Analytic characterization of flow cytometric assays for detection of immunoglobulin G on canine erythroid cells, including detection of dog erythrocyte antigen 1 on erythroid precursors

2018 ◽  
Vol 79 (11) ◽  
pp. 1123-1132
Author(s):  
Cynthia A. Lucidi ◽  
John A. Gerlach ◽  
Susan Forney ◽  
L. Ari Jutkowitz ◽  
Michael A. Scott
Keyword(s):  
Immunoglobulin G ◽  
Erythroid Cells ◽  
Flow Cytometric ◽  
Erythroid Precursors ◽  
Erythrocyte Antigen ◽  
Dog Erythrocyte

Flow cytometric assessment of canine erythrocytes and platelets for dog erythrocyte antigen 1.1

2011 ◽  
Vol 40 (4) ◽  
pp. 435-443 ◽  
Author(s):  
Cynthia de A. Lucidi ◽  
Regina K. Takahira ◽  
John A. Gerlach ◽  
John M. Davis ◽  
Kenneth A. Schwartz ◽  
...  
Keyword(s):  
Flow Cytometric ◽  
Erythrocyte Antigen ◽  
Dog Erythrocyte

Flow cytometric quantitation and characterization of the T-lymphocyte memory response to CMV in healthy donors

Cytotherapy ◽  
2002 ◽  
Vol 4 (1) ◽  
pp. 29-40 ◽  
Author(s):  
N. Hensel ◽  
J.J. Melenhorst ◽  
K. Bradstock ◽  
A.P. Schwarer ◽  
R. Eniafe ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Memory Response ◽  
Flow Cytometric ◽  
Healthy Donors

scholarly journals Development, Manufacturing and Characterization of a Highly Purified, Liquid Immunoglobulin G Preparation from Human Plasma

2014 ◽  
Vol 41 (3) ◽  
pp. 205-212 ◽  
Author(s):  
Inga A. Laursen ◽  
Lene Blou ◽  
John S. Sullivan ◽  
Peter Bang ◽  
Flemming Balstrup ◽  
...  
Keyword(s):  
Human Plasma ◽  
Immunoglobulin G

scholarly journals Characterization of the gene and mRNA of the large subunit of ribulose-1,5-bisphosphate carboxylase in pea plants.

1983 ◽  
Vol 3 (4) ◽  
pp. 587-595 ◽  
Author(s):  
K K Oishi ◽  
K K Tewari
Keyword(s):  
Immunoglobulin G ◽  
Large Subunit ◽  
Rrna Genes ◽  
Mapping Technique ◽  
Affinity Column ◽  
Bisphosphate Carboxylase ◽  
Bamhi Fragment ◽  
Dna Fragment

mRNA coding for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase was obtained by fractionating chloroplast polysomes on an affinity column, using anti-ribulose-1,5-bisphosphate carboxylase immunoglobulin G. Approximately 20% of the polysomal RNA specifically bound to the affinity column. LS mRNA was also isolated by fractionating chloroplast polysomal RNA on sucrose gradients. The LS mRNA fraction was identified by translation in vitro followed by immunoprecipitation with anti-ribulose-1,5-bisphosphate carboxylase immunoglobulin G. Labeled LS mRNA was hybridized to a genomic digests of pea chloroplast DNA. The LS gene was localized on a 3.55-kilobase pair BamHI fragment in SalI-SmaI DNA fragment 4. The BamHI fragment containing the LS gene was cloned, and a restriction endonuclease map was constructed. The LS gene was localized on a 1.9-kbp KpnI-EcoRI fragment. The LS gene was analyzed by electron microscopy, using the R loop mapping technique. LS mRNA was colinear with the gene, and its size was 1.35 +/- 0.2 kilobase pairs. When the LS mRNA was analyzed on methylmercury agarose gels, it comigrated with the 16S rRNA. The direction of transcription of the LS gene was in the same direction as that of the rRNA genes.

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