ◽

Uranyl Acetate ◽

Sarcoma 180 ◽

Platinum Compounds ◽

Kb Cells ◽

En Bloc ◽

Human Lymphoma ◽

Ultrathin Sections ◽

Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

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Electron Microscopy of Mycoplasma Pneumoniae

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065341 ◽

1971 ◽

Vol 29 ◽

pp. 258-259 ◽

Cited By ~ 1

Author(s):

E. S. Boatman ◽

G. E. Kenny

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Growth Phase ◽

Hela Cells ◽

Sulfonic Acid ◽

Gamma Globulin ◽

Horse Serum ◽

Morphological Aspects ◽

The Family

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.

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