scholarly journals Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae)

2019 ◽  
Vol 68 (1) ◽  
pp. 26-28 ◽  
Author(s):  
Zhaohong Ding ◽  
Jianbo Lu
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Magnetic Beads ◽  
Genomic Library ◽  
Landscape Fragmentation ◽  
Isolation And Characterization ◽  
Bioenergy Plant ◽  
Polymorphic Microsatellite

Abstract Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.

scholarly journals Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1286-1287 ◽  
Author(s):  
Long-na Li ◽  
Songjun Zeng ◽  
Feng Zheng ◽  
Zhi-lin Chen ◽  
Kun-lin Wu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Microsatellite Locus ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Relationship Patterns ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

scholarly journals Isolation and Characterization of Nine Microsatellite Markers for Cymbidium sinense

HortScience ◽  
2008 ◽  
Vol 43 (6) ◽  
pp. 1925-1926 ◽  
Author(s):  
Kuaifei Xia ◽  
Xiulin Ye ◽  
Mingyong Zhang
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Loci ◽  
Molecular Breeding ◽  
Magnetic Beads ◽  
Genomic Library ◽  
Germplasm Resources ◽  
Enrichment Method ◽  
Isolation And Characterization ◽  
Cymbidium Sinense

Microsatellite-enhanced genomic library of Cymbidium sinense was constructed using repeat-enrichment method with biotin-labeled oligos and streptavidin magnetic beads. Twenty-five microsatellite loci were isolated from a microsatellite-enhanced genomic library of Cymbidium sinense. Nine of these loci displayed genetic diversity that ranged from one to 15 alleles per locus, and the observed heterozygosity of six polymorphic loci ranged from 0.18 to 0.90 with an average of 0.47 (0.29 sd) in a sample of 30 individuals. Eight of these loci were successfully amplified in at least one of the following species: Paphiopedilum hissutissimum, P. wardii, P. armeniacum, or P. micranthum. The study provides a base for molecular breeding and assessment of germplasm resources of the Cymbidium sinense and the results suggest that the microsatellite loci developed from C. sinense may have a broad applicability within the Orchidaceae family.

Applications of microsatellite markers in studies of genetics and breeding of fish

2006 ◽  
Vol 3 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Zhang Xiao-Gu ◽  
Tong Jin-Gou ◽  
Xiong Bang-Xi
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Population Genetic ◽  
Germplasm Conservation ◽  
Genomic Mapping ◽  
Short Sequence ◽  
Sequence Repeat ◽  
Short Sequence Repeat ◽  
Food Fish ◽  
Polymorphic Microsatellite

AbstractThe microsatellite, or short sequence repeat (SSR), is a powerful genetic marker, useful in many areas of fish genetics and breeding. Polymorphic microsatellite loci have been frequently applied to the analysis of genetic diversity, population genetic structure, and genomic mapping. These co-dominant markers have also been applied to the classification and systematics, parentage identification, germplasm conservation, and breeding programme of food fish.

Population genetic structure of wild and farmed rusa deer (Cervus timorensis russa) in New-Caledonia inferred from polymorphic microsatellite loci

Genetica ◽  
2009 ◽  
Vol 137 (3) ◽  
pp. 313-323 ◽  
Author(s):  
M. de Garine-Wichatitsky ◽  
T. de Meeûs ◽  
C. Chevillon ◽  
D. Berthier ◽  
N. Barré ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Population Genetic Structure ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
New Caledonia ◽  
Polymorphic Microsatellite

Highly polymorphic microsatellite markers in Chamaecyparis obtusa

2001 ◽  
Vol 31 (12) ◽  
pp. 2248-2251 ◽  
Author(s):  
Y Nakao ◽  
H Iwata ◽  
A Matsumoto ◽  
Y Tsumura ◽  
N Tomaru
Keyword(s):  
Microsatellite Loci ◽  
Magnetic Beads ◽  
Genomic Library ◽  
Gene Diversity ◽  
Pcr Amplification ◽  
Chamaecyparis Obtusa ◽  
Mendelian Inheritance ◽  
Average Gene Diversity ◽  
Polymorphic Microsatellite ◽  
Average Gene

Nine microsatellite loci in hinoki, Chamaecyparis obtusa (Sieb. et Zucc.) Endl., were identified and characterized. A genomic library, developed using enrichment with magnetic beads, was screened to identify microsatellite repeats (CT/AG). The microsatellite loci, where the alleles were segregated, displayed codominant Mendelian inheritance. Genetic analysis of 16 plus trees and two unrelated individuals of Chamaecyparis obtusa revealed that all loci were highly polymorphic, with an average of 10.3 alleles per locus, and an average gene diversity of 0.77. The applicability of these microsatellite loci was also tested in other species of the Cupressaceae and in Cryptomeria japonica (L.f.) D. Don (Taxodiaceae, a family closely related to Cupressaceae). Polymerase chain reaction (PCR) amplification was successful for about half of the loci of the species in the genus Chamaecyparis. However, the PCR amplification patterns of the 11 species of Cupressaceae showed no clear correlations with their molecular phylogeny. The highly polymorphic microsatellite loci in Chamaecyparis obtusa, identified here, will be useful in studies of hinoki breeding and population genetics.

scholarly journals Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 690-692 ◽  
Author(s):  
Jiang-Chong Wu ◽  
Jing Yang ◽  
Zhi-Jian Gu ◽  
Yan-Ping Zhang
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Moringa Oleifera ◽  
Microsatellite Primers ◽  
Genetic Studies ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

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