Assessment of Slow Rusting Resistance Components to Stripe Rust Pathogen in some Exotic Wheat Germplasm

Barberry (Berberis spp.) is an alternate host for both the stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), and the stem rust pathogen, P. graminis f. sp. tritici (Pgt), infecting wheat. Infection risk was assessed to determine whether barberry could be infected by either of the pathogens in Asia and Southeastern Europe, known for recurring epidemics on wheat and the presence of barberry habitats. For assessing infection risk, mechanistic infection models were used to calculate infection indices for both pathogens on barberry following a modeling framework. In East Asia, Bhutan, China, and Nepal were found to have low risks of barberry infection by Pst but high risks by Pgt. In Central Asia, Azerbaijan, Iran, Kazakhstan, southern Russia, and Uzbekistan were identified to have low to high risks of barberry infection for both Pst and Pgt. In Northwest Asia, risk levels of both pathogens in Turkey and the Republic of Georgia were determined to be high to very high. In Southwest Asia, no or low risk was found. In Southeastern Europe, similar high or very high risks for both pathogens were noted for all countries. The potential risks of barberry infection by Pst and/or Pgt should provide guidelines for monitoring barberry infections and could be valuable for developing rust management programs in these regions. The framework used in this study may be useful to predict rust infection risk in other regions.

Download Full-text

Overwintering potential of the stripe rust pathogen (Puccinia striiformis) in central Alberta

Canadian Journal of Plant Pathology ◽

10.1080/07060661.2013.809385 ◽

2013 ◽

Vol 35 (3) ◽

pp. 304-314 ◽

Cited By ~ 11

Author(s):

K. Kumar ◽

M. D. Holtz ◽

K. Xi ◽

T. K. Turkington

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Rust Pathogen

Download Full-text

Inheritance and Molecular Mapping of Barley Genes Conferring Resistance to Wheat Stripe Rust

Phytopathology ◽

10.1094/phyto-95-0884 ◽

2005 ◽

Vol 95 (8) ◽

pp. 884-889 ◽

Cited By ~ 22

Author(s):

Vihanga Pahalawatta ◽

Xianming Chen

Keyword(s):

Resistance Gene ◽

Microsatellite Marker ◽

Stripe Rust ◽

Molecular Mapping ◽

Puccinia Striiformis ◽

Infection Type ◽

Dominant Gene ◽

Wheat Stripe Rust ◽

Rust Pathogen ◽

Type Data

Most barley cultivars are resistant to stripe rust of wheat that is caused by Puccinia striiformis f. sp. tritici. The barley cv. Steptoe is susceptible to all identified races of P. striiformis f. sp. hordei (PSH), the barley stripe rust pathogen, but is resistant to most P. striiformis f. sp. tritici races. To determine inheritance of the Steptoe resistance to P. striiformis f. sp. tritici, a cross was made between Steptoe and Russell, a barley cultivar susceptible to some P. striiformis f. sp. tritici races and all tested P. striiformis f. sp. hordei races. Seedlings of parents and F1, BC1, F2, and F3 progeny from the barley cross were tested with P. striiformis f. sp. tritici races PST-41 and PST-45 under controlled greenhouse conditions. Genetic analyses of infection type data showed that Steptoe had one dominant gene and one recessive gene (provisionally designated as RpstS1 and rpstS2, respectively) for resistance to races PST-41 and PST-45. Genomic DNA was extracted from the parents and 150 F2 plants that were tested for rust reaction and grown for seed of F3 lines. The infection type data and polymorphic markers identified using the resistance gene analog polymorphism (RGAP) technique were analyzed with the Mapmaker computer program to map the resistance genes. The dominant resistance gene in Steptoe for resistance to P. striiformis f. sp. tritici races was mapped on barley chromosome 4H using a linked microsatellite marker, HVM68. A linkage group for the dominant gene was constructed with 12 RGAP markers and the microsatellite marker. The results show that resistance in barley to the wheat stripe rust pathogen is qualitatively inherited. These genes might provide useful resistance against wheat stripe rust when introgressed into wheat from barley.

Download Full-text

Incursions of divergent genotypes, evolution of virulence and host jumps shape a continental clonal population of the stripe rust pathogen Puccinia striiformis

Molecular Ecology ◽

10.1111/mec.16182 ◽

2021 ◽

Author(s):

Yi Ding ◽

Will S. Cuddy ◽

Colin R. Wellings ◽

Peng Zhang ◽

Tine Thach ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Clonal Population ◽

Evolution Of Virulence ◽

Rust Pathogen

Download Full-text

Isolation and characterization of yellow rust resistant mutants in wheat.

10.1079/9781789249095.0010 ◽

2021 ◽

pp. 103-110

Author(s):

Suman Bakshi ◽

Johar Singh ◽

Sanjay J. Jambhulkar

Keyword(s):

Electron Beam ◽

Stripe Rust ◽

Gamma Ray ◽

Yellow Rust ◽

Mutation Breeding ◽

Adult Plant ◽

Adult Plant Stage ◽

Rust Pathogen ◽

Plant Stage ◽

Resistant Mutants

Abstract Stripe rust, also known as yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat production leading to yield losses up to 84%. Due to climate change, new races of the yellow rust pathogen are appearing for which no durable source of resistance has been observed in the present high-yielding varieties. A mutation breeding programme was initiated in two popular varieties, namely PBW343 and HD2967, using gamma-ray and electron beam irradiation. Gamma-ray doses of 250, 300 and 350 Gy and electron beam doses of 150, 200 and 250 Gy were used for seed irradiation. The M2 population was screened in the field from seedling to adult plant stage by spraying a mixture of urediniospores of Pst pathotypes. Disease severity was recorded as the percentage of leaf area covered by the rust pathogen following a modified Cobb's scale. A total of 52 putative yellow rust resistant mutants in HD2967 and 63 in PBW343 were isolated. The number of mutants was higher in the electron beam irradiated population compared with gamma-rays. The absence of sporulation and spore production of the rust pathogen on the mutants indicated resistance. Mutant plants showing seedling resistance also showed resistance at adult plant stage. Seed yield and its contributing characters were better in the mutants compared with the parents. These rust resistant mutants could be novel sources of stripe rust or yellow rust resistance. The plant-to-row progenies of these mutants were confirmed and characterized in the M3 generation.

Download Full-text

Pathogens which threaten food security: Puccinia striiformis, the wheat stripe rust pathogen

Food Security ◽

10.1007/s12571-020-01016-z ◽

2020 ◽

Vol 12 (2) ◽

pp. 239-251 ◽

Cited By ~ 9

Author(s):

Xianming Chen

Keyword(s):

Food Security ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Wheat Stripe Rust ◽

Rust Pathogen

Download Full-text

Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici

Molecular Biology Reports ◽

10.1007/s11033-009-9823-9 ◽

2009 ◽

Vol 37 (2) ◽

pp. 1045-1052 ◽

Cited By ~ 45

Author(s):

Bo Liu ◽

Xiaodan Xue ◽

Suping Cui ◽

Xiaoyu Zhang ◽

Qingmei Han ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Rust Pathogen

Download Full-text

Quantitative Trait Loci for High-Temperature Adult-Plant and Slow-Rusting Resistance to Puccinia striiformis f. sp. tritici in Wheat Cultivars

Phytopathology ◽

10.1094/phyto-98-7-0803 ◽

2008 ◽

Vol 98 (7) ◽

pp. 803-809 ◽

Cited By ~ 43

Author(s):

Q. Guo ◽

Z. J. Zhang ◽

Y. B. Xu ◽

G. H. Li ◽

J. Feng ◽

...

Keyword(s):

High Temperature ◽

Stripe Rust ◽

Phenotypic Variation ◽

Quantitative Trait ◽

Puccinia Striiformis ◽

Infection Type ◽

Adult Plant ◽

Wheat Cultivars ◽

Slow Rusting ◽

Adult Plants

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most damaging diseases of wheat (Triticum aestivum) globally. High-temperature adult-plant resistance (HTAPR) and slow-rusting have great potential for sustainable management of the disease. The wheat cultivars Luke and Aquileja have been previously reported to possess HTAPR and slow-rusting to stripe rust, respectively. Aquileja displayed less number of stripes per unit leaf area than Luke, while Luke showed lower infection type than Aquileja at adult-plant stages of growth under high-temperature conditions. The objectives of this study were to confirm the resistances and to map the resistance genes in Luke and Aquileja. Luke was crossed with Aquileja, and 326 of the F2 plants were genotyped using 282 microsatellite primer pairs. These F2 plants and their derived F3 families were evaluated for resistance to stripe rust by inoculation in the fields and greenhouses of high- and low-temperatures. Infection type was recorded for both seedlings and adult plants, and stripe number was recorded for adult plants only. Two quantitative trait loci (QTL) were identified, on the short arm of chromosome 2B, to be significantly associated with infection type at adult-plant stages in the fields and in the high-temperature greenhouse. The locus distal to centromere, referred to as QYrlu.cau-2BS1, and the locus proximal to centromere, referred to as QYrlu.cau-2BS2, were separated by a genetic distance of about 23 cM. QYrlu.cau-2BS1 was flanked by the microsatellite markers Xwmc154 and Xgwm148, and QYrlu.cau-2BS2 was flanked by Xgwm148 and Xabrc167. QYrlu.cau-2BS1 and QYrlu.cau-2BS2 explained up to 36.6 and 41.5% of the phenotypic variation of infection type, respectively, and up to 78.1% collectively. No significant interaction between the two loci was detected. Another QTL, referred to as QYraq.cau-2BL, was detected on the long arm of chromosome 2B to be significantly associated with stripe number. QYraq.cau-2BL was flanked by the microsatellite markers Xwmc175 and Xwmc332, and it explained up to 61.5% of the phenotypic variation of stripe number. It is possible that these three QTL are previously unmapped loci for resistance to stripe rust.

Download Full-text