scholarly journals ALTERNATIF PENGGUNAAN SILIKA BENTONIT SEBAGAI PENGGANTI FENOL-KLOROFORM-ISOAMIL ALKOHOL DALAM EKSTRAKSI DNA SECARA LANGSUNG DARI TANAH HUTAN MANGROVE

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Khisan Qamariya ◽  
Wahyu Dwijani Sulihingtyas ◽  
I Nengah Wirajana
Keyword(s):  
Humic Acid ◽  
Gel Electrophoresis ◽  
Mangrove Forest ◽  
Isoamyl Alcohol ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Metagenomic Dna ◽  
Alcohol Extract ◽  
Purity Level ◽  
Relative Purity

Research on comparing the metagenomic DNA extraction from mangrove forest soil with phenol-chloroform-isoamyl alcohol and with silica bentonite was conducted. The purpose of this study was to find the differences in quality and quantity of metagenomic DNA extracted. The silica bentonite was compared to meet the Green Chemistry concept. The metagenomic DNA from these extractions were analyzed by agarose gel electrophoresis and UV-Vis spectrophotometry. The results of electrophoresis showed that metagenomic DNA extracted with silica bentonite were relatively higher in concentration than with phenol-chloroform-isoamyl alcohol; but integrity of their DNA were the same. The results of UV-Vis spectrophotometry analysis showed that A260/230 ratio of the phenol-chloroform-isoamyl alcohol extract had higher relative purity level to humic acid. The A260/280 ratio of the final elution of the silica bentonite extraction showed the highest relative purity level to protein.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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