Is Testudo werneri a distinct species?

Biologia ◽  
2007 ◽  
Vol 62 (2) ◽  
Author(s):  
Pavel Široký ◽  
Uwe Fritz
Keyword(s):  
Cytochrome B ◽  
Sequence Variation ◽  
Distinct Species ◽  
Cytochrome B Gene ◽  
Nominal Species ◽  
Common Haplotype ◽  
Pet Trade ◽  
Adjacent Part ◽  
Pooled Sample

AbstractSequence variation of a 1066 bp long mtDNA fragment (cytochrome b gene, adjacent part of tRNA-Thr gene) of four known-locality samples of Testudo kleinmanni (Tripolitania, Libya) and of four samples of T. werneri (Negev, Israel) is compared with additional five sequences of pet trade tortoises allegedly representing T. kleinmanni. Four haplotypes, differing in one to four mutation steps occur. The most common haplotype was shared by all known-locality samples of T. kleinmanni and three T. werneri. Sequence variation within each nominal species and in the pooled sample of T. kleinmanni, T. werneri and pet trade tortoises is the lowest known for any Testudo species. We conclude there is no support for the validity of T. werneri Perälä, 2001.

scholarly journals Molecular phylogeny of East Asian moles inferred from the sequence variation of the mitochondrial cytochrome b gene.

2000 ◽  
Vol 75 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Kimiyuki Tsuchiya ◽  
Hitoshi Suzuki ◽  
Akio Shinohara ◽  
Masashi Harada ◽  
Shigeharu Wakana ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Cytochrome B ◽  
Sequence Variation ◽  
East Asian ◽  
Cytochrome B Gene ◽  
Mitochondrial Cytochrome ◽  
Mitochondrial Cytochrome B ◽  
Mitochondrial Cytochrome B Gene

Molecular Relationships Within Australasian Waterfowl (Anseriformes)

1996 ◽  
Vol 44 (1) ◽  
pp. 47 ◽  
Author(s):  
M Sraml ◽  
L Christidis ◽  
S Easteal ◽  
P Horn ◽  
C Collet
Keyword(s):  
Cytochrome B ◽  
Endemic Species ◽  
Base Pair ◽  
Phylogenetic Relationships ◽  
Sequence Variation ◽  
Cytochrome B Gene ◽  
Base Pair Fragment ◽  
Molecular Relationships ◽  
Pair Fragment ◽  
Controversial Nature

Within Australia there are 19 endemic species of Anseriformes. Six of these belong to monotypic genera, some of which remain controversial with respect to phylogenetic relationships. Sequence variation in a 307-base pair fragment of the cytochrome-b gene was compared from 23 species of waterfowl (the Cairina sequence was obtained from the literature) to elucidate further the relationships of these monotypic Australian genera. Anseranas and Dendrocygna were identified as the earliest diverged genera among the taxa examined. The remaining genera fell into two groups: (1) Tadorna, Alopochen, Chenonetta, Anas, Aythya, Cairina and Air and (2) Cygnus, Branta, Cereopsis, Biziura, Oxyura, Malacorhynchus, Stictonetta and Nettapus. The controversial nature of the last group is discussed.

Tawny Owl (Strix aluco) and Hume's Tawny Owl (Strix butleri) Are Distinct Species: Evidence from Nucleotide Sequences of the Cytochrome b Gene

1994 ◽  
Vol 49 (3-4) ◽  
pp. 230-234 ◽  
Author(s):  
Petra Heidrich ◽  
Michael Wink
Keyword(s):  
Cytochrome B ◽  
Distinct Species ◽  
Cytochrome B Gene ◽  
Tyto Alba ◽  
Nucleotide Substitutions ◽  
Base Pairs ◽  
Tawny Owl ◽  
Strix Aluco ◽  
Parsimony Principle ◽  
Athene Noctua

The cytochrome b gene of the Tawny Owl (Strix aluco), Hume’s Tawny Owl (Strix butleri) and the African wood owl (Strix woodfordii) was amplified by polymerase chain reaction (PCR) and partially sequenced (300 base pairs). Sequences differ substantially (9 to 12% nucleotide substitutions) between these taxa indicating that they represent distinct species, which is also implicated from morphological and biogeographic differences. Using cytochrome b sequences of S. aluco, S. butleri, S. woodfordii, Athene noctua and Tyto alba phylogenetic relationship were reconstructed using the “maximum parsimony” principle (PAUP 3.1.1) and the neighbour-joining method (MEGA)

Genetic structure of the Siberian Sucker (Catostomus catostomus rostratus) according to data on sequence variation of the mtDNA cytochrome b gene

2014 ◽  
Vol 41 (4) ◽  
pp. 306-311 ◽  
Author(s):  
L. T. Bachevskaya ◽  
V. V. Pereverzeva ◽  
G. D. Ivanova ◽  
G. A. Agapova ◽  
A. A. Primak
Keyword(s):  
Genetic Structure ◽  
Cytochrome B ◽  
Sequence Variation ◽  
Cytochrome B Gene ◽  
Catostomus Catostomus

Molecular Phylogeny of South American Screech Owls of the Otus atricapillus Complex (Aves: Strigidae) Inferred from Nucleotide Sequences of the Mitochondrial Cytochrome b Gene

1995 ◽  
Vol 50 (3-4) ◽  
pp. 294-302 ◽  
Author(s):  
Petra Heidrich ◽  
Claus König ◽  
Michael Wink
Keyword(s):  
Cytochrome B ◽  
Distance Matrix ◽  
Single Species ◽  
Distinct Species ◽  
Nucleotide Sequences ◽  
Cytochrome B Gene ◽  
Character State ◽  
South American ◽  
Nucleotide Substitutions ◽  
Mitochondrial Cytochrome B

The cytochrome b gene of 6 South American screech owls of the genus Otus (O. choliba, O. atricapillus, O. usta, O. sanctaecatarinae, O. guatemalae, and O. hoyi) and two Old World species (Otus scops and Otus leucotis) was amplified by polymerase chain reaction (PCR) and partially sequenced (300 nucleotides). Otus atricapillus, O. guatemalae, O. hoyi and O. sanctaecatarinae which are morphologically very similar, have been treated as belonging to a single species, A. atricapillus (Sibley and Monroe, 1990). Nucleotide sequences differ substantially between these taxa (6.3 to 8.8% nucleotide substitutions) indicating that they represent well established and distinct species which had been implicated already from ecological and bioacoustical analyses (König, 1991, 1994). The importance of vocal and ecological characters for the taxonomy of nocturnal birds is thus confirmed by our molecular analysis. Phylogenetic relationships were reconstructed between Old and New World owls using character state (“maximum parsimony”; PAUP 3.1.1) and distance matrix methods (neighbour- joining; MEGA).

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